DreINT0099874 @ danRer10
Intron Retention
Gene
ENSDARG00000076075 | myh7ba
Description
myosin, heavy chain 7B, cardiac muscle, beta a [Source:ZFIN;Acc:ZDB-GENE-100914-2]
Coordinates
chr11:24969364-24969692:-
Coord C1 exon
chr11:24969589-24969692
Coord A exon
chr11:24969503-24969588
Coord C2 exon
chr11:24969364-24969502
Length
86 bp
Sequences
Splice sites
5' ss Seq
GATGTAAGC
5' ss Score
6.16
3' ss Seq
CCCTTCTCTTTCTTTTCCAGCAT
3' ss Score
12.04
Exon sequences
Seq C1 exon
ACATGCTACTGGTCTCTTCAAACCCATATGACTACCACTTTTGCTCCCAAGGAGTGACAACGGTGGAAAATATGGATGATGGACAGGAACTTATGGCTACTGAT
Seq A exon
GTAAGCTGTAAATTCTTATTATCTCATCGCCTACAAAGACCAACTAAAAAAAAACAGGCAGCAAAACCCTTCTCTTTCTTTTCCAG
Seq C2 exon
CATGCCATGGACATCCTGGGCTTCACTCCAGAAGAAAAGTACGGCTGCTATAAAATAGTAGGGGCCATCATGCACTTCGGGAACATGAAATTCAAACAGAAGCAGAGGGAGGAGCAGGCAGAGGCTGATGGCACTGAAA
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000076075:ENSDART00000097316:11
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.057 A=NA C2=0.213
Domain overlap (PFAM):
C1:
PF0006316=Myosin_head=FE(4.9=100)
A:
NA
C2:
PF0006316=Myosin_head=FE(6.7=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CAAGGAGTGACAACGGTGGAA
R:
GCTCCTCCCTCTGCTTCTGTT
Band lengths:
172-258
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]