BtaEX0032455 @ bosTau6
Exon Skipping
Gene
ENSBTAG00000046548 | SIAT7B
Description
Bos taurus ST6 (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1, 3)-N-acetylgalactosaminide alpha-2,6-sialyltransferase 4 (ST6GALNAC4), mRNA. [Source:RefSeq mRNA;Acc:NM_205791]
Coordinates
chr11:98592423-98594798:-
Coord C1 exon
chr11:98594678-98594798
Coord A exon
chr11:98594165-98594250
Coord C2 exon
chr11:98592423-98592608
Length
86 bp
Sequences
Splice sites
3' ss Seq
GAGTGTCTGGGTCTTGGCAGGTC
3' ss Score
6.72
5' ss Seq
CCGGTAAGT
5' ss Score
10.91
Exon sequences
Seq C1 exon
CGGCGACGGCGACGCCTCAGCGCCGGGGCTCCGGCTTCCTGCCCCGTCCGTCCCGCCCGCGGGCCATGGAGCTCCGAGTAACGGATGGGGAGCAGCCGGCGCATCCCCGCCTGCCCTCCAG
Seq A exon
GTCCCTGGCTCCCATCCCTTCCCAGCCTGATCCCTGGAGGCAGCAGCCACGGGCTACTGAGCGGCAGTGACAGCATGAAGGCTCCG
Seq C2 exon
TGTCGGCTCCTGCTCATCGTCCTCTGCTCTGTGGGCTTCTCGGCTGTCTATATCCTGCTGTGCTACTGGGCCTGCCTGCCCTTCTGCCCAGCCTTCTGCCTGGACCCCCACCTCTCTATCAAGTCCAGGCCCACCGTGCCAGGGCCTCTGCACTTCAGTGGCTACAGCAGCGTGCCAGATGGGAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000046548_MULTIEX1-1/6=C1-2
Average complexity
S*
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
PF0077713=Glyco_transf_29=PU(10.6=19.4)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GCCATGGAGCTCCGAGTAAC
R:
GATAGAGAGGTGGGGGTCCAG
Band lengths:
179-265
Functional annotations
There are 1 annotated functions for this event
PMID: 32249828
[CRISPR screen]. This alternative exon is implicated in cell fitness by a CRISPR-based exon deletion phenotypic screen in RPE-1 cells.
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development