BtaINT0013081 @ bosTau6
Intron Retention
Gene
ENSBTAG00000017258 | ACSL3
Description
acyl-CoA synthetase long-chain family member 3 [Source:HGNC Symbol;Acc:HGNC:3570]
Coordinates
chr2:111866440-111867886:+
Coord C1 exon
chr2:111866440-111866617
Coord A exon
chr2:111866618-111867776
Coord C2 exon
chr2:111867777-111867886
Length
1159 bp
Sequences
Splice sites
5' ss Seq
AGCGTACGT
5' ss Score
7.8
3' ss Seq
GCCCTTTCTTTTCCCTGTAGTTG
3' ss Score
9.93
Exon sequences
Seq C1 exon
GTTATTCTTGGAAGCTATAATTGGCTTTCCTACGAAGATGTCTTTGTGAGAGCCTTTAATTTTGGAAACGGCTTACAGATGCTGGGTCAGAAGCCCAAGACCAACATCGCCATCTTCTGTGAGACCAGGGCAGAGTGGATGGTTGCTGCACAGGCCTGCTTCATGTATAATTTTCAGC
Seq A exon
GTACGTGGGCTTCCTTACCTTCTTGAAGCATGGACCTGGTGTTGTGGGAGAGATGATACTTACTATTTATGATAAGTATTTCCTCCCAGGATAAAATTCTGATGTTAATTTTAACCTTTGTTACAATTTAAGACATACTGGTTTGTTCAGTTGTGGAACAAATTACCCGTGTAGTAAGAGGACTTGAACCTATTCTGAGGGGACTTTGTGGGTCTGGGGAACTAAATCTACACCTGTGGGTCATTGCTCTGCTACCCCTTCTCAGAATTGTCCTTTAAAGTTAGTGTCATCTTGTTAGGCCATTTTTACAGTTGACAGCTGGCCTCTAAAGCACTTCCAACACTCAAACCTAGCTGCTAGTTAAACGATAGAGACTTTTTATGTTGTATAAATATAAGACGACTTGTGGAAGTTGTGATGCCTGTGAGGTCGTCTGAGAAGTTTCGTTCAGAGGTTATGGTGTCAGTTGTGGTATTAAACATGGACATTGTTTTATACTTCAGATGAAATGGAAAGGATACTTTTTATCATCAAATTTCTTTTTGTAAATGCCTCCACTGTATTTTGTTTTTAAACTGGTATACCTTTTTGTTTTCATAGGTTTGGGTTTATAGTATGTTTCTTTTAAGAAGCTTTAAATTATCTCTTAAAGTGACTTTGTTTAACTTCTCATTATTATTTAATTTTGACATTTCTAAGACTATTTTTCTTAAAATTTTTCTACTTTAGAATAAGTAGTATTACCACTCATTAGTGCTATTTTTTAAAGAAATAATATCTTAAATATGACATTCAGTGTCTTATTTTATTGGTATATTTTTTATATTTTGGTATCAAATAGTTAACATCTATGACTTTGACATTTTAGAAGTCAAATATAATTCCTTTCAACATCACAGTTTCAGAGTAGCATCTGTAGGTGAAAGAAAACAAAAAAATTTTTAAATGTTGAAATTTTGTGGGGGAATTATAGAAGAGTGAGTAGGCGTCATGTTAATATATTAAAATAATTTAGAATAACAGCACAATTAGACCACAAATTATGGATCTAACATTTTAGGGTTTAGATGTTCCGTCAGTGGTCAGAGGCTTTATTTAATTGCACTTGGTGCTTCTATGAAATGTCAAGTATTTGACTTGCCCTTTCTTTTCCCTGTAG
Seq C2 exon
TTGTTACGCTGTATGCTACTCTAGGGGGTCCAGCAATTGTTCATGGATTAAATGAAACAGAGGTGACCAACATCATTACTAGTAAAGAACTCTTGCAGACAAAATTGAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000017258:ENSBTAT00000022939:3
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0050123=AMP-binding=FE(12.4=100)
A:
NA
C2:
PF0050123=AMP-binding=FE(7.6=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GTTATTCTTGGAAGCTATAATTGGCT
R:
TCAATTTTGTCTGCAAGAGTTCTT
Band lengths:
286-1445
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]