BtaINT0032782 @ bosTau6
Intron Retention
Gene
ENSBTAG00000004267 | CCDC148
Description
coiled-coil domain containing 148 [Source:HGNC Symbol;Acc:HGNC:25191]
Coordinates
chr2:38092330-38093895:+
Coord C1 exon
chr2:38092330-38092392
Coord A exon
chr2:38092393-38093773
Coord C2 exon
chr2:38093774-38093895
Length
1381 bp
Sequences
Splice sites
5' ss Seq
CAGGTATTT
5' ss Score
7.51
3' ss Seq
CTGTTTTTGATGAAAAACAGATA
3' ss Score
4.15
Exon sequences
Seq C1 exon
GTATGGAAAACATGAATAAAAGAGACCAACTATTCATGACAATTCAGAAAAATGATAGCTCAG
Seq A exon
GTATTTTTCCTACTTCTAAGCTTATTTTATGGTTTATGGTAGATTATGGGTGATATAAAACTAGAACGTTGATTTGCAAAATTTTAATAATGGAAGGTTCATTTTTTTGGTCATAGTGAAAATTTCTTTGGTTTCCTCACAATAATCAGACTAATCATTCTTTCCTTCACTGTGCTTGTCCTTAAGTCATTTGGAGCCTTCTTCCCATCATGTGACCCATGATATACTTAATAAATTTTAGTTGCCTTACATTGCACAGGTGAATTTTTCACATCATTGAGGTAATGATGCTTGAATTACTCAAATTTTTGTACTTCATGTTTCTTCGCCTTGGAGTTCAGTTCATTCAGTTCAGTTGCTCAGTCGTGTCCAACTCTTTGTGACCCCATGAACTGCAGCATGCCAGGCCTCCCTGTCCATCACCAATTCCCAGAGTTTACCCAAACTCATGTCCGTTGAGTCGGTGATGCCATCCAACCAGCTCATCTTCTGTCGTCCCTTTCTCCTGCCCTCAATCTTTCCCAGCATCAGGGTCTTTTCCAATGAGTCAGCTCTTTGCATCAGCTGGCCAAAGTATTGGAGTTTCAGCTTCAACATCAGTCCTTCCAATGAACACCCAGGACTGATCTCCTTTAGGATGGACTGGTTGGATCTCCTTGCAGTCCAAGGGACTCTCAACAGTCTTCTGCAACATCACAGTTCAAAAGCATCGATTCTTCGGCCCTCAGCTTTATAGTCCAACTCTCACATCCATACATGACCACTGGAAAAACCATAGCCTTGACTATCCTCTACCTGTATTTTATTTTATCAAAATAGTTAATATCAAGCTAAACTAGGACATTTTATATAGTTGGATGGAGAATGCCTGCCCACACATTTTATATCATTTTTTGTCTTATCTGGCCTATTAAATTTCTTAGCTATCAGTTTCAACCTCTTTTGTATTTAACTGAGTCAATTTTTCTAGGTTAAAAATGCAACTATGACTTTAAAAGTTCTATTTACTTAATTTGATTCATATGAATGTGATACTTACATATTCATAGTTAATAACTAACACATTTTAAATAAAAAGAATGATCACACGTTGTATCTTGACAGTATAATTTTCTAAAATTCTCAATATATTAAAAAAATGAAAGTATTCCTTTTATCTTCTTTTAGAAGTAATTTTATTTGAGGAATATAGTGGTAATATTTTTAAATTTCCAGAAATAGAATTTAAATATACTATGAATATTATAACATATTACTTCTTATATTTGCTTTAAAATGTTTAAAAATAAGACCAATAGTCAACATGTTGCCAAAACTCTATTTTGACTTCCAATAATATAGCATTGTTTCTTTGTCATTTTCTGTTTTTGATGAAAAACAG
Seq C2 exon
ATAATCTGATATTTCATATGAAAAATGAAATCAGAAGCACCAAGTATAAACCAGTAGACTACCAACAATTGCATGCATTAACTGAAGCAAAAAAGTTAGCTTCTGCCTCTACAGAGCTAAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000004267:ENSBTAT00000005591:2
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion (1st CDS intron)
No structure available
Features
Disorder rate (Iupred):
C1=0.111 A=NA C2=0.024
Domain overlap (PFAM):
C1:
PF0070316=Glyco_hydro_2=PU(13.1=77.8)
A:
NA
C2:
PF0070316=Glyco_hydro_2=FE(37.4=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Human
(hg38)
No conservation detected
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGGAAAACATGAATAAAAGAGACCA
R:
CTTTAGCTCTGTAGAGGCAGAAGC
Band lengths:
182-1563
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]