BtaINT0072360 @ bosTau6
Intron Retention
Gene
ENSBTAG00000033398 | HKDC1
Description
hexokinase domain containing 1 [Source:HGNC Symbol;Acc:HGNC:23302]
Coordinates
chr28:25774612-25776091:+
Coord C1 exon
chr28:25774612-25774767
Coord A exon
chr28:25774768-25775857
Coord C2 exon
chr28:25775858-25776091
Length
1090 bp
Sequences
Splice sites
5' ss Seq
GAGGTGAGC
5' ss Score
8.7
3' ss Seq
CATTCCTGTCCCCTGGCCAGGTA
3' ss Score
8.46
Exon sequences
Seq C1 exon
GTTTGAAAAGATGATCAGCGGCCTATACTTGGGTGAACTCGTCAGGCTCATCTTGCTGAAGATGGCCAAGGCAGGCCTCCTGTTTGGCGGTGAGAAATCCTCTGCTCTTCACATTAAGGGCAAGATTGAAACACGGCATGTGGCTGCCATGGAGAG
Seq A exon
GTGAGCTACTGGCAGGGTCCCTCCCACCTTGACTCTGAAGAGGGTAAGGTGGGCAGCCAAGGCATGGTGTGCTCATGGGAGTGGTGCATAGGAGGAACCAGATCTTCTTCTTCACCAGGACGATTTCAGACCTGTGCACAGCTTCAGGTGGATTAGCAGTGCCTGTCATGCCTCACATGTGGCTTCTGGTTGGTTTCAATAGCCAGGTTTTCTGCTGTTGGAGGTTTACCCCCTTCTTATTTTGCCTTTTTCCTCCCTAAACTGGTGATATAACCTTTTTAGGGTTGCAGATCCCTTTGAGAGTCTAAGTTCTGGACCCTTTACCCCCAAAATGCACACTTGCACACAATTCTGCTTATAATATCAGGGCATCTTTGGAACCATTAAGGCCCTAGCGTGGAATCCAAGTTAAGAATTCCCACTACAGCAATAATCTTCTCTACCTGCTGCTGTCAGGGCCTGCATTTGGCTTTGGCTTTGGTGATTAGAGAGAAGAGCTCACACAAAGGTCTGCAGTAGGGAGCTGACTGCTGAGGCTGACTGGCCCCGGTTGACTCAAGATCCTGTTAGAAACCCTGGCACTGCCCTGAGCTTTGCAATCAGCTTAGATTCTGAGCTCAGCAGGGTGACATCTGACTTCCAGAATCTGTTTATGCAGAGCTTGGCAGGGACTGAGTTTCTATGAAAGGCAGTGCAGTATAGGGTGCTTACAAGGGTGGGCTCCTTAATCTAACTGCCGGAGTTCAAGTCTTGACCCTTGTACTCACCAGCCGTGTGCCATGGACAGGTTACCTACCCTGTCTGTGCTTCAGATCCCTCTTCAGCACAGTAGGATAATGACGGTCCTCCCCCCAGGTTTGTTGTGAGGACTAAGTTAACACACATGTCAAGTCCTTAGACTAGCACCCGGCCCATAGTGAGTGAGCAATAAGTGGTGGCCGCCATGATCATTGTTGAATCATCATTATTATTATAAGATGATGTGCCAGCCTGTTTATGGTCACAGTCCCGAGAGGTGGGAACACTCTCTCTGAGGGGGATTCCCATGGGATGGGGCAGTGTGATCCTCTCATTCCTGTCCCCTGGCCAG
Seq C2 exon
GTACAAAGAAGGCCTAGCCAATACGAGAGAGATTCTGACAGATTTGGGCCTGGAGCCGTCCGATGCAGACTGCATTGCGGTCCAGCACGTCTGCACCATCGTCTCCTTCCGCTCGGCCAATCTCTGCGCAGCCGCCCTGGCAGCCATCCTGACACGCCTCCGGGAGAACAAGAAGCTGGTGCGGCTCCGCACCACGGTGGGCGTGGACGGCACACTCTACAAGATTCACCCCCA
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000033398:ENSBTAT00000047474:8
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0372711=Hexokinase_2=FE(21.7=100)
A:
NA
C2:
PF0372711=Hexokinase_2=FE(32.5=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CTCGTCAGGCTCATCTTGCTG
R:
CTTGTAGAGTGTGCCGTCCAC
Band lengths:
342-1432
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]