BtaINT0092123 @ bosTau6
Intron Retention
Gene
ENSBTAG00000003300 | MFGE8
Description
milk fat globule-EGF factor 8 protein [Source:HGNC Symbol;Acc:HGNC:7036]
Coordinates
chr21:20890804-20892176:-
Coord C1 exon
chr21:20891992-20892176
Coord A exon
chr21:20890960-20891991
Coord C2 exon
chr21:20890804-20890959
Length
1032 bp
Sequences
Splice sites
5' ss Seq
CAGGTGGGT
5' ss Score
8.56
3' ss Seq
TTTTTTTTCACCCCTTGTAGATT
3' ss Score
10.24
Exon sequences
Seq C1 exon
GATGCACTGAACCCCTAGGCCTGAAGGATAATACCATCCCCAACAAGCAGATCACAGCCTCCAGCTACTACAAAACCTGGGGCCTGAGTGCCTTTAGCTGGTTTCCCTACTACGCACGACTGGATAATCAGGGCAAGTTCAACGCCTGGACCGCCCAGACCAACAGTGCCTCTGAGTGGCTGCAG
Seq A exon
GTGGGTCAGGCTCCTCCGGGGATGCAGGGTTGGGGTTGGGTGGGGGTGTGGGATCTATGACCTTGGACCAACCCTGGCTTCTGCTTGCCAAGAGGGAGTTTCTTTGAGCCTTGTTTGTGTTCGACTTTGCTCTTTTTTTCCTCTTGTGTTACCACCTTTCTGCTGATTCTGTCACTCAAGGCTGGGGGGATGGTGGAGGAGCTCAGAGCTGCTTCCGTCAAGGCCCTCGGCCCCAGGTGATGAGTGTGTGTGTGTGTAGAGGTGGGGCCACCTCCATTGGCTGCCACAGGGTAAGGCTGAGCCCAGGGACAGCAGGGCAGGATGGAGGAGGGATGTGTCGGGGTTTCTGTGCCTGCTGTCAGAGGATGCGCTGTAACTCACCGAGAGCACACCGTGCGACAGCCCTTGGGTTTCACTGCAGTTGGGAAGCTCAGGAGAGTCGTGGAAAACCAGAGCATCCTCTTATCTCTGAGGAAGCCGGGTCCTGGCAGGGCAAGGACTTGCCTGGGGCTCCCCTTCTGGTTGGCAGTGAGATGGGTTTGTAGCCTGGATCTTACTGAGCACCGTCTTCCCCTGGTCAGCTGGCAGGCTCCCCAGGATGGACTGGGGGGCAGGAGGCTGGTCGTGTCTCTAGGCCCCAAGGAGCACATTAGCCTGGGGGACTGGGTTCCATGAGAAGCTGCCTGCCTTTGGAATCCCCCAGGACCAATGTCAGGCCCTGGACAGCCTGCAGGGGCCTCATGAGCAGTTTATGTCCCTATAGACTATGGCCTCTCTGCCCCTGTTCCCGTTGCACAAGGGTTGCCTGGGACCAGGCTCTGAAATCTGACATTGCAGTGAAGTGCTTGACAAAGTTGCTGGGATTATTTATGCCCACTTGATTGATGGGTAAACTGAGGCTCTGCCAAATGAAGTAATTTTCCCGAAACTAGAATGTATGCCCCAGCGTGTATGTGTGCTTTTCCCCACCTTACTGGGAATGGTGCAAGCAAAGAAGACGGGATTGGTTTTTTTTTTTTTCACCCCTTGTAG
Seq C2 exon
ATTGACCTGGGCTCCCAGAAGCGAGTCACGGGCATCATCACCCAGGGTGCCCGAGACTTTGGCCACATTCAATATGTGGCTGCCTACAGGGTGGCCTATGGTGATGATGGTGTGACCTGGACTGAGTACAAGGACCCGGGGGCCTCAGAAAGCAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000003300:ENSBTAT00000004272:7
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.038
Domain overlap (PFAM):
C1:
PF0075420=F5_F8_type_C=PU(32.1=72.6)
A:
NA
C2:
PF0075420=F5_F8_type_C=FE(36.4=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ACTGAACCCCTAGGCCTGAAG
R:
CTTGCTTTCTGAGGCCCCC
Band lengths:
336-1368
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]