Special

DmeALTD0000294-2/2 @ dm6

Alternative 5'ss

Gene
Description
The gene Arrowhead is referred to in FlyBase by the symbol DmelAwh (CG1072, FBgn0013751). It is a protein_coding_gene from Dmel. It has 5 annotated transcripts and 5 polypeptides (4 unique). Gene sequence location is 3L:3846206..3861692. Its molecular function is described by: RNA polymerase II regulatory region sequence-specific DNA binding; DNA-binding transcription factor activity; DNA-binding transcription factor activity, RNA polymerase II-specific. It is involved in the biological process described with 6 unique terms, many of which group under: developmental process; cellular nitrogen compound biosynthetic process; multicellular organismal process; heterocycle metabolic process; nucleobase-containing compound biosynthetic process. 44 alleles are reported. The phenotypes of these alleles manifest in: larval thorax; imaginal ring; tracheal system; polytene chromosome puff; pupal spiracle. The phenotypic classes of alleles include: increased mortality during development; fertile; increased mortality; phenotype. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderate expression to a trough of extremely low expression. Peak expression observed within 06-18 hour embryonic stages.
Coordinates
chr3L:3860190-3860761:+
Coord C1 exon
chr3L:3860190-3860411
Coord A exon
chr3L:3860412-3860423
Coord C2 exon
chr3L:3860636-3860761
Length
12 bp
Sequences
Splice sites
5' ss Seq
AAAGTAAGT
5' ss Score
9.72
3' ss Seq
CAAACGATTTTTGATTGCAGTAC
3' ss Score
3.52
Exon sequences
Seq C1 exon
AGGGCTGTGATGGCGATGGTTATCACAAGAGCAAGACGAAACGGGTGCGCACCACCTTCACCGAGGAGCAGTTGCAAGTGCTGCAGGCCAACTTCCAGATTGACAGTAATCCGGATGGCCAGGATCTGGAGCGGATCGCCTCGGTGACCGGTCTCAGCAAGCGTGTGACGCAAGTATGGTTCCAGAACTCGCGGGCGCGTCAGAAAAAACACATACATGCTG
Seq A exon
GTAAGAATAAAA
Seq C2 exon
TACGCGAACCGGAAGGAAGCTCATTTGCGCGTCATATTAACCTGCAGTTAACGTATTCATTTCAGAATAACGCACAGAACCCAATGCATTTGAATGGCTCTAAAGCGGGTCTATACCCGACTCATG
VastDB Features
Vast-tools module Information
Secondary ID
FBgn0013751-4-4,5-4-2/2
Average complexity
Alt5
Mappability confidence:
NA
Protein Impact

Protein isoform when splice site is used (Ref)

No structure available
Features
Disorder rate (Iupred):
  C1=0.269 A=NA C2=0.698
Domain overlap (PFAM):

C1:
PF0004624=Homeobox=WD(100=76.0)
A:
NA
C2:
NO


Main Inclusion Isoform:
FBpp0303103


Main Skipping Isoform:
FBpp0303104


Other Inclusion Isoforms:
FBpp0073013, FBpp0073014


Other Skipping Isoforms:
NA
Associated events
Conservation
Human
(hg38)
No conservation detected
Human
(hg19)
No conservation detected
Mouse
(mm10)
No conservation detected
Mouse
(mm9)
No conservation detected
Rat
(rn6)
No conservation detected
Cow
(bosTau6)
No conservation detected
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GTATGGTTCCAGAACTCGCGG
R:
TGCAGGTTAATATGACGCGCA
Band lengths:
95-107
Functional annotations
There are 0 annotated functions for this event


GENOMIC CONTEXT[edit]

INCLUSION PATTERN[edit]


SPECIAL DATASETS

  • Neural diversity
  • Neurogenesis
  • Neuronal activity
  • Splicing factor regulation (brain)
  • Splicing factor regulation (SL2)