DmeEX0001301 @ dm6
Exon Skipping
Gene
FBgn0039463 | CG18472
Description
The gene Spag1 axonemal dynein assembly factor is referred to in FlyBase by the symbol DmelSpag1 (CG18472, FBgn0039463). It is a protein_coding_gene from Dmel. It has 2 annotated transcripts and 2 polypeptides (1 unique). Gene sequence location is 3R:26947830..26949682. Its molecular function is described by: heat shock protein binding. It is involved in the biological process described with: protein targeting to mitochondrion. 6 alleles are reported. No phenotypic data is available. The phenotypic class of alleles includes: viable. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of no expression detected. Peak expression observed at stages throughout the pupal period, in adult male stages.
Coordinates
chr3R:26948249-26949038:+
Coord C1 exon
chr3R:26948249-26948667
Coord A exon
chr3R:26948724-26948851
Coord C2 exon
chr3R:26948911-26949038
Length
128 bp
Sequences
Splice sites
3' ss Seq
TATTTTTGTTGTTGTTAAAGACA
3' ss Score
3.23
5' ss Seq
CTCGTAAGT
5' ss Score
9.63
Exon sequences
Seq C1 exon
GATTGGACTGACGCCATAAAAACAAAGGACAATGCTCTCAATGAGCTGAAGAAAGTTAAGCAGAATTTGAATCTTCCATCTGTGCGTAAACTTTCGAAAATCGATTTGGAGAAGGAAAGTAAAACTGAAAAACCCAAGCCAGCGCCAAAGGCTACATCTCCGTCCAATACCAAGAATAAGGAGGCTCGAATCAAATCCACCGATTACAGAAAATGGGATAAGTACGATCCCGATGAGGAGATACTGCGCATGGATTTGAACGAGGAACGCGACCAGGAGCAAAGAGAGAAAATCATTTCCAATCACAGTAAATCGGTTACCACGGATAAGCTACAGAGCGAAAGAGATTCTCTATATGAACGCCTCCAAGCACAGCTAAAGAACCTAAGCCAGCTGGAGAAGGAGCAATTTGCTGAAAG
Seq A exon
ACATCGACTGCGTGGCAATGAGAGCTTCAAGGCAAAGGAATATGAAAATGCCATTGAGGAGTACAACTGTTCAATAATATACGATCCGGAGAATGCAGTACACGCTTACAATAATCGCGCTGTCGCTC
Seq C2 exon
ATCTCAAGTTGAAGAAATACTTTTCGGCCATATCGGACTGCCAAGCCTGTCTGCAGATCGATCCAATGAATATTAAGGCACACCTTCGAATGGCTGAGGCCCACAATGCCGAAGGAAAACATCTTGAG
VastDB Features
Vast-tools module Information
Secondary ID
FBgn0039463-'1-2,'1-1,2-2
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.650 A=0.114 C2=0.163
Domain overlap (PFAM):
C1:
PF149701=DUF4509=PU(79.9=96.4),PF134141=TPR_11=PU(5.8=2.9)
A:
PF149701=DUF4509=PD(19.5=75.0),PF134141=TPR_11=FE(62.3=100)
C2:
PF134141=TPR_11=PD(30.4=48.8),PF134141=TPR_11=PU(37.7=46.5)
Main Inclusion Isoform:
FBpp0084440
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Human
(hg38)
No conservation detected
Human
(hg19)
No conservation detected
Mouse
(mm10)
No conservation detected
Mouse
(mm9)
No conservation detected
Rat
(rn6)
No conservation detected
Cow
(bosTau6)
No conservation detected
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGAGGAGATACTGCGCATGGA
R:
ATCGATCTGCAGACAGGCTTG
Band lengths:
248-376
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Neural diversity
- Neurogenesis
- Neuronal activity
- Splicing factor regulation (brain)
- Splicing factor regulation (SL2)