Special

DmeEX0002443 @ dm6

Exon Skipping

Gene
FBgn0261570 | CG42684
Description
The gene raskol is referred to in FlyBase by the symbol Dmel askol (CG42684, FBgn0261570). It is a protein_coding_gene from Dmel. It has 10 annotated transcripts and 10 polypeptides (9 unique). Gene sequence location is X:17710144..17752007. Its molecular function is unknown. It is involved in the biological process described with: negative regulation of Ras protein signal transduction; regulation of GTPase activity. 37 alleles are reported. The phenotypes of these alleles manifest in: border follicle cell; filamentous actin. The phenotypic classes of alleles include: cell adhesion defective; viable; behavior defective; smell perception defective; fertile; cell migration defective. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 06-12 hour embryonic stages.
Coordinates
chrX:17721423-17746691:+
Coord C1 exon
chrX:17721423-17721615
Coord A exon
chrX:17736198-17736269
Coord C2 exon
chrX:17746571-17746691
Length
72 bp
Sequences
Splice sites
3' ss Seq
ATCTTTTCCACTACTTACAGGTG
3' ss Score
9.35
5' ss Seq
AAGGTAACA
5' ss Score
8.92
Exon sequences
Seq C1 exon
CTAGGCGATGTTTGCTACGAGGACATTCCAAGGGTTCGGTTGGACCGAGTTCGTCGTCCTGCGAAGGCTTTGTGGGACGGACCGCCGACGTTGGTCGAGGAAAACGAGGATTCAGATTCGTGTGTTGGGGGCAGCGGCGGCATGAGTGGCATCAACGACATCGTCCTGAATACGACGCTCTACAGTGAACTAG
Seq A exon
GTGAGTACAAGTCGAAGACACTGCCTCGCATACATTTCGATACGGCGTTAAACGATACATCGCTGAATGAAG
Seq C2 exon
ATACGTCCTATGAGAAGGCGTGCCGCCGTGGATCAGCGCCCACCACGCCCATTTTGGGCAGCAAACAGCACCAGACGGAGCACAATGCCACCTCGCGTTTCACCAACTTCTTTTCCAAGAA
VastDB Features
Vast-tools module Information
Secondary ID
FBgn0261570_CASSETTE2
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact





	

	
Alternative protein isoforms (No Ref)





No structure available

		

			
Features


			

				
Disorder rate (Iupred):
  C1=0.233 A=0.520 C2=0.696
 
                        

				
Domain overlap (PFAM):

					





						

							
C1:
NO

							
A:
NO

							
C2:
NO

						

					

				








    
Main Inclusion Isoform:
FBpp0291977





     
                   









    
Main Skipping Isoform:
FBpp0309494





     
      









    
Other Inclusion Isoforms:
FBpp0291975, FBpp0291976, FBpp0309493
          









    
Other Skipping Isoforms:
FBpp0291974, FBpp0309495
  



Associated events







Conservation


Human
(hg38)
No conservation detected
Human
(hg19)
No conservation detected
Mouse
(mm10)
No conservation detected
Mouse
(mm9)
No conservation detected
Rat
(rn6)
No conservation detected
Cow
(bosTau6)
No conservation detected
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected




Primers PCR

	

		

			
Suggestions for RT-PCR validation

			

				
F: 
ACGTTGGTCGAGGAAAACGAG

				
R: 
GTGCTGTTTGCTGCCCAAAAT

				
Band lengths: 
177-249

			

		

	





	
Functional annotations
(Submit an annotation)

	


There are 0 annotated functions for this event 


		










GENOMIC CONTEXT[edit]












INCLUSION PATTERN[edit]






SPECIAL DATASETS
  • Neural diversity
  • Neurogenesis
  • Neuronal activity
  • Splicing factor regulation (brain)
  • Splicing factor regulation (SL2)





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