DmeEX0007553 @ dm6
Exon Skipping
Gene
FBgn0020309 | crol
Description
The gene crooked legs is referred to in FlyBase by the symbol Dmelcrol (CG14938, FBgn0020309). It is a protein_coding_gene from Dmel. It has 10 annotated transcripts and 10 polypeptides (7 unique). Gene sequence location is 2L:11794327..11809438. Its molecular function is described by: nucleic acid binding. It is involved in the biological process described with 8 unique terms, many of which group under: imaginal disc-derived appendage development; regulation of cell communication; signal transduction; instar larval or pupal morphogenesis; cell adhesion. 61 alleles are reported. The phenotypes of these alleles manifest in: external sensillum; external sensory organ; mechanosensory sensory organ; embryonic/larval peripheral nervous system; pupa. The phenotypic classes of alleles include: increased mortality; phenotype; some die during P-stage; viable. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 06-18 hour embryonic stages, during early pupal stages.
Coordinates
chr2L:11800155-11803898:-
Coord C1 exon
chr2L:11803785-11803898
Coord A exon
chr2L:11802905-11803674
Coord C2 exon
chr2L:11800155-11800322
Length
770 bp
Sequences
Splice sites
3' ss Seq
GCATTTTTTGTTTGTTGTAGCCA
3' ss Score
8.49
5' ss Seq
CGGGTGAGT
5' ss Score
9.89
Exon sequences
Seq C1 exon
GTGAACAATACAAATTGGTTGTGCGCCAATTAAACACCCAAATGGATCAACTGTTACAGTAGCAGCCACAACATCAGCACAACGGGAGCCAGAGCAAGAGGCAGCAATTTCATG
Seq A exon
CCACGCTCAGCGAAAGCAGTCGACAGCAGCAACAACAGCAATATGTAGGCGCTCCTCAGCAGCAACACATGAAATACAACAGCCAAGAACAGCAGCAACATCAACAACAACTACTCCCAACAAACCAATAACTCCCAAAACAAAAAAAAAACAACACAACAACAAGAACATCATTAGGATATAGGAATCAGGGAACGAGCAACGCCACAACGATCGCCCGGATCTGGACACAAGAATGCAGCACGTGAGCGCTGCCAGCTCGGTGCCATCAGTAGTAACTCCTGTTGTGACCACTGGTGGGACAACGATCACATTGGGCGGCCCACCACCGCTCCCTAAATCCGAGCACAAAGAGGATGGCAAGCCGCCGCACGGCATCGAAATGTACAAGGTGAACATTGAGGACATTTCGCAGCTCTTCACCTACCACGAGGTCTTTGGCAAAATCCACGGCGATGTGGTCAATCATCAATTAGCAGCGGCCCACGGCGGCCAGTTGCCACCACCTCCTCCGCTGCCGCCGCAGGTTACCAGTCATGCGGCGAGTGCAGCGGCAGCCGCAGCAGCGGCGTCCACGAATAATGCCGCCGTTGCAGCGGTAATGGCATCAGCGAATGCAGCAGCGGCCGCGGCGGCAGCTGCATCGGCGGGGGGAGGACTACCGCCGGCCACCAGCGGCAATGGGGGCCAGCAGGTGACGGTGACGACGACCAGCAGCTCGACTAGCAGCGGCGGGAGCACCACCAGTGGGGGCACCACGACCACGGCGG
Seq C2 exon
GTCAAACGCCGCACCAGTGCGATGTCTGCGGCAAGAAATATACGCGCAAGGAGCACCTAGCCAATCATATGCGATCCCATACCAACGAGACGCCGTTCCGTTGCGAGATCTGCGGCAAGAGCTTTAGCCGCAAGGAGCACTTCACCAATCACATACTTTGGCATACAG
VastDB Features
Vast-tools module Information
Secondary ID
FBgn0020309_MULTIEX1-1/3=C1-2
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref, Alt. ATG (>10 exons))
No structure available
Features
Disorder rate (Iupred):
C1=NA A=0.511 C2=0.135
Domain overlap (PFAM):
C1:
NA
A:
PF134651=zf-H2C2_2=PU(23.8=2.8)
C2:
PF0013017=C1_1=PD(30.8=28.1),PF134651=zf-H2C2_2=PD(57.7=26.3),PF134651=zf-H2C2_2=WD(100=45.6),PF134651=zf-H2C2_2=PU(38.5=17.5)
Main Inclusion Isoform:
FBpp0079847
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
FBpp0079845, FBpp0079846, FBpp0293786, FBpp0307448, FBpp0307450, FBpp0307451
Other Skipping Isoforms:
NA
Associated events
Conservation
Human
(hg38)
No conservation detected
Human
(hg19)
No conservation detected
Mouse
(mm10)
No conservation detected
Mouse
(mm9)
No conservation detected
Rat
(rn6)
No conservation detected
Cow
(bosTau6)
No conservation detected
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CAAATTGGTTGTGCGCCA
R:
TGTATGCCAAAGTATGTGATTGGTGA
Band lengths:
271-1041
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Neural diversity
- Neurogenesis
- Neuronal activity
- Splicing factor regulation (brain)
- Splicing factor regulation (SL2)