DmeEX6024554 @ dm6
Exon Skipping
Gene
FBgn0051004 | mesh
Description
The gene mesh is referred to in FlyBase by the symbol Dmelmesh (CG31004, FBgn0051004). It is a protein_coding_gene from Dmel. It has 6 annotated transcripts and 6 polypeptides (3 unique). Gene sequence location is 3R:31178389..31195762. Its molecular function is described by: protein binding. It is involved in the biological process described with: smooth septate junction assembly; cell-matrix adhesion. 6 alleles are reported. The phenotypes of these alleles manifest in: smooth septate junction; embryonic/larval midgut. The phenotypic classes of alleles include: viable; lethal - all die before end of first instar larval stage; some die during pupal stage; partially lethal - majority die; some die during first instar larval stage; short lived. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of very low expression. Peak expression observed within 12-24 hour embryonic stages.
Coordinates
chr3R:31179583-31180818:-
Coord C1 exon
chr3R:31180579-31180818
Coord A exon
chr3R:31180459-31180509
Coord C2 exon
chr3R:31179583-31180389
Length
51 bp
Sequences
Splice sites
3' ss Seq
ACCGCAAATTGATTTTCCAGCTA
3' ss Score
6.02
5' ss Seq
AAGGTAGTC
5' ss Score
5.64
Exon sequences
Seq C1 exon
GTGAGGTGTACTACACGCGTCGCGTCGCTTTCATAGCAATTGGCATTATCTTCCTGGTGATCTGCCCACTGATGCTGTGCATCGTGTGTGGAGTATATCGCTATCGCCAGAAGCAGCTCAAGGAGGATCCGCAGTGGCAGATGCCCATGCTGCCCCGTTCGAGGGCTAGTTCGGCTCGTAATTTAAGGACACTTAACTACGACGACGACAGCGATACGGATGCCAGCACATTGAAGAAAA
Seq A exon
CTAAGAAATGGGATCTGGATGAGAACGACGAGGATGTGACCTCATCGGAAG
Seq C2 exon
GTGAGAAGCCGAAACAGCTGGGTCGCCGCTCCTTGCACTCCGCCTCCGGAACGGAGTTGGATCAGCATGGCTCCCCGGTGGACGGGGATCATCCAGACGAGGACGATGACTTCGACGAGGCGGATGTCCACACGGGCACCACTCATCCAGTCGGATACCACCAGCCCCTGTCGCCGGAGGAGGCGGATCAGCTGCATCGCCAGCAGTACAGCCCCACCTTCAGTGGCCTGGACAGTCGCACCAGTGGCGCCAGTTCCATAAACTACACCCCACAGGCGGCGACGCAGAACCGCTTCGGAGGAGTGCCGGTGCTGCCTAGCAACACCCAGTACTACAGTAGACCCCCGTCGAGCGTGAACACCGTGCCGCTCCGTACTGCGCCCACGCCCCTGACGCAGGACACTGGTCTGCCTTCGCCTCCCCTGGCCACCTCGCCCACTCCCTCGGTGCAGAAGTCCACGGAGGTCTGAGGATCGGCACTCAACCCGATCCTTAAGCTG
VastDB Features
Vast-tools module Information
Secondary ID
FBgn0051004-'14-17,'14-16,15-17=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.367 A=0.738 C2=1.000
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
NO
Main Inclusion Isoform:
FBpp0085140
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
FBpp0085141, FBpp0290183
Other Skipping Isoforms:
NA
Associated events
Conservation
Human
(hg38)
No conservation detected
Human
(hg19)
No conservation detected
Mouse
(mm10)
No conservation detected
Mouse
(mm9)
No conservation detected
Rat
(rn6)
No conservation detected
Cow
(bosTau6)
No conservation detected
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CGATACGGATGCCAGCACATT
R:
TCGTCCTCGTCTGGATGATCC
Band lengths:
135-186
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Neural diversity
- Neurogenesis
- Neuronal activity
- Splicing factor regulation (brain)
- Splicing factor regulation (SL2)