DmeINT0000575 @ dm6
Intron Retention
Gene
FBgn0037555 | Ada2b
Description
The gene transcriptional Adaptor 2b is referred to in FlyBase by the symbol DmelAda2b (CG9638, FBgn0037555). It is a protein_coding_gene from Dmel. It has 4 annotated transcripts and 4 polypeptides (2 unique). Gene sequence location is 3R:8236632..8240515. Its molecular function is described by: transcription coactivator activity; DNA binding; chromatin binding; protein binding; zinc ion binding. It is involved in the biological process described with 7 unique terms, many of which group under: histone acetylation; chromatin remodeling; multicellular organismal process; positive regulation of protein modification process; transcription by RNA polymerase II. 30 alleles are reported. The phenotypes of these alleles manifest in: glial cell; trichogen cell; wing disc. The phenotypic classes of alleles include: viable; lethal; increased mortality during development; phenotype. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of moderate expression. Peak expression observed within 00-06 hour embryonic stages.
Coordinates
chr3R:8238103-8239606:-
Coord C1 exon
chr3R:8238731-8239606
Coord A exon
chr3R:8238253-8238730
Coord C2 exon
chr3R:8238103-8238252
Length
478 bp
Sequences
Splice sites
5' ss Seq
ACCGTAAGT
5' ss Score
10.43
3' ss Seq
TCTTGTCTCACGATATTCAGCTC
3' ss Score
4.05
Exon sequences
Seq C1 exon
TGCTTTGCCGCAGGTGCGGAGATCGGCGCCCATCAAAACAACCACTCCTACCAGTTCATGGACACCGGCACTTCCATATTGAGTGTATTTCGTGGAAAGGGGGCGTGGACGGCACGAGAGGAAATCCGCCTGTTGGATGCAATCGAGCAGTACGGCTTTGGAAACTGGGAGGACATTAGCAAGCACATTGAGACGAAGTCAGCGGAGGATGCCAAAGAGGAGTACGTGAATAAGTTTGTAAATGGCACGATTGGAAGGGCCACTTGGACGCCGGCCCAGAGCCAGAGACCCCGCCTTATCGATCACACTGGAGACGACGACGCTGGTCCTCTGGGCACCAATGCTCTCTCTACCTTGCCTCCACTGGAAATCAACTCCGATGAGGCCATGCAGCTGGGCTACATGCCCAACAGGGACAGCTTTGAGCGGGAGTACGATCCCACGGCAGAGCAACTGATCTCGAACATCTCATTAAGCTCCGAGGATACGGAGGTGGATGTGATGCTTAAGCTGGCCCACGTTGACATATACACCCGCCGACTCAGAGAGCGTGCTCGCAGGAAACGGATGGTCAGGGACTATCAATTAGTATCAAATTTCTTCCGAAACAGAAATTACGCTCAGCAACAAGGACTCACCAAGGAGCAGCGCGAGTTTCGGGACAGATTTCGGGTCTACGCCCAGTTCTACACGTGTAACGAGTATGAGCGGCTATTGGGATCACTGGAGCGCGAAAAGGAACTGCGCATTCGACAGTCAGAGCTGTACCGATACCGGTATAATGGCCTGACCAAAATCGCGGAATGCACACATTTCGAGCAGCACGCCGCAACAGCGACGCACCGCTCTACAGGACCATATGGCCATGGCAAGACC
Seq A exon
GTAAGTTTAAGGCTCCGCAACAGGGGTGCCGAAATCGAACTAACCATTAAATATTTTATTTGGTCTTGAAAATCCGAACTTTTTGGAACTTTGTTATTTTTTTTTTTATTATTAAAATCCAAACATTACCTTTTCTTTCGATTGTTTCACAAAAATTAAAAGAGGTGTTAGGTGACCGGAACCGGTAGCTTTATAATTTTATCAACAATGAGTAGATTAACTTAAATTGAAAGAAAACAAATGATTTAAGTTGAATGTATAGATAAAAGTTTGGATAAATCGATTTGGAATACTTAATACAGAATATTAAATGGTCAAATTGGCTAGTTTCGATTTCATTCCCCTTGGAGTAAGCTTTTCCCACTGAGAATGCATGACGAGTTGAATATCGTGTGTTCTGCCTTGCTTTAACCGTCGTCCTTACCTGAACTGACCCAACTCTTACAATCGCTTTTCCGTCTTGTCTCACGATATTCAG
Seq C2 exon
CTCCACTGCATAATAAACGCGCCAAACAACGAGCGTTCGAATGGTTCTATAAGACAATGTTTAACCACAGGACCACACGCATACCTCCAATGGAAGTCATCGGCCGCCAAGCTCTTCCTTGCACTCCCCACAACCGAATCTCAGAAAGGT
VastDB Features
Vast-tools module Information
Secondary ID
FBgn0037555:FBtr0081807:2
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.220 A=NA C2=0.321
Domain overlap (PFAM):
C1:
PF0056912=ZZ=PD(27.3=4.1),PF0024926=Myb_DNA-binding=WD(100=15.4)
A:
NA
C2:
NO
Main Inclusion Isoform:
NA
Main Skipping Isoform:
FBpp0081303
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
FBpp0099776, FBpp0306430
Associated events
Conservation
Human
(hg38)
No conservation detected
Human
(hg19)
No conservation detected
Mouse
(mm10)
No conservation detected
Mouse
(mm9)
No conservation detected
Rat
(rn6)
No conservation detected
Cow
(bosTau6)
No conservation detected
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TCTACGCCCAGTTCTACACGT
R:
TGAGATTCGGTTGTGGGGAGT
Band lengths:
346-824
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Neural diversity
- Neurogenesis
- Neuronal activity
- Splicing factor regulation (brain)
- Splicing factor regulation (SL2)