DmeINT0000746 @ dm6
Intron Retention
Gene
FBgn0011747 | Ank
Description
The gene Ankyrin is referred to in FlyBase by the symbol DmelAnk (CG1651, FBgn0011747). It is a protein_coding_gene from Dmel. It has 5 annotated transcripts and 5 polypeptides (1 unique). Gene sequence location is 4:115074..129849. Its molecular function is described by: cytoskeletal anchor activity; spectrin binding; ion channel binding. It is involved in the biological process described with: signal transduction; protein localization to plasma membrane. 10 alleles are reported. The phenotype of these alleles manifest in: ovariole. The phenotypic class of alleles includes: viable. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderately high expression. Peak expression observed at stages throughout embryogenesis, during late larval stages, at stages throughout the pupal period.
Coordinates
chr4:120858-121956:-
Coord C1 exon
chr4:121853-121956
Coord A exon
chr4:121800-121852
Coord C2 exon
chr4:120858-121799
Length
53 bp
Sequences
Splice sites
5' ss Seq
CAGGTATTG
5' ss Score
8.35
3' ss Seq
AATAAATTTATTTATTTTAGGCG
3' ss Score
7.2
Exon sequences
Seq C1 exon
GATGTATAAATATTGTAATATATTTACTACAACATGAGGCTAGTGCGGATTTACCAACAATACGCGGAGAAACACCACTTCACTTGGCAGCTCGTGCTAATCAG
Seq A exon
GTATTGCTTTAAGTATATAGCATAAAATATATTAATAAATTTATTTATTTTAG
Seq C2 exon
GCGGATATTATTCGAATTTTACTTCGCAGCGCAAAAGTGGATGCTATTGCCCGTGAAGGCCAAACGCCACTTCATGTAGCTTCTCGATTAGGAAATATTAATATAATTATGTTATTACTGCAGCATGGAGCAGAGATAAATGCTCAATCTAATGACAAATATTCGGCGCTGCATATTGCTGCAAAAGAAGGACAAGAGAATATAGTCCAAGTGTTACTGGAAAATGGTGCGGAAAATAATGCTGTTACCAAAAAAGGGTTTACTCCACTGCATTTAGCTTGCAAATACGGAAAGCAGAATGTGGTTCAAATATTACTACAAAATGGAGCTTCTATTGATTTTCAGGGGAAAAATGATGTGACCCCATTACACGTGGCCACGCACTACAATAATCCCTCAATAGTAGAACTCCTTTTAAAAAATGGGTCATCGCCAAATTTATGTGCCCGAAATGGTCAATGTGCTATTCACATAGCTTGCAAAAAAAATTATCTGGAAATTGCGATGCAGCTGTTGCAACACGGTGCAGACGTTAATATTATTAGCAAGAGTGGCTTCTCTCCGTTACATTTGGCGGCTCAAGGGGGAAACGTTGATATGGTCCAACTACTTCTAGAGTATGGAGTTATTAGCGCCGCTGCAAAAAATGGCCTAACACCATTACATGTGGCTGCCCAGGAGGGCCATGTTCTTGTATCACAGATTTTACTGGAGCACGGTGCTAACATTTCAGAGCGCACTAGGAATGGTTATACTCCCCTTCATATGGCTGCCCATTATGGTCACCTCGATCTTGTCAAGTTTTTTATTGAGAACGACGCTGACATTGAGATGTCTTCTAATATCGGGTATACCCCGCTTCATCAAGCCGCTCAGCAAGGTCACATTATGATTATTAATCTTTTATTACGCCATAAAGCGAATCCAAATGCTTTAACAAAA
VastDB Features
Vast-tools module Information
Secondary ID
FBgn0011747:FBtr0089173:4
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.003
Domain overlap (PFAM):
C1:
PF0002325=Ank=PD(60.6=57.1),PF136061=Ank_3=PU(43.3=37.1)
A:
NA
C2:
PF136061=Ank_3=PD(50.0=4.8),PF127962=Ank_2=WD(100=23.9),PF0002325=Ank=WD(100=10.5),PF127962=Ank_2=WD(100=29.9),PF0002325=Ank=WD(100=10.5),PF127962=Ank_2=PU(69.0=19.1)
Main Inclusion Isoform:
NA
Main Skipping Isoform:
FBpp0088238
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
FBpp0088239, FBpp0088240, FBpp0289724
Associated events
Conservation
Human
(hg38)
No conservation detected
Human
(hg19)
No conservation detected
Mouse
(mm10)
No conservation detected
Mouse
(mm9)
No conservation detected
Rat
(rn6)
No conservation detected
Cow
(bosTau6)
No conservation detected
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CAACATGAGGCTAGTGCGGAT
R:
TTCACGGGCAATAGCATCCAC
Band lengths:
132-185
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Neural diversity
- Neurogenesis
- Neuronal activity
- Splicing factor regulation (brain)
- Splicing factor regulation (SL2)