DmeINT0008760 @ dm6
Intron Retention
Gene
FBgn0267795 | CG32138
Description
The gene Formin-like is referred to in FlyBase by the symbol DmelFrl (CG32138, FBgn0267795). It is a protein_coding_gene from Dmel. It has 5 annotated transcripts and 5 polypeptides (4 unique). Gene sequence location is 3L:14053215..14071583. Its molecular function is described by: actin filament binding; GTPase binding; Rho GTPase binding. It is involved in the biological process described with 6 unique terms, many of which group under: pattern specification process; organelle organization; plasma membrane bounded cell projection organization; movement of cell or subcellular component; mushroom body development. 22 alleles are reported. The phenotypes of these alleles manifest in: material anatomical entity; lobe system of mushroom body; procephalic segment; ganglion; cell projection. The phenotypic classes of alleles include: planar polarity defective; decreased cell number; viable; lethal; visible; neuroanatomy defective.
Coordinates
chr3L:14068808-14070369:+
Coord C1 exon
chr3L:14068808-14068927
Coord A exon
chr3L:14068928-14069891
Coord C2 exon
chr3L:14069892-14070369
Length
964 bp
Sequences
Splice sites
5' ss Seq
CAGGTAAGT
5' ss Score
10.86
3' ss Seq
ACCAAACGAAATGCAAACAGTTG
3' ss Score
0.55
Exon sequences
Seq C1 exon
CAACACGATCAGGAGAACGAGCAGCGTCTTCGCCTGGAAAAGGCCGCTGCGCTGGCCGCTTCCAAGAAAGAGAACGATCAGGTGCTTATGCGCAACAAGGTTAACCAGAAGAAGCAACAG
Seq A exon
GTAAGTGGAGGACATTGACCACGAAGAGCTAGCTCCATCGAAGGGTGGCCAAGTCCCTCTAATCGGTTCCATATGTCTAATCTCTATATGCACGTATCGTTTCAAACCTGATAACCTGTTATGATTTGTTTTGATCTCGTTTGATTTGATTCATGCAGCAAATACATTGTCTGCTCGACTTTGCCAATCAGCAACAGAAGCAGCTTGCCCAATCGTAGCATCCACCCACCGGCCACTTCCTCAATCTAAGCACGTTAACTTGGTTATTTGGTTGACTCACACAAATCACATCACACTCTGTCTAATACCCAACTGGGGACGGGAATTCGAGGGGGACTTGGTCTGAGTTCGTGCCCCAAATCCCCGCTAATCCATCACAAAACAAAAAAAGAAATCAAAATCCTTGTTTGTTGCTCGTTGTGGGCCCCAATGTGACGGGATTTGTTTTGTATAAAGTTCCCACATTAGCGTAAGTCGCCCGGCTTGTCTACCTCCTAACCTTGTATTTGTTCAGACTAATCCTGCACTATTGTGTTTTCTAATCCCCGCAAAATTCCTCGTCAAATCCAGTCTACTTCGACCATTTGATTTAAATGTTGGATTCGTGCCAAGACTGCGCGATGCCAAATTTATTTATTTTGGAAATTATTTCAAATCTTTTGAAATATGAAAATTTAGAAATGTTGATAAAAAGGGAAATAAAATAGATAAATATTGAAGTTATACAATTTAACATTTGCCATATAATAACAGAATAAATTACTTTTAGATTCTTATAAAATTTGGAAAATCCCCGATAAAAAAAAATTGGCACTACAAAATATTAATAACCTCTTAGTAATAATATTTCGTTCTTTGGTGATGGTTTACCTTAACTAATGCTTACCTGTTTCTTTTTCTTTAATCCCAATCCGGTTTGGCCTCGCTTGCTCCAAAAAAAAAAAACCAAACGAAATGCAAACAG
Seq C2 exon
TTGCCCTCAAACGGCGCATTATCCTTGCAGGAAGCTGTCATAAACGAGCTGAAGAGCAAGGCGCACTCGGTGCGCGAGAAAAAGCTGCTGCAGCAGGACGAGGTGTACAACGGAGCCCTGGAGGACATCCTGCTCGGCCTGAAGAGCGAGCCGTACAGGCGGGCGGATGCTGTGCGGCGGTCGCAGCGCCGGAGGATCGACAATAATCGTTTATCGCGCACCCTGGAGGAAATGGATTGTTAGGACGGAAAACGAGATAGAGATCGACGCAATGACGACGACGGGGCAATGGCAACGAATCGAAAATCAAAATTCACACTGAAACCGAAACAAAAAGAGAACATTACAGATACTGCAATATGTGATTAGGCGATTAAATTGTTTTCGAGACACTCTTACACATACACAAATTCACATTCCCTTTTATGCAAATCCTTATATTTTCGAATATTTTTCAATATATTTTTAAGTAAAGTCC
VastDB Features
Vast-tools module Information
Secondary ID
FBgn0267795:FBtr0075775:8
Average complexity
IR
Mappability confidence:
NA
Protein Impact
Alternative protein isoforms
No structure available
Features
Disorder rate (Iupred):
C1=0.485 A=NA C2=0.331
Domain overlap (PFAM):
C1:
NO
A:
NA
C2:
NO
Main Inclusion Isoform:
NA
Main Skipping Isoform:
FBpp0075517
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
FBpp0075518, FBpp0113065
Associated events
Conservation
Human
(hg38)
No conservation detected
Human
(hg19)
No conservation detected
Mouse
(mm10)
No conservation detected
Mouse
(mm9)
No conservation detected
Rat
(rn6)
No conservation detected
Cow
(bosTau6)
No conservation detected
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GGCCGCTTCCAAGAAAGAGAA
R:
GTCGTCATTGCGTCGATCTCT
Band lengths:
346-1310
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Neural diversity
- Neurogenesis
- Neuronal activity
- Splicing factor regulation (brain)
- Splicing factor regulation (SL2)