DmeINT0011449 @ dm6
Intron Retention
Gene
FBgn0261570 | CG42684
Description
The gene raskol is referred to in FlyBase by the symbol Dmel
askol (CG42684, FBgn0261570). It is a protein_coding_gene from Dmel. It has 10 annotated transcripts and 10 polypeptides (9 unique). Gene sequence location is X:17710144..17752007. Its molecular function is unknown. It is involved in the biological process described with: negative regulation of Ras protein signal transduction; regulation of GTPase activity. 37 alleles are reported. The phenotypes of these alleles manifest in: border follicle cell; filamentous actin. The phenotypic classes of alleles include: cell adhesion defective; viable; behavior defective; smell perception defective; fertile; cell migration defective. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 06-12 hour embryonic stages.
Coordinates
chrX:17746571-17746898:+
Coord C1 exon
chrX:17746571-17746691
Coord A exon
chrX:17746692-17746770
Coord C2 exon
chrX:17746771-17746898
Length
79 bp
Sequences
Splice sites
5' ss Seq
GAAGTAAGT
5' ss Score
9.82
3' ss Seq
ATTGTTTTCCTTTGCCACAGATC
3' ss Score
10.39
Exon sequences
Seq C1 exon
ATACGTCCTATGAGAAGGCGTGCCGCCGTGGATCAGCGCCCACCACGCCCATTTTGGGCAGCAAACAGCACCAGACGGAGCACAATGCCACCTCGCGTTTCACCAACTTCTTTTCCAAGAA
Seq A exon
GTAAGTTCCCAGTATAATATATATATATATATTCAGGTTCACAATATTAATAATATGCCATTGTTTTCCTTTGCCACAG
Seq C2 exon
ATCCAATCCTTTGAAGCGGACCAAGTCGGTGACCAAGCTGGAGCGGACCAAGCGCGGATCCGGCGGACTGAGGGGCTCCCGCTCGCACGAGAGTCTGCTGTCCAGTCACGCCGTCATGTCCACCATAG
VastDB Features
Vast-tools module Information
Secondary ID
FBgn0261570:FBtr0302833:2
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.696 A=NA C2=0.583
Domain overlap (PFAM):
C1:
NO
A:
NA
C2:
NO
Main Inclusion Isoform:
NA
Main Skipping Isoform:
FBpp0309496
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
FBpp0291973, FBpp0291974, FBpp0291975, FBpp0291976, FBpp0291977, FBpp0309493, FBpp0309494, FBpp0309495
Associated events
Conservation
Human
(hg38)
No conservation detected
Human
(hg19)
No conservation detected
Mouse
(mm10)
No conservation detected
Mouse
(mm9)
No conservation detected
Rat
(rn6)
No conservation detected
Cow
(bosTau6)
No conservation detected
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CCATTTTGGGCAGCAAACAGC
R:
TGACTGGACAGCAGACTCTCG
Band lengths:
181-260
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Neural diversity
- Neurogenesis
- Neuronal activity
- Splicing factor regulation (brain)
- Splicing factor regulation (SL2)