DmeINT0018730 @ dm6
Intron Retention
Gene
FBgn0036910 | Cyp305a1
Description
The gene Cyp305a1 is referred to in FlyBase by the symbol DmelCyp305a1 (CG8733, FBgn0036910). It is a protein_coding_gene from Dmel. It has 2 annotated transcripts and 2 polypeptides (1 unique). Gene sequence location is 3L:19810403..19813339. Its molecular function is described by: iron ion binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; heme binding. It is involved in the biological process described with: oxidation-reduction process. 6 alleles are reported. The phenotype of these alleles manifest in: eye. The phenotypic classes of alleles include: viable; visible. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of very low expression. Peak expression observed in adults stages of both sexes.
Coordinates
chr3L:19812170-19813339:-
Coord C1 exon
chr3L:19813109-19813339
Coord A exon
chr3L:19812208-19813108
Coord C2 exon
chr3L:19812170-19812207
Length
901 bp
Sequences
Splice sites
5' ss Seq
CAGGTGAGT
5' ss Score
10.67
3' ss Seq
GAATTTCATTCCTAAAACAGGAC
3' ss Score
5.67
Exon sequences
Seq C1 exon
ATCAGTTGGCATAGAGCTGGCGTGGCATACGGACGCTCTGACACCTTGGCTTCCCAACCAGTGAACGTTTTATTGGACAAAAGCCTTGCTTGGACGTCGGTCTCGTTTTTCGGTGGTCTTATAAATAGTATATTGAAATGAGTGCCCTCATATTTCTGTGCGCGATTCTAATTGGTTTCGTGATTTATTCACTCATCAGCTCGGCGCGGCGTCCCAAGAACTTTCCACCAG
Seq A exon
GTGAGTTCTTCTCGTGCTGCACTTTGGAAAACTTTTCCTGGAATCCCAAATGCAGATAAGAAGTTAAAGCGTTGGTGCACAAACAAACGATGGATTTGCTTTAAAATTCGTAGAATACCAATTTTGTGTATCGTGGGTACAGTTTGCTTTTGAAAACTAGCCAATTAATTATATTTAATAGAAGAGAAGCATTATTCTTTGCATTAATAACCATATTATTATAAAATATATTTGTATATAGGCGAACATACGACCTGCCCCGCGACCAATTAATTGGTGGGCTCATTAGCAACTCGTAATGGTGATCAGAGGCATCTTTGGCCAATCGGCGGGGAACCAGACGGCACACGCTTTGAATGCTAATATATCTTATTGATAAAATCGTCACATACTTCTGACACCTGGCCTAACTGGTCTGAATTGGCCAGTAACAAGGGCCGGGCTGATTTATTCCACTTACCAATGCAGCTTTTGATTTGCATTAGGATTACGATTATGGGCGTAATAGCTGTCATTCCTGTATATAAGCACATTTTTATTATTATTCGCAATTGTTGCAGTTCAAAATGGTGATAAATTAGTATGCAATTTCTAAGGGAATCTTATTCACCTAAAGAAAAGCGGTCTCAATTTGACCACATATAAAAGTTATGAAATTGATTTTGATAACGCATTGATAGTTAATAACTAAGCGGTTTGATTTCAAGATAGAGTTTTCACTTTCGTTTCGGTAGTTTTGCTTGCTAACAGATTTCTTTTGCAGGCGATTAGATTAGCACTTTGATACCTATGCATAGGTTGAATTGATTGAATTGGACGGTTGTATTCCATATGAAATTCTATTGCTAACAAGACAAATATTTAAAATTAGTATCATTTTTGAATTTCATTCCTAAAACAG
Seq C2 exon
GACCTCGATTTGTGCCATGGTTAGGAAACACATTACAG
VastDB Features
Vast-tools module Information
Secondary ID
FBgn0036910:FBtr0074925:1
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion (1st CDS intron)
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0006717=p450=PU(0.4=6.2)
A:
NA
C2:
PF0006717=p450=FE(2.5=100)
Main Inclusion Isoform:
NA
Main Skipping Isoform:
FBpp0074694
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Human
(hg38)
No conservation detected
Human
(hg19)
No conservation detected
Mouse
(mm10)
No conservation detected
Mouse
(mm9)
No conservation detected
Rat
(rn6)
No conservation detected
Cow
(bosTau6)
No conservation detected
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ATCAGTTGGCATAGAGCTGGC
R:
TGTGTTTCCTAACCATGGCACA
Band lengths:
263-1164
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Neural diversity
- Neurogenesis
- Neuronal activity
- Splicing factor regulation (brain)
- Splicing factor regulation (SL2)