DmeINT0020414 @ dm6
Intron Retention
Gene
FBgn0040372 | G9a
Description
The gene G9a is referred to in FlyBase by the symbol DmelG9a (CG2995, FBgn0040372). It is a protein_coding_gene from Dmel. It has 3 annotated transcripts and 3 polypeptides (2 unique). Gene sequence location is X:245978..254650. Its molecular function is described by: zinc ion binding; histone-lysine N-methyltransferase activity. It is involved in the biological process described with 12 unique terms, many of which group under: behavior; developmental process; system process; tissue development; regulation of response to external stimulus. 56 alleles are reported. The phenotypes of these alleles manifest in: somatic cell; neuron; acellular anatomical structure; cuticular specialization; membrane-bounded organelle. The phenotypic classes of alleles include: increased mortality during development; phenotype; short lived; immune response defective. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of low expression. Peak expression observed within 00-06 hour embryonic stages, in adult female stages.
Coordinates
chrX:248382-250535:+
Coord C1 exon
chrX:248382-250238
Coord A exon
chrX:250239-250293
Coord C2 exon
chrX:250294-250535
Length
55 bp
Sequences
Splice sites
5' ss Seq
CAGGTAAAG
5' ss Score
9.65
3' ss Seq
ATACTTTAATTATATTTCAGGGA
3' ss Score
7.84
Exon sequences
Seq C1 exon
GTACAAGTTTCGCCCGGTGGAACGCGACGGTCTCGAAGAACACCCAGACCCATAGACACGCCCACTTCAGTTACTGATGAACATGTTCAGGTGGAGAATAAAAAATTTGGAAAGTCGGAGCAGTATACGGATTGTAGTAGCCATCTGGAGAGGTTCACACTGGACGATAATACTGCTATTGTCAGACTGCAGTTGAAAAGTGAACCGGACAAACCGTCATTAACTGCATTATCTCCTGAGGAAAACTCGGCTCCTGCTCCGAAGCGAGGAAGGGGTCGAGCCAGGAAAATAAGACCCGATGCCGAGGTGGAAACCAGTGAGGTTATTTTACCCTGCGAGGATTCATTAGGGGAGAAGAAACCAGGACGCAAAAGGAAACTCCCGGATGAACCGATAGATCAGCAGCAACTAAGCGACCTTGTCGTGGTAAAAACAGAGCAGGAGGAATTGGGTGACGCTCCCTTGGGAGATGTTAAGAGAATGCGACGTAGCGTTCGTTTAGGCAATCGATTACACGCCGATGGAAGCCCTTGGGAGGAGGTAAAGACAGAGGCACTTCACCCGCAGCCTTCTGCAGAACTTTCCTTTGCGGAGGTCACTTCTGAGATCTTGCCACTAGCTGTCTTAGATGAGAAGACCCCACCCAAAAAGCGAGGGCGGAAGGCAAAAACGCCCTGTGTGAAGTTAGAATCTGAAACATCCTGCGGGCTACCGTTTGCTAATGGCAACAAGAAAACGAATTCCTCTGGTGGATGCGAACTTCAGCTGCCCAAGCGTTCAAAAAGAAGAATAAAACCAACGCCAAAGATATTAGAAAATGACGAACTGCGCTGTGAGTTCGAAACGAAGCACATTGAGCGTATGACCCAGTGGGAGAGTGCTGCAGCAGTAGATGGGGATTTTGAAACGCCAACAACAGGCGGTAATGGGTCCAATTCCTCGACCTCAAGGCAAAAGAGCGACAAGTCCGACGGGAGCAACTTCGAAGGTGGTCCAGGACATCCTGCAGGCACAAGTGCCATAAAGAAAAGACTATTTTCAAAGTCCCAGCGGGACATTGAGAACTATGGCGCAGCAATGCTAGCCAAAAGTAAGTTACCTCCGTGTCCGGATGTCGAACAATTTCTAAACGACATCAAGGCATCTCGAATTAACGCGAATCGGTCACCAGAAGAAAGGAAATTGAACAAAAAACAGCAACGAAAACTGGCCAAACAAAAGGAAAAGCACCTGAAGCATTTGGGCCTGCAAAAAAATCACAGAGATGAGCCCTCGGATAACGACAGTTCCAATACGGACAATGAATTCTTTCCTACTACAAGGGTGCAAGTGGGAAAACCAAGTGTTACTTTGAGAGTAAGGAATAGTGTTACCAAGGAGCTTCCCACCACAGCCACTTTGAAATCACGCCGGAATCCAGTCGTGCAGGCAGCCAAACTCACGAGAAGGATAGGTGCAAGAGCCGCCGGTGAAGTTACTGAGGCAGCCCGAGCAAGTGTGCCAATTTCTACACCTGATGCGGAGCAGCTTCACTCGTTGGACACTTCTATTCAAGCTGATGTTACGCCCATAAGAGACTTAGATATGAGGCCCAGCACCTCCAGAGTGTCAAAGTTTATTTGTCTATGTCAGAAGCCTTCCCAGTATTATGCCAGGAATGCCCCGGATTCATCATACTGCTGTGCCATCGATCACATCGACGACCAAAAAATTGGCTGCTGCAACGAGCTGTCCTCAGAGGTTCACAATCTACTCAGACCCAGCCAACGCGTTAGCTATATGATCCTATGCGATGAGCACAAAAAGCGCCTACAATCACACAACTGCTGCGCAGGATGTGGCATATTTTGTACACAG
Seq A exon
GTAAAGACAGAATTATTATTGTGAACTGCAATCAAATACTTTAATTATATTTCAG
Seq C2 exon
GGAAAATTTGTGCTATGCAAGCAGCAGCACTTCTTTCATCCCGATTGTGCCCAGCGATTTATTCTAAGCACTTCGTACGAGAAGGAACTCGGCGATGAGGAAGATCAGGGCGTCAAATTCAGCAGCCCTGTCTTAGTGCTTAAGTGCCCCCACTGCGGATTAGATACACCAGAACGGACCTCCACGGTGACCATGAAGTGTCAGTCCCTGCCTGTCTTTCTGCGCACGCAAAAGTACAAGAT
VastDB Features
Vast-tools module Information
Secondary ID
FBgn0040372:FBtr0070063:2
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.777 A=NA C2=0.012
Domain overlap (PFAM):
C1:
NO
A:
NA
C2:
NO
Main Inclusion Isoform:
NA
Main Skipping Isoform:
FBpp0305207
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
FBpp0070062
Associated events
Conservation
Human
(hg38)
No conservation detected
Human
(hg19)
No conservation detected
Mouse
(mm10)
No conservation detected
Mouse
(mm9)
No conservation detected
Rat
(rn6)
No conservation detected
Cow
(bosTau6)
No conservation detected
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGCGCAGGATGTGGCATATTT
R:
TGATCTTCCTCATCGCCGAGT
Band lengths:
137-192
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Neural diversity
- Neurogenesis
- Neuronal activity
- Splicing factor regulation (brain)
- Splicing factor regulation (SL2)