DmeINT0021091 @ dm6
Intron Retention
Gene
FBgn0026430 | Grip84
Description
The gene Gamma-tubulin ring protein 84 is referred to in FlyBase by the symbol DmelGrip84 (CG3917, FBgn0026430). It is a protein_coding_gene from Dmel. It has 7 annotated transcripts and 7 polypeptides (5 unique). Gene sequence location is X:19559431..19565764. Its molecular function is described by: gamma-tubulin binding; microtubule minus-end binding. It is involved in the biological process described with 10 unique terms, many of which group under: cellular process; cellular component organization; cellular component assembly; microtubule cytoskeleton organization; reproductive process. 12 alleles are reported. The phenotype of these alleles manifest in: trichogen cell. The phenotypic classes of alleles include: increased mortality during development; phenotype; cell cycle defective; lethal. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of low expression. Peak expression observed within 00-06 hour embryonic stages, in adult female stages.
Coordinates
chrX:19564773-19565764:-
Coord C1 exon
chrX:19565414-19565764
Coord A exon
chrX:19565308-19565413
Coord C2 exon
chrX:19564773-19565307
Length
106 bp
Sequences
Splice sites
5' ss Seq
GCAGTAAGT
5' ss Score
9.07
3' ss Seq
TGTCATTTTTTATCCCACAGATC
3' ss Score
12.34
Exon sequences
Seq C1 exon
CAAATGCCAATTTGCATCAAGTTGCTTGTGCTGCTCATTTCAGTTTAAATTGATTCCGAAATTGCTGCAAGCGCGTAAAATAAAATGAGCATCTGCGATTTTAAAACGGCTAAGGATTGAAAAAAAAACTAGAACATTACAAGGACAGATGACAACCCTGTCGCTGCAGTCGGCACTCAAGCAACCCGGAAAAGAACAACAAAATATTGAGAAGAGCGGCGCACTCAAAAAGAGCTCAATTGCCGGTTATAACTGTAAATTGAGAAGCGCGGAACTATAAATTGCCATCTCCGTGATGAGTAAACCCCATGCGCTGCGCGGTGTGCTCGCCAATTTGATCAGCGAGTCGCA
Seq A exon
GTAAGTGGATTGAGTGCACAGGTGGCCAGTCGATCTAGCCGTTGCTCCTGCACCGCCTCTTTCCCGCCATTGGCACCATAGTTTACTGTCATTTTTTATCCCACAG
Seq C2 exon
ATCGCCCCTAACTCCGGAGAGCATCATTCGGGAGTTTCGGGCCACTAGTGATCGGCGGCTGCCAGCCTGCGATCTGATCAAAGTCCTGGTGTCCATCAGCGAGAAGCGTCCCAACTTCGCAGAGGTGACCAAGGAGATCTCGCACATTCTGCAGAATAAGGATCCCATGTACGCACTGCTGGTGTTCGTCGAGCGATACAGCGAATGCAAACCAGTCTCCGAGAGCAGCAGTTCCACTAGCCTTTCAGCGATGGGCTTGCCGCACGGAAACAAGACTTCGGATGTTAATTCCGCAGCCGGTTCAGTCCCCACCACATTGGCAATCGCAAGCACCTCGACAATCTTAACCACATCGCAAAACGTATCTGGTTCCACCAGGCTTAGCTTGACCCAGTCCCAGGACTTTCCCACCTCGACGCCGGTGAATTGCAAAAAGGCCACTGAATCCGACACTACACCCGTAGTCTTTGTCAGGCGCGGCCCAATGGACCGTAACCGTGGAGCGGGCAAGGATGAGCGCAACGACTTAAGTGTG
VastDB Features
Vast-tools module Information
Secondary ID
FBgn0026430:FBtr0074775:1
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion (1st CDS intron)
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.541
Domain overlap (PFAM):
C1:
NO
A:
NA
C2:
NO
Main Inclusion Isoform:
FBpp0074545
Main Skipping Isoform:
FBpp0074544
Other Inclusion Isoforms:
FBpp0074546, FBpp0310187
Other Skipping Isoforms:
FBpp0290522, FBpp0290523
Associated events
Conservation
Human
(hg38)
No conservation detected
Human
(hg19)
No conservation detected
Mouse
(mm10)
No conservation detected
Mouse
(mm9)
No conservation detected
Rat
(rn6)
No conservation detected
Cow
(bosTau6)
No conservation detected
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GTGCTCGCCAATTTGATCAGC
R:
TCGGAGACTGGTTTGCATTCG
Band lengths:
251-357
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Neural diversity
- Neurogenesis
- Neuronal activity
- Splicing factor regulation (brain)
- Splicing factor regulation (SL2)