DmeINT0024864 @ dm6
Intron Retention
Gene
FBgn0085370 | Pde11
Description
The gene Phosphodiesterase 11 is referred to in FlyBase by the symbol DmelPde11 (CG34341, FBgn0085370). It is a protein_coding_gene from Dmel. It has 7 annotated transcripts and 7 polypeptides (4 unique). Gene sequence location is 2L:18532025..18590342. Its molecular function is described by: 3',5'-cyclic-GMP phosphodiesterase activity; 3',5'-cyclic-AMP phosphodiesterase activity. It is involved in the biological process described with: cAMP metabolic process; signal transduction; cGMP metabolic process. 21 alleles are reported. The phenotypes of these alleles manifest in: mesothoracic tergum; chaeta. The phenotypic classes of alleles include: phenotype; increased mortality; some die during P-stage; increased mortality during development. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of low expression. Peak expression observed within 00-06 hour embryonic stages, during late larval stages, at stages throughout the pupal period, in adult female stages.
Coordinates
chr2L:18540629-18541620:-
Coord C1 exon
chr2L:18541185-18541620
Coord A exon
chr2L:18540769-18541184
Coord C2 exon
chr2L:18540629-18540768
Length
416 bp
Sequences
Splice sites
5' ss Seq
CTGGTGAGT
5' ss Score
10.1
3' ss Seq
GTGCTGTTTATCGCCACCAGGTG
3' ss Score
7.97
Exon sequences
Seq C1 exon
AAAAATATCTGCTCACGAGTTTGAGCGTGGCGGACTGCTGAAACCGATTGTCAACACAATCGACGGCACGCCCACATTTTTGAGCATTGGTCCGCCGATGGACAACGGCAGCGTAGGCGGCAGCTGCAGCAATCTGCAGAATGTCGGTGGCGTTGTGGCCGGACAGTATCAGTATAACCATCAGCAGCACCACCACAATCATGCCCATCTGCACCACAGCCAGCACAGTCATTACCAGGCGGGCGGGGCCGTTGGGAGCAGCAGCTTGGGCAGCACCGGTGGCGCTTCGGGAGCGGGAGGAGCACCCAGTCTGGGTGGCAGCGGTGGAGCCGGAAATGGCCATCAATATCCATACTATCATTGCCACCAGCGACCACAGCGGTTGTCACGCAACGAGCTCAAGCAGCTGGACGAGAAGGAGTTGATTTTCGAGCTG
Seq A exon
GTGAGTGAAAACAATAATATTTTTGATTATAAGAAAAATATGTTTAAGTTGTCCGATCACGTTCTGATCAAAATAATAATAGCCCAATATGATTCAAAAGTGCAATTGATTCGGAAATAACGTGACACACTCGCTTATATAAAAGTAATTGAATAAGTGAATGAGTAAAGTTTCCTCTAAATGCTTAACCATTAGATATCAAAGTGAAGGTTCCGGCGAATCTCGATAGCTCGTCAATGATTATTTGTTGAGTTCCGCAAACGTGTTTGTTTGTCGGCCCATTGACGCTGTTATCGGCAAACACTCGAAAGACGTCAGTCACACAGTCTGTATTTCCCCATGGCTCCCTTTCTTTTTCAAATGCAACTGCTTATCAGTCGTGTAAATAACCGGACTGTGCTGTTTATCGCCACCAG
Seq C2 exon
GTGAAGGACATTTGCAACGAGCTCGAGGTGCGCACTTTGTGCCACAAGATACTCCAGAACGTGTCCATTCTGCTGAATGCCGATCGAGGATCTCTGTTCCTGGTGCAAGGACGATGCAACGGACCGGATGGCCTAAAAAA
VastDB Features
Vast-tools module Information
Secondary ID
FBgn0085370:FBtr0112544:5
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.630 A=NA C2=0.000
Domain overlap (PFAM):
C1:
NO
A:
NA
C2:
PF0159021=GAF=PU(24.7=80.9)
Main Inclusion Isoform:
NA
Main Skipping Isoform:
FBpp0111456
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
FBpp0111457, FBpp0292261, FBpp0292262, FBpp0310380, FBpp0310381
Associated events
Conservation
Human
(hg38)
No conservation detected
Human
(hg19)
No conservation detected
Mouse
(mm10)
No conservation detected
Mouse
(mm9)
No conservation detected
Rat
(rn6)
No conservation detected
Cow
(bosTau6)
No conservation detected
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ACCACCACAATCATGCCCATC
R:
AACAGAGATCCTCGATCGGCA
Band lengths:
346-762
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Neural diversity
- Neurogenesis
- Neuronal activity
- Splicing factor regulation (brain)
- Splicing factor regulation (SL2)