DmeINT0025075 @ dm6
Intron Retention
Gene
FBgn0015278 | Pi3K68D
Description
The gene Phosphatidylinositol 3-kinase 68D is referred to in FlyBase by the symbol DmelPi3K68D (CG11621, FBgn0015278). It is a protein_coding_gene from Dmel. It has 6 annotated transcripts and 6 polypeptides (2 unique). Gene sequence location is 3L:11774650..11790013. Its molecular function is described by 9 unique terms, many of which group under: transferase activity, transferring phosphorus-containing groups; transferase activity; binding; protein binding; phosphatidylinositol kinase activity. It is involved in the biological process described with 7 unique terms, many of which group under: cellular process; organophosphate metabolic process; cell communication; primary metabolic process; biosynthetic process. 25 alleles are reported. The phenotypes of these alleles manifest in: endomembrane system; intracellular vesicle; sensillum campaniformium; bristle of mesothoracic tergum; neuromuscular junction. The phenotypic classes of alleles include: increased mortality during development; phenotype; increased mortality; visible. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of moderate expression. Peak expression observed within 00-06 hour embryonic stages, in adult female stages.
Coordinates
chr3L:11781391-11782129:-
Coord C1 exon
chr3L:11781884-11782129
Coord A exon
chr3L:11781828-11781883
Coord C2 exon
chr3L:11781391-11781827
Length
56 bp
Sequences
Splice sites
5' ss Seq
AAGGTAAAT
5' ss Score
8.88
3' ss Seq
ATAACTATAAACTTTCTTAGTGC
3' ss Score
3.44
Exon sequences
Seq C1 exon
AAACTTACTACTATTGCGGAGTCGGTTAAAGAGGCTAAGGAGTCGATGCGTCAAAAAAGCCTAGCCGCAGGCATGGACGAGGTGCACCAAGACTTACTGGAGCAACCCACTTGCCTACCGCTGGGACCAGAACTGGAGGTAACTGGAGTGAGTGTGCGTAACTGTAGCTACTTTAACTCCAACACGCTGCCGCTGAAGATCAACTTTGTGGGACCTGATGCCGAATCTTTACCGGCTATCTTTAAG
Seq A exon
GTAAATATTCATTCACAAATGATATGTATAAACTATATAACTATAAACTTTCTTAG
Seq C2 exon
TGCGGAGATGACTTGCAGCAGGATCAGTTAACTATACAGCTAATTAGGATTATGAACAAAATGTGGTTGGCCGAACGATTGGACCTGAAGATGGTCACCTTTAATTGTGTGCCTACGGGATACAAGAGCGGTATGATTGAGCTGGTTAGCGAGGCGGAAACGTTGAGAAAAATTCAAGTAGAGTGCGGTCTGACGGGGTCCTTTAAGGATCGCCCGATCGCTGAGTGGTTAGGCAAGCAGAATCCCAGTCCTCTCGAGTACCAGAGTGCTGTGCGAAATTTTACGCTATCCTGTGCTGGATACAGTGTGGCCACGTATGTGCTAGGCATCTGTGATCGCCACAATGACAACATCATGTTAAAGACTTCGGGTCACTTGTTTCACATTGACTTTGGCAAGTTTCTTGGCGATGCTCAGATGTTTGGAAACTTTAAGAG
VastDB Features
Vast-tools module Information
Secondary ID
FBgn0015278:FBtr0076088:9
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.037 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0045422=PI3_PI4_kinase=PU(2.8=7.3)
A:
NA
C2:
PF0045422=PI3_PI4_kinase=FE(67.1=100)
Main Inclusion Isoform:
NA
Main Skipping Isoform:
FBpp0075818

Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
FBpp0075819, FBpp0291030
Associated events
Conservation
Human
(hg38)
No conservation detected
Human
(hg19)
No conservation detected
Mouse
(mm10)
No conservation detected
Mouse
(mm9)
No conservation detected
Rat
(rn6)
No conservation detected
Cow
(bosTau6)
No conservation detected
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AACTTTGTGGGACCTGATGCC
R:
AGGTGACCATCTTCAGGTCCA
Band lengths:
145-201
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Neural diversity
- Neurogenesis
- Neuronal activity
- Splicing factor regulation (brain)
- Splicing factor regulation (SL2)