DmeINT0025728 @ dm6
Intron Retention
Gene
FBgn0015791 | Rab14
Description
The gene Rab14 is referred to in FlyBase by the symbol DmelRab14 (CG4212, FBgn0015791). It is a protein_coding_gene from Dmel. It has 4 annotated transcripts and 4 polypeptides (2 unique). Gene sequence location is 2L:14355145..14358764. Its molecular function is described by: GTP binding; GTPase activity. It is involved in the biological process described with 9 unique terms, many of which group under: intracellular transport; response to external stimulus; inorganic ion homeostasis; cell communication; peptide transport. 15 alleles are reported. The phenotypes of these alleles manifest in: phagocytic vesicle; hemocyte. The phenotypic classes of alleles include: fertile; wild-type; flight defective; immune response defective; viable. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed in adult male stages.
Coordinates
chr2L:14357341-14357700:+
Coord C1 exon
chr2L:14357341-14357454
Coord A exon
chr2L:14357455-14357519
Coord C2 exon
chr2L:14357520-14357700
Length
65 bp
Sequences
Splice sites
5' ss Seq
AATGTGAGT
5' ss Score
7.8
3' ss Seq
CTCTACTTGCCGTCCTGCAGTCA
3' ss Score
6.86
Exon sequences
Seq C1 exon
GTCCCAACATGACTGCAGCGCCATACAACTACAACTATATCTTTAAATACATCATCATTGGTGACATGGGCGTGGGCAAGTCCTGCCTGCTCCACCAGTTCACCGAGAAGAAAT
Seq A exon
GTGAGTAGACTGTGCTGAGTAATAATGTATGTAATAAGAACTAATCTCTACTTGCCGTCCTGCAG
Seq C2 exon
TCATGGCCAATTGTCCTCACACCATTGGCGTGGAGTTCGGCACACGCATCATTGAGGTGGACGACAAAAAGATCAAGCTACAGATCTGGGACACAGCGGGTCAGGAGCGATTCAGGGCAGTGACACGCTCCTATTACCGTGGAGCAGCTGGTGCGCTGATGGTCTACGATATTACCAGGCG
VastDB Features
Vast-tools module Information
Secondary ID
FBgn0015791:FBtr0080626:2
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion (1st CDS intron)
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0007117=Ras=PU(14.2=63.9)
A:
NA
C2:
PF0007117=Ras=FE(37.0=100)
Main Inclusion Isoform:
NA
Main Skipping Isoform:
FBpp0080200
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
FBpp0080199, FBpp0310268
Associated events
Conservation
Human
(hg38)
No conservation detected
Human
(hg19)
No conservation detected
Mouse
(mm10)
No conservation detected
Mouse
(mm9)
No conservation detected
Rat
(rn6)
No conservation detected
Cow
(bosTau6)
No conservation detected
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CTGCAGCGCCATACAACTACA
R:
TGTCGTCCACCTCAATGATGC
Band lengths:
168-233
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Neural diversity
- Neurogenesis
- Neuronal activity
- Splicing factor regulation (brain)
- Splicing factor regulation (SL2)