DmeINT0028261 @ dm6
Intron Retention
Gene
FBgn0052463 | Tengl2
Description
The gene Testis EndoG-Like 2 is referred to in FlyBase by the symbol DmelTengl2 (CG32463, FBgn0052463). It is a protein_coding_gene from Dmel. It has 2 annotated transcripts and 2 polypeptides (1 unique). Gene sequence location is 2L:2238304..2239384. Its molecular function is described by 6 unique terms, many of which group under: catalytic activity; endonuclease activity; nuclease activity; hydrolase activity, acting on ester bonds; binding. It is involved in the biological process described with: DNA catabolic process, endonucleolytic; apoptotic DNA fragmentation. One allele is reported. The phenotype of this allele manifest in: chaeta. The phenotypic classes of allele include: visible; viable. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderate expression to a trough of no expression detected. Peak expression observed during late pupal stages, in adult male stages.
Coordinates
chr2L:2238304-2239384:-
Coord C1 exon
chr2L:2239192-2239384
Coord A exon
chr2L:2239129-2239191
Coord C2 exon
chr2L:2238304-2239128
Length
63 bp
Sequences
Splice sites
5' ss Seq
GTGGTAACC
5' ss Score
3.88
3' ss Seq
AAAATAATTTCCCTAACCAGTTT
3' ss Score
5.62
Exon sequences
Seq C1 exon
AATTCACAGAGCCCATTTCATGGACCGAAAATGGCAGCTCCTGGGACTGGGTTTCGTCCTGGGAGTGACTGCGCTCTGTGCATCCTTCGTGGGTGGAATGTATTTTCAGCACACAGATGCCAGAAAACGCCTGCGGGAACTAATACAAACGGATCCCTATGCCTACTATCTCAGTCCCAAAATTTATGAAGTG
Seq A exon
GTAACCAATACTGTAGTAAAAGAATATAAGTAACACTTGCACCAAAATAATTTCCCTAACCAG
Seq C2 exon
TTTACTTTCTTTGAGAGCAATAATGAAGACGATCACCGGATGAGGCAGATCATGAAGTTTGGGTTCCCTGGCCTGGATGACTTGCGTCTGTATAGTGACTTCGTACTATCCTACGATCGCCGAAACCGAGTGGCCCACTGGGTGTGCGAGCACCTCCAGGCGGACAGCATACATCCGAATCGAGGCAGACGTGGTCGCAACCCATACCAGCCGGATTTGAGTGTTCCCTCCAACTTCCGATCGGAACTGAGCGACTATCGGAGATCTGGATTCGACCGAGGACACTTGGCAGCCGCAGGAAATCACCATCTGCAACAGAATCACTGCGAGGATACGTTTTTCCTAACGAATATTGCCCCACAAGTGGGCCAGGGCTTCAACCGAAGCGCGTGGAACAACTTGGAACAATATGTTCGAAATCTGGTCCACCGATTTGGATCCGTTTTCGTGTGTACTGGTCCACTATATAAGCCCAATCAAAGACCGGGAGGTAAGTGGGCCGTGGAGTACGAGATGATCGGACTGAATATGGTTGCAGTTCCGACGCACTTCTTCAAGGTGATCATGGTGGAATCAAAACTGCACCTGGGTAAACCCTATATGGAGGGCTATGTCCTGCCCAATGCTCCAATACCAGATGGTCTGCCACTACGATCGTTTCTCTGCGACATCCGGGAGATTGAGCACTACGCAGGTCTCAAGTTCTTCGATGGTTTGAGAAGGAGTGCCCTATTCGGCAGCAATTATCCAAGTGAATCTCGAGTTTTTCGCGAATTCAGTTAGTGGAGGGATCCCAATCGTTAAATATATATTTAACTTAACTGC
VastDB Features
Vast-tools module Information
Secondary ID
FBgn0052463:FBtr0077808:1
Average complexity
IR
Mappability confidence:
NA
Protein Impact
Alternative protein isoforms
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.062
Domain overlap (PFAM):
C1:
NO
A:
NA
C2:
PF0122318=Endonuclease_NS=WD(100=81.6)
Main Inclusion Isoform:
NA
Main Skipping Isoform:
FBpp0077485
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Human
(hg38)
No conservation detected
Human
(hg19)
No conservation detected
Mouse
(mm10)
No conservation detected
Mouse
(mm9)
No conservation detected
Rat
(rn6)
No conservation detected
Cow
(bosTau6)
No conservation detected
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CACACAGATGCCAGAAAACGC
R:
TGATCTGCCTCATCCGGTGAT
Band lengths:
136-199
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Neural diversity
- Neurogenesis
- Neuronal activity
- Splicing factor regulation (brain)
- Splicing factor regulation (SL2)