DmeINT0028264 @ dm6
Intron Retention
Gene
FBgn0037857 | Tengl4
Description
The gene Testis EndoG-Like 4 is referred to in FlyBase by the symbol DmelTengl4 (CG4683, FBgn0037857). It is a protein_coding_gene from Dmel. It has 2 annotated transcripts and 2 polypeptides (1 unique). Gene sequence location is 3R:10950284..10951797. Its molecular function is described by 6 unique terms, many of which group under: catalytic activity; nuclease activity; hydrolase activity, acting on ester bonds; endonuclease activity; hydrolase activity. It is involved in the biological process described with: apoptotic DNA fragmentation; DNA catabolic process, endonucleolytic. 5 alleles are reported. No phenotypic data is available. The phenotypic classes of alleles include: fertile; flight defective; viable. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of no expression detected. Peak expression observed during late pupal stages, in adult male stages.
Coordinates
chr3R:10950643-10951797:+
Coord C1 exon
chr3R:10950643-10950819
Coord A exon
chr3R:10950820-10950873
Coord C2 exon
chr3R:10950874-10951797
Length
54 bp
Sequences
Splice sites
5' ss Seq
GCAGTGAGT
5' ss Score
7.39
3' ss Seq
GTGACTTTGGATCATTTAAGATA
3' ss Score
4.12
Exon sequences
Seq C1 exon
TTGTCAATGTTTGGGCATTCGTCTAGGAAGTCAATTACAAGGACAGAATCTAATCAGACAGCAAGTATTGGAGACGAGTCTGGGACTAGCAAACGATATTCAGAAAGACAAGATCCTGGTAGTGTATCCATATTCGAACACTTTTTACTGTGGGGTCGCAGGAAGGCGCATAACGCA
Seq A exon
GTGAGTCGAAAATTAAATGCTGCAAGAATTGTTTGTGACTTTGGATCATTTAAG
Seq C2 exon
ATATCGAAGATGACATTATCTGTCGGGCCAAATGGAAGCAGACATGAGAATATTGAACATGTAGCTGATATTATGAAGTATGGATTTCCGGGCATGGATGAAATACACGTCTATAAAAACTTCGTACTCTCATACGATCGTCGCAATCGTATTGCCCATTGGGTATGTGAACATGTCAAAAGCGGATGCGTACAGGAGAGGGATGAAAAGACATTGAATAAGCCAAATGCCTACATAACAGACAATACAATACCAACGATATTTAGTGCAAATATGCGGGACTTTAAGAATTCCGATTGGGTTGGTGGTCACTTGGCCTCCCCTCAAAACTACAAATGCGATGCGCTGAAGTTTTTGGAGGCCTACAAGTTTACGAATATCGTGCCCATTAATCGAGGGCTGAAAAACCATATTTGGTACCGTTTGGAAAGCTATGTTAGAGACAAGGCGATCGAATTCGATTCAGTTCATGTTTATACCGGTCCTCTGTTTATGCCCCAAAGGATAACGTTCAGGAACTGGTCTGTGCGTTACCATGTAATGGGCATGAACACGGTTGCTGTTCCAACGCACTTCTTCAAAATCATCATAAGAGAGGATGAGTTTAATAGGGATATGCCCATTATGGAAGGTTATGTTGTTCCCAATGCATATGTCGACAAGGATATGGATCTGCGCTCATTTTTAGCCGATGTTCGTGATATTGAACACTTCGCAGGACTCAAATTCTGTGATGGCCAGCAGCGGGATCAACTTGAATTGCCTGTAAAACCGCCCTCAATGACGGATTTCCAGAACTGACTAAAGTGGTCACGTATTACAACGGTTTTTATAAATAAATAACACCTTTTGCCAAATTTTTTTTACCCACTGACCTATAAAAAGCTTTCCTCTATAAAAATGTAATAAATCTTGGCCGTTTTT
VastDB Features
Vast-tools module Information
Secondary ID
FBgn0037857:FBtr0082326:2
Average complexity
IR
Mappability confidence:
NA
Protein Impact
Alternative protein isoforms
No structure available
Features
Disorder rate (Iupred):
C1=0.356 A=NA C2=0.071
Domain overlap (PFAM):
C1:
NO
A:
NA
C2:
PF0122318=Endonuclease_NS=WD(100=80.1)
Main Inclusion Isoform:
NA
Main Skipping Isoform:
FBpp0081802
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Human
(hg38)
No conservation detected
Human
(hg19)
No conservation detected
Mouse
(mm10)
No conservation detected
Mouse
(mm9)
No conservation detected
Rat
(rn6)
No conservation detected
Cow
(bosTau6)
No conservation detected
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CGAGTCTGGGACTAGCAAACG
R:
ATGTCTGCTTCCATTTGGCCC
Band lengths:
148-202
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Neural diversity
- Neurogenesis
- Neuronal activity
- Splicing factor regulation (brain)
- Splicing factor regulation (SL2)