DmeINT0029140 @ dm6
Intron Retention
Gene
FBgn0037402 | Vha14-2
Description
The gene Vacuolar H[+] ATPase 14kD subunit 2 is referred to in FlyBase by the symbol DmelVha14-2 (CG1076, FBgn0037402). It is a protein_coding_gene from Dmel. It has 4 annotated transcripts and 4 polypeptides (2 unique). Gene sequence location is 3R:6122592..6124405. Its molecular function is described by: proton-transporting ATPase activity, rotational mechanism; ATPase-coupled ion transmembrane transporter activity. It is involved in the biological process described with: ion transmembrane transport; proton transmembrane transport. 4 alleles are reported. No phenotypic data is available. The phenotypic class of alleles includes: viable. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderate expression to a trough of no expression detected. Peak expression observed at stages throughout the pupal period, in adult male stages.
Coordinates
chr3R:6122934-6123422:+
Coord C1 exon
chr3R:6122934-6123179
Coord A exon
chr3R:6123180-6123237
Coord C2 exon
chr3R:6123238-6123422
Length
58 bp
Sequences
Splice sites
5' ss Seq
CAAGTGAGT
5' ss Score
9.1
3' ss Seq
CCTTACCAATTCTTCTCCAGCGA
3' ss Score
7.18
Exon sequences
Seq C1 exon
TTATCGCATTATTCTCTTATCGTTTTTCATTGTATACCAGACACCACCCCTAAGCAGATCGAAGAGTGTTTCAAAAAGTTCCTGAGGCGACCGGATATAGTCATTATTCTGATCAACCAGGTTTACGCTGATATGATTCGACCCACTGTCGATGCTCACAATCTGGCCGTTCCCACTGTGCTGGAGATTCCCTCGAAACAGCATCCCTACGATTCCTCCAGGGACTCGATTCTGAAGCGGGCTCAA
Seq A exon
GTGAGTTATCCTGCACCGTCAAAGGGTTAGCCCCAAGTCCTTACCAATTCTTCTCCAG
Seq C2 exon
CGAGTCATCACTCCGCCCGAGAGGCACTACTAGCGGCTCAGTTTTGGCAGTCACTTTTGGGGAGGTGGGGAAGTTTAATGAAGGATTTTGCATAGTTTTCAAATGCATACCAAATGCTGGTGATACAATGTCGTTGGAACAGTTATCGAAAATATATACGCACAAACATAGATATATTCACAAAG
VastDB Features
Vast-tools module Information
Secondary ID
FBgn0037402:FBtr0078585:2
Average complexity
IR
Mappability confidence:
NA
Protein Impact
Alternative protein isoforms
No structure available
Features
Disorder rate (Iupred):
C1=0.021 A=NA C2=0.171
Domain overlap (PFAM):
C1:
PF0199012=ATP-synt_F=FE(81.8=100)
A:
NA
C2:
PF0199012=ATP-synt_F=PD(0.1=0.0)
Main Inclusion Isoform:
NA
Main Skipping Isoform:
FBpp0078234
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
FBpp0301284, FBpp0301285
Associated events
Conservation
Human
(hg38)
No conservation detected
Human
(hg19)
No conservation detected
Mouse
(mm10)
No conservation detected
Mouse
(mm9)
No conservation detected
Rat
(rn6)
No conservation detected
Cow
(bosTau6)
No conservation detected
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGTCGATGCTCACAATCTGGC
R:
GCCAAAACTGAGCCGCTAGTA
Band lengths:
148-206
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Neural diversity
- Neurogenesis
- Neuronal activity
- Splicing factor regulation (brain)
- Splicing factor regulation (SL2)