DmeINT0030493 @ dm6
Intron Retention
Gene
FBgn0086757 | cbs
Description
The gene centrosomin's beautiful sister is referred to in FlyBase by the symbol Dmelcbs (CG4840, FBgn0086757). It is a protein_coding_gene from Dmel. It has 4 annotated transcripts and 4 polypeptides (3 unique). Gene sequence location is 2R:13450586..13453393. Its molecular function is described by: ADP-ribosylation factor binding; Rab GTPase binding. It is involved in the biological process described with: centrosome cycle; Golgi vesicle transport. 8 alleles are reported. The phenotypes of these alleles manifest in: spindle; centrosome; embryo; nucleus. The phenotypic classes of alleles include: viable; mitotic cell cycle defective; lethal - all die before end of embryonic stage; some die during embryonic stage. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of very low expression. Peak expression observed within 00-06 hour embryonic stages, in adult female stages.
Coordinates
chr2R:13451928-13452735:+
Coord C1 exon
chr2R:13451928-13452026
Coord A exon
chr2R:13452027-13452321
Coord C2 exon
chr2R:13452322-13452735
Length
295 bp
Sequences
Splice sites
5' ss Seq
TCTGTGAGT
5' ss Score
7
3' ss Seq
AATAAACCGAATGTTCTCAGCAA
3' ss Score
0.75
Exon sequences
Seq C1 exon
GTCAAGCTGCTGGAGGAGGAAATCGATGAACTTAGTGTGGCCCTGAAAGAGTGCCGAGAGGAGAACGAGCAGCAAGTCCTCTTTGAGCGCAATAAATCT
Seq A exon
GTGAGTATGCCTCCCGATTCTGACGATTTCCATTGGGACTTGTAACACTAATTTAACCAACAATGCAGTACTAACTCTACCAATTAAGCTCCTGGTTTACTTGGAATTTTCCAACCACACCTATTATGTAGTTTTTGATTGTAAATGTTTTCCTTTTCGTATTAAACTGATTTAATCGTTGGCAAACTTTTTGGTAAACTTTTTCCTTCAATAATGCACGCATATATATGTTTACCCTCAATATCCAAAACTGATTGATGGAGATTTTGTAAACCAATAAACCGAATGTTCTCAG
Seq C2 exon
CAAAATTTGGAAACAGAGGTAAAAGATCTCAAGACTCGTCTCACGGCAGCGGATGACAGGTTCAGCGAGTACTCCTCGAATGCCGAGCAGGTGGCCCAAAAGCTACGGGTTCAGGTGACTGAAAAGCAGGAGCAGCTGGACGAAACCATAATGCAACTGGAAATCGAACGAGAGGAGAAAATGACTGCAATACTTCGCAATGCGGAAATCGCCCAGAGCGAAGACATTTTACGGCAACAGCTGCGCTTGGAGCGCAGCGAAGCCAGCGACCTCCAGGAGAGGAATAATCAGCTGGTGAGGGATATATCCGAGGCACGGCAAACGTTGCAGCAGGTCAGCAGCACAGCACAGGATAATGCCGACAAGTTGACCGAATTCGAGCGAGTTCAGTTGGAAATAATCGAAAAGAATAAG
VastDB Features
Vast-tools module Information
Secondary ID
FBgn0086757:FBtr0087666:3
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.273 A=NA C2=0.384
Domain overlap (PFAM):
C1:
NO
A:
NA
C2:
NO
Main Inclusion Isoform:
NA
Main Skipping Isoform:
FBpp0086786
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
FBpp0301620, FBpp0301621
Associated events
Conservation
Human
(hg38)
No conservation detected
Human
(hg19)
No conservation detected
Mouse
(mm10)
No conservation detected
Mouse
(mm9)
No conservation detected
Rat
(rn6)
No conservation detected
Cow
(bosTau6)
No conservation detected
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TCAAGCTGCTGGAGGAGGAAA
R:
GCATTGCGAAGTATTGCAGTCA
Band lengths:
301-596
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Neural diversity
- Neurogenesis
- Neuronal activity
- Splicing factor regulation (brain)
- Splicing factor regulation (SL2)