DmeINT0034018 @ dm6
Intron Retention
Gene
FBgn0263490 | mld
Description
The gene molting defective is referred to in FlyBase by the symbol Dmelmld (CG34100, FBgn0263490). It is a protein_coding_gene from Dmel. It has 6 annotated transcripts and 6 polypeptides (4 unique). Gene sequence location is 3R:24585653..24623899. Its molecular function is described by: nucleic acid binding; zinc ion binding. It is involved in the biological process described with: ecdysone biosynthetic process; positive regulation of circadian sleep/wake cycle, sleep; long-term memory; determination of adult lifespan. 42 alleles are reported. The phenotypes of these alleles manifest in: mesothoracic tergum; prothoracic gland; trichogen cell; ring gland. The phenotypic classes of alleles include: behavior defective; visible; increased mortality; phenotype.
Coordinates
chr3R:24620379-24623899:+
Coord C1 exon
chr3R:24620379-24621113
Coord A exon
chr3R:24621114-24622921
Coord C2 exon
chr3R:24622922-24623899
Length
1808 bp
Sequences
Splice sites
5' ss Seq
GAGGTAAGT
5' ss Score
11.08
3' ss Seq
ATTTTTTAAATTTACATTAGCTC
3' ss Score
3.85
Exon sequences
Seq C1 exon
GCACTTCCGGCCACAGCCATGCCAACGAACAGTTCGAGGAGCTGCAGACACTGCAGGCGCCGCCAACGGTGTTGACTCCGCCATCGACCATAGTCTCGGTACCATCGCCACAGCCCATGGTGTACTCGCAGCACATAACAATGCCGTCGCCGGAACAATCCGAACCAGATTCCACAACCACGTTGCGACAGTATCGCAAGCGAGGCGTGATCGTTGGGCCACAGGGTCCGCTGCATTTGGCCACACCGGTGGCCTCGCCATCGCCCTCGTCGTCACCCTCCTCATCGACGGTGGATCACATACCGCCGGCATCTCCGGCTACGCCCGCATCACCAGCTCCGCCTCCGTCGCCCGCCGTCGCGTCCACGGTGCAAGTGAGCGAGCTGCGAACGAGTCACCATTGTCTGTACTGCGAGGAGCGATTCACCAACGAAATTTCCCTGAAGAAGCACCATCAGCTGGCGCACGGAGCGCTGACAACAATGCCATATGTGTGCACAATCTGCAAGCGAGGGTATCGCATGCGTACGGCGCTGCATCGGCACATGGAGTCGCACGATGTGGAGGGACGGCCGTACGAGTGCAACATTTGTCGCGTCCGGTTCCCGCGACCATCGCAACTGACGCTGCACAAGATCACGGTGCACCTGCTCTCCAAGCCGCACACCTGCGACGAGTGTGGCAAACAGTTTGGCACGGAGAGCGCCCTCAAGACGCACATTAAGTTCCACGGAG
Seq A exon
GTAAGTTTCGAGCACCACCAAAAGGGTTTAGTTCGTTGATTTTCTTCTTTTTTTTTTTTTGTCGGTTGCCTAGCAGTTAACCCACCATGTACTAATCCCCATTCATTTAGTCTTAGTGCTGCTCTGTAGTAGCTTGAGTTATAAGCCTCTTACTAATCCTCCATCAATTGTTATCGTTGTATTGTTGATGTCGCTGTTGTTATTGTTGGCGGCAGTTTATTCAAGCGTGGTAATATGTATACGTTCCAAAGGTATGACAACCGTGAACGAATCGCTTTCGTCTCGAGAATACCAATATCGATTTATGGCATGGGCATATTCCTAATGCTTGGCATGAAAATCACACAGCTTATGTCTTATGCTTTTAGCCTTAAGCCAAAATAAACCGTTTATGAACTTGCGCATGCAGTTTTGTGAGTGGATACAAAAAAAAAATGTAACAATAATAGTAGACAAGCCAGTTGGTGGTATGAATGCGCCCGGTCGGTCGAACGGCTTGCAAAAATTGTATAAACGCTAATTACCCGTTGATATGAACTCTGACAATTAAACGCTGATATCTGACGGCTCTAGCTCAGTAGCTCTAGCTCAGCTTGCCTAATTATTGACAATTAGCTGCCCTTTGTTATTAATCCGATGACCTGGACACTGGATCACTGATCACTGAACACACTCAACACACTTATTTTGTATTCAATTGGTAGATGTGGAGTGCGTTTCGTAACACGTGGGAATTTCTAAGAAAATGGTTGTTGAATATTCCATTGAATATACATTTATTAGCTGTACATATTACTTATACCTATGTATATATATAGATTGCTTGACTTCTGCTTTAACCCTTTCTACTTCAATGGAATTTCCTCTGGCTGAAACTCTGGATTTCGCCACGCACTTTCAACCCAAAATAAATAAAACCTCTAACAATCAATGTTACATGTTGGTTGTTCTTTGCCGGTACAGTGAAATCTTTGGTTTACAATTAAATAAAAACTAAAATTCGCTTGACACGAGTGTATTTATCGCTATCTATGTTGGCTCCCTTCAAGATCACACTGTGTGAATCCTAATGGCTCAACACAACAACCGAACTGAATATGTAGATACCGATACGATACGATAAAGATAAAGATACAAAGGCCAACGAAATTGTCTTTTCCAGAGCTCGGTTACCAGTGCGACGGGTGCGATCGGACGTTCGAGTATCTGAAAGAATTGCGCAAACATCGACGCACCCACAGCGAGATGTTTTACAAATGCAAATTCTGTCCAAGCTCCTTTATGCGTTTCACGAATTTTCGGGGTAAAGTCTCGCCGTTCATAAATGGTGTTGCCAACATTGTTAATTCGAATTTAAAAATACATTCATTACCAGAAGAGATTTGTTTTTCTTCTTTATCGAATTTTTATATTTTTATCTTAGCAATTATAATGAATTCCTTATATCATTTAAAGGTGTCAATGTAGCTTTAGTTTAAGAACTATTGATAGTTTTCAAAAAGCCTCAACCAAGTTATTATTTCAAATTCTATAATTGACTACTACTAGCTGTGTGAATGTTGTTTGTAATGTCCATTGTTCTTATCTACTTATGCAACTTAGATGTATCTACATAGTTGGCAGCACTATTCCAAATGTATTTAAGTAATAGGTTCTGCTCTAGTTTGTCGTGAGAAACGAGAGTAAGGATAGGTAAATTACAAATGCCGGCTTCTTGCATGGCCTACGTTAAGATGCAACAACTTAATTGAAGTTTTTGAAGCTAGTAAAACGTGTAATAACTAACTTATTTTTTAAATTTACATTAG
Seq C2 exon
CTCACATGAAAACCCATTTGCCGCTGGGCGTATTCCGCAACGAGGATGCCGCATCGAAGTCACCCAGCAACAACAATAGCCACGCCAGCAGTGACAAGCTGGAGAATCCAGCTACGCCCATCGAGGAGACACCTTTGACGCCGATGAGCAGTGGCGGCCACTTGTACCACAGCCCAGATGAATACCCCAATTCGGTGGAGAGCTGCGCTGGCAACAGTGTGGCTCTGGAATCCTATGCCACAACGCCATAACCCACGGGGCCGGATTATTATTTAGTGTACAACGCTATAGTTATAAGTAATCACAAAAAACCTAGCCAAATAATATCGGCGCCTTCCTACACTATTACCACACTCCGTGGGGAATAGCTTCCATTTATCCCATTGGAGAGCCAATCATTAAGTAACTCTTAACTCTTATTCTAAACAATATGAAAAGAAAACAAAAGAACCAGCAGACTGCATTGAATGCAATCCCATATTGCACTAAAAACAAAAGAACACCTAGCGACTAAGCCAAGGATACACTCCCTCAGACACAAATCTTAAGGCTACAAAATATTAGACCTAAGCTAGTTAGAATCTAGACATTCATGTAATGAAACCAACTGAAAGAAATACGCAAAGGTTAACTTTAGGCCTTAGACATATATATACATATACATACTTATTGCTGATTTCCGTTCCCAGTTAGATATATATATATATTTTTTTGAAGAGCGTTTAATGAAAATAATATGACTAAGGACCTAGATATAAACAATGTACCAAGCAACACACTAAAACACATTTTGGTGTAGAATTTAAATTAACTACTGAGACATGTGTGAGCAAGGCGCTCAAATCGATTTTAGTTCCATATTGTTGTACGAGGTTTCATTGAATTGAAACAAGGCAGTTGAATAGCCTAAACAAATATTTTCCTTTTATTTATAAATTATGTAATAAACAAAATATGCTTTTAGTGCGATCAAACGAT
VastDB Features
Vast-tools module Information
Secondary ID
FBgn0263490:FBtr0111012:5
Average complexity
IR
Mappability confidence:
NA
Protein Impact
Alternative protein isoforms
No structure available
Features
Disorder rate (Iupred):
C1=0.551 A=NA C2=0.747
Domain overlap (PFAM):
C1:
PF0009621=zf-C2H2=WD(100=9.8),PF121713=zf-C2H2_jaz=WD(100=8.9)
A:
NA
C2:
PF139121=zf-C2H2_6=PD(30.8=9.5)
Main Inclusion Isoform:
NA
Main Skipping Isoform:
FBpp0110311
Other Inclusion Isoforms:
FBpp0293452, FBpp0293453
Other Skipping Isoforms:
FBpp0293451, FBpp0293454
Associated events
Conservation
Human
(hg38)
No conservation detected
Human
(hg19)
No conservation detected
Mouse
(mm10)
No conservation detected
Mouse
(mm9)
No conservation detected
Rat
(rn6)
No conservation detected
Cow
(bosTau6)
No conservation detected
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AGCGCTGACAACAATGCCATA
R:
GCGTGGCTATTGTTGTTGCTG
Band lengths:
351-2159
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Neural diversity
- Neurogenesis
- Neuronal activity
- Splicing factor regulation (brain)
- Splicing factor regulation (SL2)