DmeINT0035822 @ dm6
Intron Retention
Gene
FBgn0051005 | qless
Description
The gene qless is referred to in FlyBase by the symbol Dmelqless (CG31005, FBgn0051005). It is a protein_coding_gene from Dmel. It has 3 annotated transcripts and 3 polypeptides (1 unique). Gene sequence location is 3R:31236035..31252608. Its molecular function is described by: trans-hexaprenyltranstransferase activity; prenyltransferase activity. It is involved in the biological process described with: response to mitochondrial depolarisation; ubiquinone biosynthetic process; neuroblast development; isoprenoid biosynthetic process; negative regulation of apoptotic process. 16 alleles are reported. The phenotype of these alleles manifest in: larval neuroblast. The phenotypic classes of alleles include: locomotor behavior defective; cell death defective; phenotype; neuroanatomy defective. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of low expression. Peak expression observed within 12-18 embryonic stages.
Coordinates
chr3R:31241635-31242310:-
Coord C1 exon
chr3R:31242171-31242310
Coord A exon
chr3R:31241777-31242170
Coord C2 exon
chr3R:31241635-31241776
Length
394 bp
Sequences
Splice sites
5' ss Seq
ACAGTGAGT
5' ss Score
8.34
3' ss Seq
TCTTCCTATCGCCTTGACAGCCA
3' ss Score
7.07
Exon sequences
Seq C1 exon
CTGCTGAAATCGGGCACATCCCAGCCAGAGTTGGACACCATTGCCAGCTATTACTTCGATGGCCAGGGCAAGGCTCTGCGACCCATGGTTACCATGCTGATGGCCAAGGCGATAAACTATCACCTGAACAACGAGTCACA
Seq A exon
GTGAGTACTAGTAGATGTGACTAATTAATATCTACATGAGAAGAGCTTACAAGCTTAGTAATGGTAGTCGTAAATAAATACTGCCTCTGTTTTTGATTTTATTATTTTCAAAAAGAACTCGTGGTTATTACCTAACGTAAAAGTATTCACATTTCTAACGAACTTTCCTGTTTTTTTTTTTAAGTTTAGTAGAGTTTAAAATGTTGCTATTAGTTTCTGTCATTTATCTGTGTAGACGAGTGTAATTGATTAGCACTGTTTTGCGAAATGTGCGCAGCCCGACCAAATGAAATCACACATACCCCTGCAAACACTTACATACATATGTATGTAGGTGTAGTTTTTGGATATTTGCCCTAGTCCTAAATCATGTATCTTCCTATCGCCTTGACAG
Seq C2 exon
CCAATTAGTACACAAACAGCGACAGATCGCCCTCTTTTCGGAGATGGTGCACTCGGCCAGTCTGGTCCACGACGATGTCATCGATCAGTCGGACTTCCGACGCGGCAAGCCCAGCGTGAATGCTCTGTGGAACCACAAGAAG
VastDB Features
Vast-tools module Information
Secondary ID
FBgn0051005:FBtr0085769:3
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0034812=polyprenyl_synt=PU(13.7=76.6)
A:
NA
C2:
PF0034812=polyprenyl_synt=FE(17.9=100)
Main Inclusion Isoform:
NA
Main Skipping Isoform:
FBpp0085131
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
FBpp0311406
Associated events
Conservation
Human
(hg38)
No conservation detected
Human
(hg19)
No conservation detected
Mouse
(mm10)
No conservation detected
Mouse
(mm9)
No conservation detected
Rat
(rn6)
No conservation detected
Cow
(bosTau6)
No conservation detected
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CTGCTGAAATCGGGCACATCC
R:
TTCTTGTGGTTCCACAGAGCA
Band lengths:
281-675
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Neural diversity
- Neurogenesis
- Neuronal activity
- Splicing factor regulation (brain)
- Splicing factor regulation (SL2)