DmeINT0036505 @ dm6
Intron Retention
Gene
FBgn0003392 | shi
Description
The gene shibire is referred to in FlyBase by the symbol Dmelshi (CG18102, FBgn0003392). It is a protein_coding_gene from Dmel. It has 16 annotated transcripts and 16 polypeptides (7 unique). Gene sequence location is X:15892116..15906716. Its molecular function is described by: GTP binding; actin binding; microtubule binding; GTPase activity. It is involved in the biological process described with 49 unique terms, many of which group under: mitotic cell cycle; synaptic signaling; organelle fission; cell motility; cell communication. 104 alleles are reported. The phenotypes of these alleles manifest in: cellular anatomical entity; apical plasma membrane; pacemaker neuron; cell division site; neuromuscular junction. The phenotypic classes of alleles include: behavior defective; sensory perception defective; phenotype; increased mortality. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of moderate expression. Peak expression observed at stages throughout embryogenesis, at stages throughout the larval period, at stages throughout the pupal period, in stages of adults of both sexes.
Coordinates
chrX:15898955-15901747:+
Coord C1 exon
chrX:15898955-15899074
Coord A exon
chrX:15899075-15901543
Coord C2 exon
chrX:15901544-15901747
Length
2469 bp
Sequences
Splice sites
5' ss Seq
GAGGTGCGT
5' ss Score
8.42
3' ss Seq
CCTATACGAATACTAAACAGTCG
3' ss Score
1.38
Exon sequences
Seq C1 exon
GACTACAAACAACTGGAGCTCTCGTGCGAGACTGTGGAGGATGTGGAGTCCTGGAAAGCATCCTTCCTGCGAGCTGGTGTCTATCCCGAAAAGCAGGAGACTCAGGAAAACGGCGATGAG
Seq A exon
GTGCGTACATAGATAGTTTACTAGTAATCCCTAGATCTAAGGCCAAACTCGATACTAATTGGATTTAGAAAAGAAGCGCTACACGAAGTAGTGGCTTTATAGGAATATTATATATTATTATTATTATATATATAATATATATTATTATTATATTATATGGCGTTTTATAGCTTTCCAAAAAAGCTGAAATTTTTAAGGTTGTATTTTAAGATGGCATAGTTGTCGTTTAGATATTTCCACTTAAATTGAAAGCATTTCCGATTACTACATTATCTATATATTTATATATCTTTGTTTCCGCTGTTTCATTGACCAAATCAACAAAATAACTCGCAATATCGATGCTTTAAATGTGCATTCGTCATCCAGGAAGGACAAGAGGTTTGTTAATCTTTGCATCTTTTCGGAATATATACATATATATTGTTTAGTAAACTCATCAATTAAATTTCGTATAAATGTATGCGATTTTGAATACTAATAACTAATACATTATTTTTAACATACTTTTGATCATTTATCTTTCGTTTAATTTCGGTTGTTTCATTTTTAAAGTCTGGTGAGGTATTTTAGCCGTTATTAGATACAAATTGTTTTTATTATTAAAAAATAGAAATTCCAAATTTTAAACTACTAACATATTGTTAATCGGCTGAGGATTTGCTAAAATTAAATAATAACTGCGGCTTTAAATGTCATCACATTACAAAAAATCATACATATGAGCTAGTACACCTCGAAGTCTCAAGATGGTGTAACCCCTTAAATGGTTAAAGGTGCGATAAATTACCATATCACAAGCCGAGTATTACAAATTGGATATATATATATATAAATTCGTTTACGATTGTGTTAAAATTGAAGAGTTTTGATCAAAGTTTTCCACTTAAATTGAATTCATTCCACTCGGCTTGTGATACATTTCTTTTCATCAGCTAAAGTTGTAGTTTGTCGGTCTTCCATCTTGTTTATTTAACTAACTTAGAATTACTGATTAAGATAGTCGGCTGCTCGACTATGGCATTGTTACTGGTTTCTTATAACGATTGTGAACATGTTGTTCTCTTCTATTTTTCCGCCACCTACCAAACAAAAAAAAAAAAAAAAAAACGACCAATCTATCTATGAAACGCCAAAATACACACAAAAATTAATAATTTCTTCCGGTTGGTTTACTTAGCAAAAAGTATGTTGATTTCAATTGTTTATATTGGTTTGATTTTTTTCCTTATTAAATTGTTGCATTTTCTCACGATATAATTATTCATTTATACCCGCTACTGAAATTACTCGTAATTATTTCTAAGATATATCCATGTCCTCCGTATATCTGAATAATCGTTGAGATAAAAACTAAGTTAGTAGTTGGGCTTGATTTGTTTTCATTTGTTTGAAACGGGTTAACAATAGCGGGTATCACATAGTCGGATATTCGGCTCTATTGTTCCTCCTTGATTTTAATCAATTTCAAGATGACTTGTCTTCCATTTAAGTAAATAAAAGTTATAGATATACGTAAGGTGTATCTATATTGTGATCCATATTCGAATTTTACTTAAATTTTTGACAGTCGTCGAAAGCATGCACTTTGTACTTTTCAAGTTAATTTGTATTATATTTTACTATTGGATTAATTTATTTCACTCAACGTTCGAAATCAATATATTCAAACGCATGCCATTCGAATTCAAAGATGAGAGTTCGAAAACGCAATCATATTTATGTAAATGTTATGAGTTATATAAGTTTGAGTTTATATAAGATTGGGGCCTAGAAACTGTAACATCTGCAGTATTTTTCTAGTTTCTAGTTGGTTTATCGTGTGTGAAAATGTTGGATTTTCAATCGCTCAAAAGTTTTAAATGCTGGCGAGGTCTAAGAATGGCCATGGTATCAGTATAGTTTTTTTTAGTTAATGCTCCCTATTCAGTAATTGCAATAATCCCAATATTCAGAATCGAAAAAGGTGGCATCACTGAAATAAATTTCATTTCCCTTAATGTTCAAATATTTTGAAAAAGAAATAGAATATTTTAGACTGTTGAGGGGTTACGCCACCCTAGAAAATGAAAAACTTGAAACGATTTTTTAAAATTGTGCTAAAACGAATGATGCATTCATTCTATCGAAAACTTTTACTTTTTTCCTCCGCCGCCAGCAAATTTATAATATGCATGTTTTTCCTTTTTTTTTGTGAAGAAAGCCACGCGCGAATTCTTTTTGGTTACGCAACTTACTTTGAGTGTGGGAATTGGGTAAAGGGTATCTTATAGTCGGACCCTCGACTGCAGCGTGTTCTTTCTCGTTTTTATGTGCACGATTCAGTTGGTTATTTTGCTTTCAAACACTTATTCGGTTGGCTATTAGGTTTTTTTTTTAAATACTTATATGCACTCTATAAAATTACGAACAATCATTTATGGACTATCAATATCCCAAAACCCCCAACCCTATACGAATACTAAACAG
Seq C2 exon
TCGGCCAGCGAGGAGAGTTCCAGCGATCCACAGCTGGAGCGGCAGGTCGAGACCATTCGTAATCTGGTTGATTCCTACATGAAGATCGTTACGAAGACCACCCGTGACATGGTGCCCAAAGCAATTATGATGCTGATCATCAACAATGCCAAGGACTTTATCAATGGCGAGCTGCTGGCACATCTTTACGCGTCCGGCGATCAG
VastDB Features
Vast-tools module Information
Secondary ID
FBgn0003392:FBtr0074121:10
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.300 A=NA C2=0.283
Domain overlap (PFAM):
C1:
PF0016924=PH=PD(22.6=60.0)
A:
NA
C2:
PF0221213=GED=PU(62.0=83.8)
Main Inclusion Isoform:
NA
Main Skipping Isoform:
FBpp0305866
Other Inclusion Isoforms:
FBpp0290811, FBpp0290812, FBpp0305867, FBpp0309359
Other Skipping Isoforms:
FBpp0073928, FBpp0073929, FBpp0089277, FBpp0089278, FBpp0089279, FBpp0089280, FBpp0110335, FBpp0110336, FBpp0290809, FBpp0290810
Associated events
Conservation
Human
(hg38)
No conservation detected
Human
(hg19)
No conservation detected
Mouse
(mm10)
No conservation detected
Mouse
(mm9)
No conservation detected
Rat
(rn6)
No conservation detected
Cow
(bosTau6)
No conservation detected
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
No suggested primer sequences
R:
No suggested primer sequences
Band lengths:
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Neural diversity
- Neurogenesis
- Neuronal activity
- Splicing factor regulation (brain)
- Splicing factor regulation (SL2)