DmeINT0037129 @ dm6
Intron Retention
Gene
FBgn0002466 | sti
Description
The gene sticky is referred to in FlyBase by the symbol Dmelsti (CG10522, FBgn0002466). It is a protein_coding_gene from Dmel. It has 3 annotated transcripts and 3 polypeptides (2 unique). Gene sequence location is 3L:12507998..12514815. Its molecular function is described by: ATP binding; protein serine/threonine kinase activity. It is involved in the biological process described with 12 unique terms, many of which group under: nervous system development; chromosome organization; regulation of cellular component biogenesis; metabolic process; central nervous system development. 29 alleles are reported. The phenotypes of these alleles manifest in: imaginal tissue; male-specific anatomical entity; follicle cell; intracellular organelle; spermatocyte. The phenotypic classes of alleles include: increased mortality; phenotype; sterile; modifier of variegation. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of very low expression. Peak expression observed within 00-06 hour embryonic stages.
Coordinates
chr3L:12510029-12511803:-
Coord C1 exon
chr3L:12510301-12511803
Coord A exon
chr3L:12510225-12510300
Coord C2 exon
chr3L:12510029-12510224
Length
76 bp
Sequences
Splice sites
5' ss Seq
CAGGTATAG
5' ss Score
8.73
3' ss Seq
TTTTTGTATTTATATTTTAGCTC
3' ss Score
8.92
Exon sequences
Seq C1 exon
CTTGCCGAATGTCGCTGTCAGCTGGCTGAATCCTCACTAGCCACCCAGGTTTCCCCCTACGAAACTGCGCCTGGATCTTTGACCGAACTGAATGCCATTGAGGATCAACTTCGCGCCGACTTGTTAGCCGCCAAAGAGAGTGAAAACCATCAGAAGGGACGCGCCGATCAACTGCAGACGCTGGTTACCAAACTGGAGCAAATGCTGGAGCGCTTCAACGAGCAGAGTCTTTCACCCACGAAATCGCACTCATCGCGTAAGCAAGAAGGTGAAACCGTTGGTGATATGCTCGAGCGGCAAAACGAGAAGCTGGAGGATAAATTGGCAGCAGTGCGAGAGCAGATGATTGTGGAACGTCAGGCAGCGCGTACAGCGAATCTATCGCTCTGGAAGGTGGAGAAACAGCTCGAGGAAGCTTTGTCCGAGAAGAAGTTACTGGCGCGTCGCATGGAACTCACAGAGGATCGGATTAAAAAGGTGCAGAACGCAAGCGATGAAGCGCAGAGAATGCTCAAGACGTCACAGGAGGAAACTCGCCAGCGGGAGTCTCGCATCGAGGAGCTGAAGCAGGAACTGGCGGCCGCCAAAAGGGATGTCCTCAAGGAGCACCGCCAATGGGAAAAGGCCGAGCAGGAGCGCATGAAGTGCAAGTCGGAGATAATCGAGCACTTGGCCAATGTGCACAGATTAGAACAACAGGAGACGGAATTGCGCCAGAAACTCAGGCAAATCCAATCGCGATTTGATGGTGTAACTTTGGAACAGAAGAATACCATTCGAGAACTGCAGGAGGAGCGAGAAAAGAGCAGGAAAGCGAATGACTCTTGTTTGGTCTTACAGAAGGAGCTGAAACAACTCACCGATAACTTCCAGAGGTTGAAGTACGCTTGTAGCATTACCGACAGCCAGCTAACTGAGGTGGAGACCATGCTAAAATCGGAGCAGGAACGAAATAAATCCCAGAAATCTCAACTTGATACCCTTCATGAGAAACTCAGAGAGCGCAACGATCAATTAACTGATCTACGAAAGCAACTAACTACGGTGGAATCGGAAAAGCGTCTGGCGGAGCAGCGTGCTCAAGTGTTGGCATCGGAAATCGATGAACTTCGTCTCAATCTCAAGGAGCAGCAGAAGAAGCTGGTGGCGCAGCAGGATCAGTTGGTGGAGCAGACTAACGCACTGTTTGCCACCCAAGAGAGAGCTGAACTTTTGGATGGGCAAAATGCCAATTACGAAGCCCAAACTGCGGATTCGAATCGCGAAATGGTTAGTTTGAAGGAGGAAAATGCTAGAATTCTCAGCGAGCTGTTCCACAAGAAGGAGGAGGTTGGCAATCTTCAGGCGGAGATAAGGGGCTTGGAGTCTGCGCAAGCCAACTTGCACGCAGAGATCGATTCCCTTCAGGACACTTTGGCCGAGAAGGAGCAGTTCTACGTGCAGCGGGACATTAAATCGAATGCCACACTTGCGCAGCACAAAAAGCTGATTGACTACCTTCAG
Seq A exon
GTATAGCTAGTTTATATATATTCTTTTTAAGAGAATAGTTAATGAGATTAAAAACATTTTTGTATTTATATTTTAG
Seq C2 exon
CTCAAAGTTGAGGACCTATCCGCCAAGAAGAAAAAGACATTGGCGGACAAACTTTTCGGTTCTAGCCACACAAACAAGGAGAACGTGTCACCCAATGACGTGGAATCTTCCATTCTGTATCGCGCTCTAAAGGAGGAACTGAAGCGAGAACAAAAGATGAACAGCTTGCTGAAGGAGCAGTTGGCTCAGTTGAATG
VastDB Features
Vast-tools module Information
Secondary ID
FBgn0002466:FBtr0075970:5
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.381 A=NA C2=0.470
Domain overlap (PFAM):
C1:
NO
A:
NA
C2:
NO
Main Inclusion Isoform:
NA
Main Skipping Isoform:
FBpp0304889
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
FBpp0075702
Associated events
Conservation
Human
(hg38)
No conservation detected
Human
(hg19)
No conservation detected
Mouse
(mm10)
No conservation detected
Mouse
(mm9)
No conservation detected
Rat
(rn6)
No conservation detected
Cow
(bosTau6)
No conservation detected
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TTGCACGCAGAGATCGATTCC
R:
ACCGAAAAGTTTGTCCGCCAA
Band lengths:
183-259
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Neural diversity
- Neurogenesis
- Neuronal activity
- Splicing factor regulation (brain)
- Splicing factor regulation (SL2)