DmeINT0037604 @ dm6
Intron Retention
Gene
FBgn0033636 | tou
Description
The gene toutatis is referred to in FlyBase by the symbol Dmel ou (CG10897, FBgn0033636). It is a protein_coding_gene from Dmel. It has 6 annotated transcripts and 6 polypeptides (5 unique). Gene sequence location is 2R:11577485..11616045. Its molecular function is described by: protein binding; transcription factor binding; DNA binding. It is involved in the biological process described with: ATP-dependent chromatin remodeling; nervous system development; positive regulation of transcription by RNA polymerase II. 67 alleles are reported. The phenotypes of these alleles manifest in: trichogen cell; wing; dorsocentral bristle; wing vein L5; scutellar bristle. The phenotypic classes of alleles include: fertile; visible; partially lethal; lethal; viable. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-06 hour embryonic stages.
Coordinates
chr2R:11581628-11587025:-
Coord C1 exon
chr2R:11583015-11587025
Coord A exon
chr2R:11581750-11583014
Coord C2 exon
chr2R:11581628-11581749
Length
1265 bp
Sequences
Splice sites
5' ss Seq
GCGGTAAGA
5' ss Score
9.63
3' ss Seq
AATATTCCAATTCCTTACAGAAT
3' ss Score
7.59
Exon sequences
Seq C1 exon
GAACGAGAACGTCGCCGCCAACACATGAGCCTCATTCGAATGTTGGAGCTTCGTCGGAAATTCGAGGATCGTGAAAAGAAGAAACACCAGCTGGTTCTAGACCGTTTGCTTCTGCGTGAGCGTCGTATGGCGGAGCGAAAACGAGATGCGGAGATTCTGCAGTTGATAAGGCGTCCCAATGAGGACTCCGAAATGCCACAAGAATTGGTAATCCCTGAACTGGATAGGATTGCCGGCAACCGACTTCCCGGCCAGGCAATGGCCGATTTGCTCATGGTCTTTGAATTCCTACATAACTTTGGGGAGACTCTAGGCTTTGACATGGAATCACTGCCGTCGCTTCAGAACCTGCACGATGCTTTAATGAGCGATAGCAACGCGGATGCGGAAGAGGAGTTGTTGTCGGTGATGACGCATCTATTGGTTTGTGCCATTGAGGATCCAGGTGTTCCCAATCCCGGCAGACACACCACTTTACTGGGACAATCGCTACGCAATGCGGACATCACCAACTCGAATGTATCCGAAATCTTGAGGATTTATCTGTATGCTACGGCCACAGGTGAAGTGCGTCAAATGCACGGAATCACTGTGGATCGTGAGCGAGAGAGAAGGGTGCCGGATCATCATCAACTAGATAGCGATACCACAACCCACTCACATTCGGTAAAGAACCAGGAGTACTATAAGCTTCTCCATGAAAACGACACCTGGAAGTTGTCGCAATCGCTGAAAGATCGCCCCTTCGTTGCGTTAAATCCCACCCGTAAAGCCCAAATGTTGGCCCATCTATGCAACGACCTGCTCATGAACAAGGCCGTGCTCCGCCAGATTGATGGAAGTCTAGAAACGTGCGCCCAGATGCGAAAGGAGAAATACATGACGGACATGAAGGTGCGCAAGTACAAGGCTCTCCACATGCGCAAGGCTCGAATTGAGGCCTATGAACGAGCACAGGCGGAGCGTGAGGCGGCTATGCAGGCGTTGATGGCACAGCAGAAGCTGGATGCAGAACGTCTTAAGGCGGAGGAGGAAGCCAAGGCCGCGGCGGCAGAGGAAGCAGCAGCTGCAGCGGGCACAGATGGAGAGGCAACCAAAGGTGGCTCACCCAATGGCGAGAAGCCGGAAGACGGCGATCAGAATGAAGAGGGAGCCGCCAAGGAACCCCAGCAGCAGCAACAGCAGCCAATGGAAGTGGATGGCGTGGTCGATGAGGCATCTCTTGTAAGTCCGGCCAAAACCATCATTCAAACGGACAATAGTCTGACGCCCAGTAAACAGGACATGCCCACACCCACCTACCAAATTAATGGATCCAGCACACCTACCACAAGTGGTGTCACTGGAGGCGACATGAATGTTCTGCTGCAGGCCAAGAAAAGCGGGGCCCGAAACTCCATCAACGATGAACACCACCATGATGTTAGTATTATCGACGATGATCTTTCCGACTTGGATTCGGAGATCACGAATGTCGAGGAGGACGAGGACAATCGCTTGAGTGCCGATGAGTTGCAAAAGAAGCTCGACAAGATTGTCAGAGCTTCCCTAAACTGCAAGGAGGCACTGGAGAAGAGCACAAATCAGTTGAGAGCTGCGTGCTTTGGGCAGGATCGCTTCTGGCGTCGCTACTGGAAGTTGCCCAAGGCTGGAGGTATTTTCATTGAAGCACTTGAGTCGGCCCAAAATGATATTTGCGATTATCATGAGGCATTGGAGGCCATGGATGACAAAAAGGATGCAAACGACGAGAAAGAGAACTCCGAAAATGAGAAGGATGTTGCAGCGGAGTCCAGTGAGCAGCCAATGGAAGTGGATGAGTCCATTACAAAACTGGAAGATGGTGTACCAGCTTCGGACGTCGGGATGCCTGAAAGTAATCAACAGAACGCACATCAAGATGAGGAAGATGACGATGATGATGTGACAGAAATCAACAAGGTGGAACCGGAAATTGTTGATTTGGGTGATGATGACGATGATGCAGCTCCCCCGCTGCCCAAGATAGAACCTCCCAGGCCAGAGATTAAAGTTAAATCAGAGATGGAGCTGATGGGTCCACCACCCACGACTATGATATCCACTAAGACAGACTTTGAAGCTGAAATTAAAATACCCTCTATGCCTGGCATCTTGATGCCGCCTACCCTCAACAACAACAACACCAATAATAACAATAACAACAATGGAAGCGACAACTGTGATAAGTTGGAAACTGGTTTGGGACTAGGACAGCAGCAGCAGAACTTTAGCCAGTCTGTGATCAAGACGGAGGATGTGAAAAAAGAGGATGATTGCATAATAGTTTCTACATCCAGTGTTGACGATACACCAAAGTGGTTCTCCATTGTCCGGCGGGAGGTTCCATTGATCAGCGAACTGCCAGCGGAGGAGGGAGGCGTGGTGGGCCAGGAACTTCAGATCAGCTATGCAAATCAGAACTGCTCCGCTCAGCTGCAGCTGCAAGGTCATCCGTGGGATCTGATCAACAACATGCAGTACTACTCGATTCCGATGGACGAATGCAAGGTGGACACCAGCAAGCTGGGCAACGAGTGCATCTTCTCGCTGAGTGGCCTCGATGAAAAGCAAATGCTAGCCAAGGTGGAGGAGTACAAGGCCCACAAGGTGGAGTCAAAAAACGGTCTGGGCTCTCCTCATCGCCACCACGAGACTAAAGATGATGAGGAGCAGGCCAAGCTGAAGTTGGACAAGGAGATTGACACCGAAATGGAAACAGATGCAGATGACCTAGCTGGCAAGGAAAAGTTTTTTCGCTTGCGCTCTGATGTACCCCCGGATACGGGAGGAGGGGTCAGCGAGGGAACCGATGTAAAGCCCAAGATTGAGCTTCGTTTGGATGAGGCGTTGTCACAGGCATATTACCACAACATAGCCAACATGTCATTGAGCAGTGTTCAAACGTATATACCCATCGATATCCCTCTGCCGCTTTCCATGACCCCCGATGAGCATCGTCTGCTGGAGCAGGTCAAGCTGGCAGGTTTTCCGGAACGAGTTCACGGAGTTTACGTGCCCCGCAGACAGCGCTACGGCTGGTGGCAGTTGGACGATGAACAGAAGCTGCGCCAGCTGCTCAAAACTCTCAATCCGTCGGGATTGAGGGAGCGTGAACTGCAGGAGAATCTTCAACGATTCCTCGGACTGGAACAGCCATTGGGTGTGAATTATAAGCTGAAATCGGACATCGATTTTCCAGAGGAATTCCTAATGCCTGACAAGAAGGGCGATTGGAATCCCAAGGTGGCCAAACGTGTGGAGCTTGCCCTTATCGAACAACTAGAATCACTGGAGGACAAGGTGGCCAGCGCTTCTATGCAATTAAAGAACTGGCAATTGCCCAACCGCGTGGAGAGTGAACTCACCTTGGACTCGCAGGAGGATGTCACCGAAGAGGACTTCGTCAGCATCATACCCATGATCCGAGAGAGAATCATCGACTTGGAGGCAAATATCGAAAGACGTTACTTGAAGCCGCCTTTGGGCTCGCAGACAGGCGATGCCCATTTGGCGGTTATTGCTCAGAACCAGCATACCACCACCCAGACGCAGAACTCTGCATCGGCAGCAGCATATCTCCTGCAGATGCAACAGCAGCAGCAGCAACAGCAGTTGGCCCAGCAGCAGCAGCAACAGCAACAGGGTTCGGGTGCGGGAAATAGCCTGAATCCCTCATCCTTCAACGAGCGTACTATGGCATTGGCGGCGGCAGCAGCTGCTTCGGGGCCAGGAAACGCAACCGGAGTAGCCAACTCGGCAGTCGTGGCAGGAGCCACGCCCTGCGAATCGGGCAGCGGAGAACCGAATTCCGGCAACGCGTCACCGGCCAGCAACTGTGACAGCGATCGGGACGAGAAGGTGGAGCAGATACCCAAGGGATTGGTGCAGTGGCGGGACGCAGTATCCCGATCGCACACCACCGCCCAGCTGGCGATGGCCCTGTACGTTCTGGAATCCTGCGTGGCTTGGGACAAGAGCATTATGAAAGCG
Seq A exon
GTAAGATTAGGAGGATTTTGAATACCTTTAAAATTTCCCGATTTGAAAACGATAACCTGCTTGGCACCCGCCTATATTTGTGGTCCATTTCATTGTTGTTTTGGCTGCACACACACTAACACCCACTACTCACAACCCACATACATAATTGTACACACATGCATAGCATTACCATCTAATATTTCGACACACTAACCGGTATTCACCACAGCTGAAACGGAGGTTTTGAAACTTTAATGAAGAGAGCTTGTATCACGGTTGTTTTACGAATTTAATATTTTCGAACTTTTACTTTTCAATATAACGTTATGCTTTTTAATCAAATTATCTTATATTGAAGTAGATTTAAAAAATAGTAATATAAGAATAATAAGTCACTTGGAGTTATATTTTTATATAGTATTAGTAACTTTTGTTTAAATCACTTAAAGAATGCGGTTTTTTAAAAGCAACTGTGATTACACTGCTCATTCGATTTATTTTGTATGTAGTCGAGTAGTTTTTAGAAACCCCCTCTGCATACAGATACGTGGAAATTGCCTGTGAAATTAATTCGAATTTTGTTTTGTTTCTTCTTCCGATTTTGTTTTTTCTTCTTAACTGTCTCTTGCTGAATGTTTGACTTGTTTGTTGCTTGTGGTGTTTCATGGTGTTCTTTAAACAAAATACAACAAATTTAACGCTTTCATACTCACACACACACACAAATACATTATCCACCACTCACACAAACATGTACATGAATGCTTTGGTTGCCCATTACCGTTTACCGATAACCGTTAAACCCCTTACAAACGAAACCCACCGAACCCACCACGACTGTTAATCCCACCCCTCAAACGAACCGTTTCGAACTGCCATCCGAAAAGTATGCAACAAAAAACAAGTCCAGCAAAAAGAAGAGCAGCGCCAAAAAGCAGGCAACTCCCTCGAAGAAACAGCAGCAGAAGAAAAACAAAAAGGAGCAGCAGTTGACCCCCAATGGAAAAGAGAGCAAGAGCAAGAGCGCAATCAATAAGCCAGAAAACCAGGCTCCGCTGAGCATCAAAATCAACCTTAAGGCTCTGGCCCAAAACGGGCAGTGTTTGCTGAAAACTAAAACACCACCAATCCTAACCAAATCCCCAACCGCATCCCCATCCTCCCATCCAAACACTAGCGACTCCGACTCGGACTTTGGCAAACGGACGAAGAAGAAGAGCGGCGGCAAACGCAGACGCTCGGCCAACAATACAAACTCTAGCAAATATTCCAATTCCTTACAG
Seq C2 exon
AATTGCCAGTTCTGCACGTCCGGCGAAAACGAGGACAAGTTGCTACTGTGCGACGGCTGTGACAAGGGCTATCACACCTATTGCTTCAAGCCCAAGATGGACAACATTCCCGATGGCGATTG
VastDB Features
Vast-tools module Information
Secondary ID
FBgn0033636:FBtr0088091:12
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.504 A=NA C2=0.505
Domain overlap (PFAM):
C1:
PF0279112=DDT=WD(100=4.8),PF156121=WHIM1=WD(100=3.7),PF156131=WHIM2=WD(100=4.3),PF156141=WHIM3=WD(100=3.1)
A:
NA
C2:
PF0062824=PHD=PU(81.6=97.6)
Main Inclusion Isoform:
NA
Main Skipping Isoform:
FBpp0087193
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Human
(hg38)
No conservation detected
Human
(hg19)
No conservation detected
Mouse
(mm10)
No conservation detected
Mouse
(mm9)
No conservation detected
Rat
(rn6)
No conservation detected
Cow
(bosTau6)
No conservation detected
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ACGAGCGTACTATGGCATTGG
R:
GCAACTTGTCCTCGTTTTCGC
Band lengths:
351-1616
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Neural diversity
- Neurogenesis
- Neuronal activity
- Splicing factor regulation (brain)
- Splicing factor regulation (SL2)