DmeINT0037606 @ dm6
Intron Retention
Gene
FBgn0033636 | tou
Description
The gene toutatis is referred to in FlyBase by the symbol Dmel ou (CG10897, FBgn0033636). It is a protein_coding_gene from Dmel. It has 6 annotated transcripts and 6 polypeptides (5 unique). Gene sequence location is 2R:11577485..11616045. Its molecular function is described by: protein binding; transcription factor binding; DNA binding. It is involved in the biological process described with: ATP-dependent chromatin remodeling; nervous system development; positive regulation of transcription by RNA polymerase II. 67 alleles are reported. The phenotypes of these alleles manifest in: trichogen cell; wing; dorsocentral bristle; wing vein L5; scutellar bristle. The phenotypic classes of alleles include: fertile; visible; partially lethal; lethal; viable. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-06 hour embryonic stages.
Coordinates
chr2R:11580235-11581398:-
Coord C1 exon
chr2R:11580949-11581398
Coord A exon
chr2R:11580863-11580948
Coord C2 exon
chr2R:11580235-11580862
Length
86 bp
Sequences
Splice sites
5' ss Seq
GAGGTGAGT
5' ss Score
10.03
3' ss Seq
AATGCCTCTGCTCTCTGCAGTCC
3' ss Score
8.53
Exon sequences
Seq C1 exon
GTACTGCTACGAGTGCGTGAACAAGGCCACCAATGAGCGCAAGTGCATCGTTTGCGGCGGTCATCGTCCGTCGCCCGTGGGGAAGATGATCTACTGTGACTTGTGTCCTCGTGCCTACCACGCCGATTGCTATATACCGCCGCTGCTAAAGGTACCGCGTGGCAAGTGGTACTGCCACGGTTGCATCTCCCGGGCGCCTCCGCCGAAGAAAAGAAGTGCAGGTGGAACATCCGGCAGCAGCAGCAAATCTAGGAGGGACAGGGATAGGGAGTCGGGCGGATCGGCCAAGCGGCGTAGCGACAACAGCAAGACACCAGCCATGGAGCACATGCAGCAGCAGCAGATGCCACTGGCAGGCGGGGATTCCCACCATCATCATCATCAGCAGCCGCCGTCGCTGAACTCCTCGCACGATGAGTCGATGAATTCTCTGCCGGCGGCTCCTCTGAG
Seq A exon
GTGAGTGTGGGGGCCTGCGGGCGGCTGAATGGGCGGCGGAGTACACGGTCCGGGTTCCAGTTAAATAATGCCTCTGCTCTCTGCAG
Seq C2 exon
TCCGGCGCACTCGGTGGTCTCGGCCACGAACTACGATGACCAGCACCATGCCAACAACTCGGTCGATGGCAGCAGTCGCTTCCACGCGCATCTCATTCCCCCGTCAAATAATGGCACTGCGGCTCTGCTGGAAGATGTGCCGGGTGGCGCCAATGTGATGCCCGGCGTCTATCCAGTTTATACGCCAGTTGCGGCTGGCAACTTCTCAGCCGGATTGATTAACCAAGCGCCGGTGCAGCCAGCGATGCCGTTTGCCAACGTGGTCGCCATGTCGCCACGGGCTGTCACGCCCACCCGCACCCGAACGCCCACACCGACGCCAGCACCCACTCCGCCACCACCACCGCCGACACCGCTGCTAATGCAGGCCTCGCCCACAGCCACCGCCCTCCATGTAAACGCCTGCCAGTCACCGCCCCAACAACAGCATGCGCAGCTGATGACCATGCCCTCGCCACCAGCCATTGGAGTCGGAACGGCCACTAACCAAATGTCGCCACCGCCCATCAACATACATGCCATTCAGGAAGCCAAGGAGAAGCTGAAGCAGGAGAAGAAGGAGAAGCACGCCACCAAGAAGCTCATGAAGGAGCTGGCTGTCTGCAAGACGCTATTGGGGGAAATGGAG
VastDB Features
Vast-tools module Information
Secondary ID
FBgn0033636:FBtr0088091:14
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.559 A=NA C2=0.819
Domain overlap (PFAM):
C1:
PF0062824=PHD=PD(16.3=5.3),PF0062824=PHD=WD(100=33.8)
A:
NA
C2:
PF0043920=Bromodomain=PU(9.6=3.8)
Main Inclusion Isoform:
FBpp0087196
Main Skipping Isoform:
FBpp0111979
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
FBpp0087193, FBpp0305283, FBpp0305284
Associated events
Conservation
Human
(hg38)
No conservation detected
Human
(hg19)
No conservation detected
Mouse
(mm10)
No conservation detected
Mouse
(mm9)
No conservation detected
Rat
(rn6)
No conservation detected
Cow
(bosTau6)
No conservation detected
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CTGAACTCCTCGCACGATGAG
R:
AGCCGCAGTGCCATTATTTGA
Band lengths:
177-263
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Neural diversity
- Neurogenesis
- Neuronal activity
- Splicing factor regulation (brain)
- Splicing factor regulation (SL2)