DreALTD0013740-3/3 @ danRer10
Alternative 5'ss
Gene
ENSDARG00000099791 | pdk3b
Description
pyruvate dehydrogenase kinase, isozyme 3b [Source:ZFIN;Acc:ZDB-GENE-070112-1892]
Coordinates
chr21:40571780-40575679:+
Coord C1 exon
chr21:40571780-40571921
Coord A exon
chr21:40571922-40571934
Coord C2 exon
chr21:40575608-40575679
Length
13 bp
Sequences
Splice sites
5' ss Seq
CAGGTGTGA
5' ss Score
5.29
3' ss Seq
TGTCTTTTCTGTGATATCAGGTA
3' ss Score
6.3
Exon sequences
Seq C1 exon
GCAGGGAAAACGCATGTGAAAAAACCTCCTACATGTTTCTGCGGAAAGAACTGGCGGTGAGGTTGGCCAATACGATGAGGGAAGTCACCTTGTTGCCCGACAGTCTCCAGATCCAGCCGTCTGTCAAACTGGTGGAGTCCTG
Seq A exon
GTGAGGAAGGCAG
Seq C2 exon
GTACAGCCAGAGCTTTGAAGAGCTGTTAAAGTTTGAGAAAAGAAGTCCAGAGGATCCTCACACACTTAATGA
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000099791-1-3,2-3,3-3-3/3
Average complexity
Alt5
Mappability confidence:
NA
Protein Impact
ORF disruption when splice site is used (sequence inclusion)
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF104364=BCDHK_Adom3=FE(28.5=100)
A:
NA
C2:
PF104364=BCDHK_Adom3=FE(14.5=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Mouse
(mm10)
No conservation detected
Mouse
(mm9)
No conservation detected
Rat
(rn6)
No conservation detected
Cow
(bosTau6)
No conservation detected
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CCGTCTGTCAAACTGGTGGAG
R:
AGTGTGTGAGGATCCTCTGGA
Band lengths:
92-105
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]