DreEX0055881 @ danRer10
Exon Skipping
Gene
ENSDARG00000079585 | pkn3
Description
protein kinase N3 [Source:ZFIN;Acc:ZDB-GENE-030131-1902]
Coordinates
chr5:31248539-31252643:-
Coord C1 exon
chr5:31252489-31252643
Coord A exon
chr5:31251573-31252395
Coord C2 exon
chr5:31248539-31248668
Length
823 bp
Sequences
Splice sites
3' ss Seq
CCTCTGTGTATTTAAATCAGTTT
3' ss Score
5.59
5' ss Seq
ACGGTATAG
5' ss Score
5.27
Exon sequences
Seq C1 exon
ATGATGCCACCTCCCCAGACCCGTGCCATTGGGAAGAAGTCACATCACCCTTGGGCAGTCATGTCCGTACACTGAAAAAACAGCTTACCATGGAGCTCAAAGTGAAGCAAGGAGCTGAGAACATTATCCAGACTTACACAAACAGTTCTGTCAAG
Seq A exon
TTTTGGATGCTGCCAAATGGCTATGAAATATGATAATAAGCACACAAAGGAGGCCCCTCCCCCCGACCCTCATCTTTTATAACCCTTTTAAAGTGACGTCCTATAACCTCTAGCTAGCCATGAGTGTATTTTCCACTTATTCTTTGAGTCCCAGACATTGCTCTTATAATGCTGCGGCGTCTGGCACAGAACACAGTGTGCTGGCAGCACTGTGATTTATGTCTGTGCTGGATTCAGACAGCTTGAGTACCGAGCTTTGATCTCCCTCACACACACACACACTCACAAGCGCACACTAAAACATCAGCACCTTGGAGTCTGTGGAGGAAGAGCTTGCTGGTACTGATTCGGTTTCTCCTGTCCTGGGCCTGGCGACAGCAGCAGCCCATGTGACTTTGCTCCAACCAATACCAACGCATTCATGTTTGGGCCAGCCAGCAGCAGCAGCAGTTAGAGGTGGCGAACTCCTGTAGAAGAGAATCTGTAAACGGAGGGAGAGAGAGAGACGGGGAGAAGGAACAAATGAGAAAAGGGGAGGGGAGTAGTGAGTGAATGAGCGAGTGAGTGAGCGTTCAGGATGCAAGGACAGTAGAACAATAAGAAGAAAGAATGGGGTGGCTTCTCGGATGCTGATTCGTGATCAGCGTTCTGTGGGAGAAACTGACGAGAGCATGTCTGAGGACGGAGAGACGAACGCATCAGGAGGAAATGTCTGATGGGGGTCAAGGAAGAGCAAGGGGATGTCATTCAGCTATCTGAAAGTTCACAAAACATGACAGATGACCTCTTGAGCTGCATTGAGAGGGGGAGAGCGCTATGGACG
Seq C2 exon
GATCGGAAGATGTTGTCCACAGCCCAGCAGATGCTGCAGGATAGTCGGACGAAAATCGAGCTGCTCCGCATGCAGATTGTCAAAGTCACACAGGCCAGAGAAGGAGAGAGAGAGACCACACAGCAAGAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000079585-'6-4,'6-3,7-4
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.442 A=0.000 C2=0.545
Domain overlap (PFAM):
C1:
PF154561=Uds1=PU(48.8=75.0),PF0218511=HR1=PU(43.1=59.6)
A:
PF0218511=HR1=PD(2.9=9.1)
C2:
PF154561=Uds1=PD(48.8=88.6),PF0218511=HR1=PD(54.2=88.6)
Main Inclusion Isoform:
ENSDART00000112546fB4696
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Human
(hg38)
No conservation detected
Human
(hg19)
No conservation detected
Mouse
(mm10)
No conservation detected
Mouse
(mm9)
No conservation detected
Rat
(rn6)
No conservation detected
Cow
(bosTau6)
No conservation detected
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ATGATGCCACCTCCCCAGAC
R:
TGCTGTGTGGTCTCTCTCTCT
Band lengths:
280-1103
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]