DreEX0064358 @ danRer10
Exon Skipping
Gene
ENSDARG00000040274 | scamp5b
Description
secretory carrier membrane protein 5b [Source:ZFIN;Acc:ZDB-GENE-120215-76]
Coordinates
chr18:50094231-50096922:+
Coord C1 exon
chr18:50094231-50094350
Coord A exon
chr18:50095461-50095488
Coord C2 exon
chr18:50096766-50096922
Length
28 bp
Sequences
Splice sites
3' ss Seq
ATTGTCATCTTTTGCTGTAGATA
3' ss Score
8.77
5' ss Seq
AAGGTATTT
5' ss Score
7.64
Exon sequences
Seq C1 exon
AAAACAACTTTCCACCTCTGCCGGGCTTCATTCCTCTCAAGCCGTGTTTCCACCAGGACTTTGAGGAGATTCCTGACCAGCACCGCACATTGTGCAAGAGATTCTACTACCTGTGGATCT
Seq A exon
ATATTACCTGCAGCTGATGCCTCCAAAG
Seq C2 exon
TGTACGGCGTGACCCTGCTGGTGAATTTCTTCGGCTGTATGGCGTGGATGTTTGGTGGCGGTGGAGTCACAAACTTTGGCATGTCGATGATTTGGGTGATTCTCTTCACACCCTGCTCTTATGTGTGCTGGTTCCGGCCCATTTACAATGCCTTCAA
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000040274_MULTIEX1-1/2=C1-2
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF041448=SCAMP=PD(95.1=95.1)
A:
PF041448=SCAMP=PD(8.7=66.7)
C2:
PF041448=SCAMP=FE(29.5=100)
Main Inclusion Isoform:
ENSDART00000058936fB5406
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Mouse
(mm9)
No conservation detected
Rat
(rn6)
No conservation detected
Cow
(bosTau6)
No conservation detected
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CCGTGTTTCCACCAGGACTTT
R:
CACGCCATACAGCCGAAGAAA
Band lengths:
125-153
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]