DreINT0035512 @ danRer10
Intron Retention
Gene
ENSDARG00000035565 | atp6v0a2b
Description
ATPase, H+ transporting, lysosomal V0 subunit a2b [Source:ZFIN;Acc:ZDB-GENE-060526-4]
Coordinates
chr5:24042528-24043899:+
Coord C1 exon
chr5:24042528-24042665
Coord A exon
chr5:24042666-24043801
Coord C2 exon
chr5:24043802-24043899
Length
1136 bp
Sequences
Splice sites
5' ss Seq
GAGGTGGGT
5' ss Score
7.07
3' ss Seq
TTTTGCTTGTGTGGGAGCAGAGA
3' ss Score
3
Exon sequences
Seq C1 exon
GAGCAGCTTCAGAGACTGGAACTCGAACTGGGTGAAGTCACCAGGAATAAGGAGAAGCTCCAGAAAAACCTTCTAGAGCTGACTGAATACACACACATGCTCCGCATCACTCGCAACTTTGTGCATCGCGGCACAGAG
Seq A exon
GTGGGTCATGTGATCCCTTCTCACATGTTGGCAGCTTTGGTTTGAATGGATCTTTTTTTTTTTTTTTTTACACAAAAGAAAAGGGGCATGTCTGACTGCTGAAAGGACTGGCTTAGTGCAGTCTGCTGTTTGGCTTGATCATGTTTATCCTCTAGTGTGAAAAGGAATTCATCCCTAGGCTGTAGGACTGTGGGAATCATGCAGGTGATTTAGAGTCACCTGATTGTCCAGAATTTTGAGAGCAGCCACCCGTGATCAAAAATATTAAGCTTAACACTTGACCTCTTCTTTTTGATCTTTTTGAAGCTGTGCGTGCGTGCATGCGTGCGTGCGTGCGTGTGTGTGTGCGTGTGTGCGTGCGTGTGTGTGTGTGTGTGTGTGTGTGTTGGGGGTTAAGTTATTACTCATATCGTGGGGACAAAAAGTGGTAAGACCTTAATTTTTGTGGAACTTGTTTTTATCTTGGTGAAAAGAGCAGAATGTTAAGCTTAATAAAATTGTTAATTTATGCATTCTTTCATTAATATCATAAATTAAATATTGTAATTAATAAATATATTTGGTGCATGCTGTGTATAGTGGTGTGCAGGGCTGGGCGATATGGCAAAAATGCAATCTCGATAACTATTTTCCATATTGAACAATAACCATATACATATTGATATCAGTTGTTCATGCTTCCAGATTTAACAGTACACCAACAATGACTAAAGCCATAAAAATTTGAGTCCATTAAATGGCCTAACATTTTCGGCTGATAAATTTTCCATGGCTGAAAGTTTGGTGCATCTTTAATATCAACACACTTTTAGATTCCCAATGTTGCACATTTCTGCTTTGTGATTGGCTCTGAGATCAATACAGAGTAAAAAAGTCATTGTCATTATGTGGTCTGTATTGTCTCTCCAATGTTACAGTTTTAAGCCGGTTGTAACATTGTTATTGACACAAATTTTAAACACGATTCGATATAATATCGTTTATCGGCCCAACCCTAGCGGTGTGGTTGGAATATACACCACTTGAACAGTGTAAAAATAAGTTTGTCTGTGAAAGGTCCCAATAAAACATAAAAACATGGACCTGCTTATGTGTATATCTTATCCCCTGCTTGGTTTTTGCTTGTGTGGGAGCAG
Seq C2 exon
AGAGAGTCAACACAGGGCCAGTATGAAGAATTCCCCTTTCTAGAGAAAGAACCCATGATGGACTACACCAGCATGCAGAGGCTTGGAGCCAAGCTTGG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000035565:ENSDART00000029699:4
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.030
Domain overlap (PFAM):
C1:
PF0149614=V_ATPase_I=FE(5.5=100),PF104734=CENP-F_leu_zip=FE(45.9=100)
A:
NA
C2:
PF0149614=V_ATPase_I=FE(3.9=100),PF104734=CENP-F_leu_zip=PD(9.2=27.3)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GAGCAGCTTCAGAGACTGGAAC
R:
AAGCCTCTGCATGCTGGTGTA
Band lengths:
222-1358
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]