DreINT0045468 @ danRer10
Intron Retention
Gene
ENSDARG00000036337 | cers3b
Description
ceramide synthase 3b [Source:ZFIN;Acc:ZDB-GENE-080220-32]
Coordinates
chr18:7361276-7362009:+
Coord C1 exon
chr18:7361276-7361368
Coord A exon
chr18:7361369-7361880
Coord C2 exon
chr18:7361881-7362009
Length
512 bp
Sequences
Splice sites
5' ss Seq
AAGGTAAGA
5' ss Score
10.57
3' ss Seq
GTGTGTGTGATTGTGTTTAGGAT
3' ss Score
9.11
Exon sequences
Seq C1 exon
TCATTGCTGGACTCTCAATACTGGTATTACATCACTGAGATGAGTTTTTATGGTTCTTTACTGTTCAGTGTGGCTGCAGACGTAAAACGGAAG
Seq A exon
GTAAGAAGACATCTATATATCAGTATTTCCATTGCCTCAACACATCATTTCATTATCGTTATCACTTAAATGATTATGCGTGAAGATATCAGTAGTCAAGTAGTAGGTCAAAGATCCTCAACTCTCTTTATCAACAAGCCTACAGGTAGCAGTTCTAATGTCATCAAACACCAAGAAAGTTGTTTTGCTTACATCTATCATAGTAATTATCACAATAGTTAACTAGAAGTATGAACTATTAATAACTGAGAATATGATGTCACAGTATCTTAAAAGATTAGTCTATCTAAGCTAGGCAAGTTTATATATATAGCACATTTCATACACAGTGGTAATTCAAAGTGCTTTACATAAACAAGAATAACAGAAACAAGAAAATAACAATGATAAAAACAGAATAAAATGTGTTAAAACAAGTTTTATAGGAATAAAAAAGAAAAGAAAGACATAAAAGTGCAATCTAGATACAGTCAGCTCTACTTCTCTCTCTCTGTGTGTGTGATTGTGTTTAG
Seq C2 exon
GATTTTAAGGAGCAGCTGGTGCATCACTGGGCCACTTTAACACTCCTGTCTTTCTCATGGTGTGCCAACTACATCCGTATTGGCACCCTGGTAATGCTGGTGCACGACACGTCTGACGTCCTGCTCGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000036337:ENSDART00000052792:7
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0379811=TRAM_LAG1_CLN8=FE(15.5=100)
A:
NA
C2:
PF0379811=TRAM_LAG1_CLN8=FE(21.6=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TCATTGCTGGACTCTCAATACTGG
R:
CTCGAGCAGGACGTCAGAC
Band lengths:
222-734
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]