DreINT0086856 @ danRer10
Intron Retention
Gene
ENSDARG00000040432 | klf2b
Description
Kruppel-like factor 2b [Source:ZFIN;Acc:ZDB-GENE-011109-2]
Coordinates
chr19:17832518-17835170:+
Coord C1 exon
chr19:17832518-17832839
Coord A exon
chr19:17832840-17834323
Coord C2 exon
chr19:17834324-17835170
Length
1484 bp
Sequences
Splice sites
5' ss Seq
GATGTAAGT
5' ss Score
9.11
3' ss Seq
TGTTTGTTTTGTTTTTTCAGAGA
3' ss Score
10.23
Exon sequences
Seq C1 exon
GCACTGAGAAACGAGAAGCGCTGGAGAGAGCGCACTGTGCCTTATATATAGACTATCTTTGGGAACTCTGAACGCGGTTTCCACCGCGCACACAATTGGTCTAGGAGCCGTATTTATTAAGTAGGAGGCGCTCCTGCCGGGCAAACTTTGTTCATGACTGTGGAAGTTCACTCGAGGAAAAAGCGCGCAGAAGTTTGACATCTCTTCAGGAGCAAGTGCATTAATACTGAATACACACCGAGCACGCGTGGACATGGCTTTACCTTGCCTTTTGCCTTCGATTTCAACGTTTGCAAACCAGAAGGAGAAGCTCTGGGAGGAT
Seq A exon
GTAAGTATTTGACACTTTTATGCACTTTTAAACGAGGGTGAAGTCAGGGCAATGCGGGAAACCTCACCTCAGATGTGGTTAAAATTGGGTTAACACTCGAAAAACACTTGCATTTCCTACTTGTTTTTGCGCAAAATACAATATATTGGAGATATAAGTCAAAAACACGCTTAAGAAATTTAGTTTTTATACGTATTTTATGCTTATAAGGTAAATATATCTTGTTTAAATGCATGTTTGATAAAAATGTTTAGTGCAAAACTTAATCGGAAAAGTGGGGGAAAGTAATGTTATAGTTTTTACTGTGTGTGTGTGTGTGTGTGTATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATGTGTGTGTGTGTGTGTGTGTGTATTTATAAAAACACACTAAATTATAATACACTATAGTAGGTTACTAAAACACTATACAAATTTGTATATGCAACTATACTATAATAATAACATAATAATTTAAAATAACTATTGATGTTGTTTTTAGTTCTTATTTAAACATTTATTTAGATAATGTAATTTTCATATACATATATGATATATACATAATGTCATCATTAAGTGAATCAAAAAGGTTTTCAAAACTGTCTTAAGACAAGAATGGGTGTTAAATAATTTAAGGACAACTATGATGAAAGTATTTTTTTTGATCCACTTTAAATAATTATTGCTGTAATCTGATTTTGTATCAGCATCAGAAAGAGCATTACTGCCAGGTGTGTTTACACATGTGAGGAATATAAATAAATATATACACTAGAAGTCAAAAGTTTGGGGTCAGTTTTTTTTTTATTCTTTATTTACTAATTTAAATAAAATTATTCTGTTCATCAAGGCGCCATTTATTAAATGTAAATGTTAAATTGTTAAATACGTATTTATTAAATATTTATTTATAACGTATTGTATGTAGATTTAATTGAAATTAATACTCCAGTCATTATTGCTTTTATTACTATTACCATTAATAATCAAATATTAATAATGATAATAAATAGTAGAGTGAAGGATCGTGTGACACTAAAGTAATGAAGCTGAAAATTCATCATTAAAATCGTTGCAATAAATGTTTAAATTCAATTATAAACTACTTTTGAGCAGTTATTTTATAGTGCAATAGCATTTCATAATCTCTACTGTATTTTTGCTGAAATAAATGCATTCTTAGTGAGCAAGAGAAGCTTATTTTAAAACATTTAAAAATCCTACTGACCCCAAACTTTTGACTGCTAGTGTATGCAAGGACATAAATCAAAATAACATCTGTAAAAAAAATCTGGTTTCATGATCTCTGTCTTAAAACAGTTTGATGTAAAGTTTCATATGTTGGTCTGAAGAGTTGAGGTGACAGCGAAACGCTCATGAAACGCTTTCAGGATGAGTCTTGCGAAAGTAGATTTCGTTCTAACCGCTCTCTGTTTGTTTTGTTTTTTCAG
Seq C2 exon
AGATGGAAGATTGAGGAGCACAAACCACTGAACGCCAGCTGCCCTCCGCTCAACGTGGCACTGAACACAGACACTTGTCGAGGCCGTAAGGATGACGAGGATCTCAGCAATTATTTAGACCTGGAGTTTATCCTGGCCAACACCACGGGCCCTCTGGAGGCCGGAGCCGAGTTCATCTCCCACTCCGAGCCATGCGGCATGTACTCCACTGCAGTTTACTCATCTCCAGAAGCACCTCCACCATACGGCCTCATGGCAGAGCTCCTGCGATCTGATGTAGACTCCACGTATGACACCACAGTGCAGGGGAGATTCCTGCTCAACTCCAGCGGCTTCCCCAGACAGGAGTTTCCTGAAATCAAAGTGGAGCCGCCGATGGATGGCTACGGTCCGGTGATAGGCATGGTGCCACAAACCTGCCAAAAAATCAAGCAGGAAGGCAACGTTTCATGCATGATGTCTTTCGAGCAGCCCAGACTAGCAGTTTCTCCGCAGGCCACCGGGAATATGACTCCTCCGCTGAGTCCTGACGACTCCCACCTCCGTCAAACGACATATACACAGAGCTACCATCACTCTCCTCCGGCGTATCCGCAGGTGCCAATGCAGTTCACCGCGCCACACCAGTTTGCCATGTATGAGGAGGCAATGGGGATGCAACCCAGCATGCAGCGGGCTCTTCTCACTCCTCCGTCCTCTCCGTTAGAGCTAATGGAGTCTAAACCCAAGCGAGGGCGACGCACCTGGCCGAGGAAGCGCATGGCGACACATACATGCACATACGCCGGCTGCGGGAAGACGTACACCAAAAGCTCCCATTTAAAAGCGCATCACCGCACTCACACCG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000040432:ENSDART00000149045:1
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion (1st CDS intron)
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.399
Domain overlap (PFAM):
C1:
NO
A:
NA
C2:
PF134651=zf-H2C2_2=PU(35.7=3.5)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Cow
(bosTau6)
No conservation detected
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TCTTTGGGAACTCTGAACGCG
R:
CTTACGGCCTCGACAAGTGTC
Band lengths:
357-1841
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]