DreINT0092541 @ danRer10
Intron Retention
Gene
ENSDARG00000099184 | map2k6
Description
mitogen-activated protein kinase kinase 6 [Source:ZFIN;Acc:ZDB-GENE-010202-3]
Coordinates
chr12:36456096-36458042:+
Coord C1 exon
chr12:36456096-36456173
Coord A exon
chr12:36456174-36457902
Coord C2 exon
chr12:36457903-36458042
Length
1729 bp
Sequences
Splice sites
5' ss Seq
ATGGTATGT
5' ss Score
8.35
3' ss Seq
GTGACGTGTGTGTTTATCAGATC
3' ss Score
8.43
Exon sequences
Seq C1 exon
CCTGAGAGAATCAACCCAGAGACCAATCAGAAAGGCTACAATGTCAAATCCGATATCTGGAGTTTAGGTATTACAATG
Seq A exon
GTATGTCTGAGCAACTTCATATACTTCTGATTTCTAATTAACTTCATTTATGAACACTTGTCAGGGTTTGTAAGGGTGCTGGAAATCTCTGAATTTTAATCTAGTGCTTTTAAGGTTTAAAAGTGTTTCTATTTTGGAGAAAGTGCTTGAAGATGTAATTGTGTTGGTTTAATGGTAATTTGTCTTAGTAAATGGGTACAAACAATTAACTAATTAAATAAAATAATACGCATCTTTTTGCTTTATCATTAAATGGAAACAAAATGCCTGCAGATTACCCTACTCATAAGTAAACAATGCATTGAATTAAAATTAATGTAAATGTAAGGTAACACATTTAAACTGGTTCAAAAATGAACTGGAAAGTGCTTAAATGTGGCTTTGGAAAAGCAGAAGAAACTCTGTAATGTTATCTTTATTGTTCAAAACTAGGTGGAGTTTTAGTTGTGGCTATACCATGCTAGTGCTAAAATAAAAATGCAAATATAAAATGTATTTAGATATATATTTATATTAAAAAATCATTTTTATTGTTTTGTTTTATTTTATTATATTATATTATTATTTTATTTTTAGAAAATATTTTTATCTTTTCTTTTTTTTCTTTTAATATTTTAATTTATTTACTTTTATAAATTTGTTTTTCTATTGTTATTGTTTTTATTTTATTTTATTTTTTATTATTTCGTTTAATATGTAATATTTTTGCTTTTCTTTTTTTCCTTTATTTAATTAAAATTATTTATTTATTTTTTATTTATTTTATATGATTTTTATTTTTAATAAAAATTTTGATTAATTTAATTTAGTTATTATTTTTTATTGTATTTTTTTATCTTATTGTTATTTTTTATTTTATTGATTTTTATTGATTTATTTTATTTTATACTTTATTATTATAATTTTTATAATTTTTTTTGCTATTTTATTTTATTGAAAAAACAGTGGTCAGAGTTGTAGAACTGCAATAACAGTTGCACAAAGAAAAATAACAGCTAAACTTTCTGAAGGTTTCAGCGACCCAAAATGTGTAAGGCATGTTCAGTAATCTCTAATTTCTCACAGGGCATTAGTAGTTTCCCCCATAGTCCTCCATCAGACCCAAGTAAATCAAACTTTCTTTCATCTTTAAAGTGATCTTTGGACCAGTTCTCTGTCCATACAACTTACATCTCAACAGATCAAGTTATCAAGGTCAAGTCTTAAAACCTTGCATCAGAAAATATATAAATCCTGAATCTGCTTAAAATCAGTGTGAAATCAAAATTTACAATGTTTGTTTTGCTAGCACACATTGTTATATTTTATTTGAACAATTAATTCATCTAAGATAATCCAATAATAAAAAAAGTTTTGTTTTGGTAATCTTTAATCAAAGTTTGACCATTTCTGTGTTTAGAGTTCTGGTCCTTTTTTGTTGACGTCATTTTGACTGCTTCCGCTTCAATATTCTTAACCACGCCCCTTTTACCGTTAGTTTGCCATAAGTGAATGATGCTCAAAAAGGAAGCCCCACCCACTACTCAACATTCCAATTTAGCTGGAAGTACATCAACATGTTTAGATAAAAGTCAACAATTTCCAGCTTGTGTGGCTCTTTTGCTTCTGTTTATAAATGTCACTTCTTGGTTTGACTTTTTAAAAGGGGGCAGAAATCTGTGACACCGACTGTGCTGCTCATAGCACGTTTGGTTTGTGTTGATAGTTATGTGACGTGTGTGTTTATCAG
Seq C2 exon
ATCGAGCTGGCGATTCTGCGGTTTCCCTATGACTCATGGGGGACGCCATTTCAGCAGCTAAAGCAGGTGGTGGAGGAGCCATCGCCTCAGCTGCCCGCCGACCGATTCTCTGCCGACTTCGTGGACTTCACATCACAGTG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000099184:ENSDART00000171268:9
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.269 A=NA C2=0.043
Domain overlap (PFAM):
C1:
PF0006920=Pkinase=FE(9.5=100)
A:
NA
C2:
PF0006920=Pkinase=FE(17.6=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGAGAGAATCAACCCAGAGACCA
R:
GTGAAGTCCACGAAGTCGGC
Band lengths:
207-1936
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]