DreINT0177864 @ danRer10
Intron Retention
Gene
ENSDARG00000077987 | zfat
Description
zinc finger and AT hook domain containing [Source:ZFIN;Acc:ZDB-GENE-130204-2]
Coordinates
chr16:25427120-25428509:+
Coord C1 exon
chr16:25427120-25427287
Coord A exon
chr16:25427288-25428347
Coord C2 exon
chr16:25428348-25428509
Length
1060 bp
Sequences
Splice sites
5' ss Seq
CAGGTAAGC
5' ss Score
9.88
3' ss Seq
AATTATTTTTACATTGACAGATG
3' ss Score
7.04
Exon sequences
Seq C1 exon
ATCCGCCATATTTGTTGTCGCAGATTCGACAGAAACCCCTGCAAGAAGTAATTTAAGCACAATGAATGCTTTAAAATGGCTCGATAGTTGTAAGAACAAAGATCAGAAATATTTCCGAGGTGTTTTTATGCATTACTGATTTAAAAAGAATGGATACAGCGCAAGCAG
Seq A exon
GTAAGCAGAAAGACGTTTTTTAGGCTAACTGCGCTCTGAGGAAACGGCTAACGTTAGTTCTGATGATTCCATAGGAGACAAACTAGGTAAAACTAGCGACTTGCACTTGCAAAAGTAACTTTTGCTAGACGATGCTTTATATTGAATTTAAAATTTAAGCTGTATGGTTGTAGTTGGTAGTTGAAATAACAGCGTTTCTAAACTAAACCTCACGTTGTTTATTAGAAACTAGTTCAAAGTGTCAAAGAGAAAAACACGAAACTGGTCTTGTGTTTAGAAAAATCGAAGGGCAGAGATTTGTTTCTCTTTTAGGAAAAAGAATGGGAGCATTAAAATATCAATAAACACTATGCAACATGTATTTGCTCTGGATAAAGTAATCTTTGATGTGACAGTTACACATGTCAAACGTATTGATTGATTACTTCTTGTGAAATAAATAAAATGTGTTGTGCTTATGATTAAATAACATTGTAATGACCATAATATTTGATTATTAACATCAATTTGAAATGGTTTAAAATCTAAACTCTTCCGAACTCCTTCAGGTCTTTAAGAAACAGTTGTTTAAGATCATAAAAAAACTATCAAAAAATAATAAAAATGTGTGGTTTATGGTAAATTCACAACAAAACACATAAAAATATTAATTCACATTATATTTTATTAGAGCCTGCTGTAATATTTACAACCTGCTTAAAGAGATTACAGAACAAAAAAATTAAAATGTACTTTTTACCATGCCTCAAGTGGTTCCAAACATTCATGATTTTCTTATGTTAAACAAAAAATAATCTATTTTGAAGAAAGCTGGAAACCTGTAACCTTTGACTTCCACAGAAGGAAAAATAAACACTATGGAAGTCGATGGTTACAGGTTTCCAGCATTCCTCAAAAGATCTTCTTTTGTGTTCAACATAAGAAAGAAACTCATGAATGTTTGAAACAATTAAAGGGTGTGTAAATGAGGACATAATGTTTATTTTTGTTTGAACTCGTTTCATAGTTATAACGTTACAGGATCATTTGATAAATATAATAATTATTTTTACATTGACAG
Seq C2 exon
ATGGTTCGTCAGTCTTCATGTGCAAAGGCTGCAACTTGTTCTCTCCCAAAAAAACACTGCTGCTCTCTCATGTTGTTGAGGCTCACTCCAGTGAAGGAGGAGATCCTGAGACCTTCATTATAGCACTTAAACCATTAACAACTCAGGAGACCCAGAATTCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000077987:ENSDART00000156351:1
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion (1st CDS intron)
No structure available
Features
Disorder rate (Iupred):
C1=0.429 A=NA C2=0.400
Domain overlap (PFAM):
C1:
NO
A:
NA
C2:
NO
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Human
(hg38)
No conservation detected
Human
(hg19)
No conservation detected
Mouse
(mm10)
No conservation detected
Mouse
(mm9)
No conservation detected
Rat
(rn6)
No conservation detected
Cow
(bosTau6)
No conservation detected
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TCCGCCATATTTGTTGTCGCA
R:
CTGAATTCTGGGTCTCCTGAGT
Band lengths:
329-1389
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]