GgaINT1021743 @ galGal4
Intron Retention
Gene
ENSGALG00000006237 | PKN2
Description
protein kinase N2 [Source:HGNC Symbol;Acc:HGNC:9406]
Coordinates
chr8:14124042-14124338:-
Coord C1 exon
chr8:14124202-14124338
Coord A exon
chr8:14124118-14124201
Coord C2 exon
chr8:14124042-14124117
Length
84 bp
Sequences
Splice sites
5' ss Seq
AAGGTAAAA
5' ss Score
8.38
3' ss Seq
GCGTGTGTTTATTTACATAGAAC
3' ss Score
6.06
Exon sequences
Seq C1 exon
GATGATGTATCAACTTTTGATTTTGAGAATGGTAGAAATAGTATTGTTCCAAAACTCCAGCCTGGAATAATCTGTGAGTCTGATGTTCCACATTCAGACATAAAATACACCAACATCCGAGAACCTGAAGATAGAAG
Seq A exon
GTAAAAATGCTATGGTTTTGGTGTTGAATATGCATAACCTTTATTATAGTCAGCTAACTGAAAAGCGTGTGTTTATTTACATAG
Seq C2 exon
AACACAACAAAGATTTCAATTCAGTCTGAAGGACTTCAGATGTTGTGCAGTACTTGGCAGAGGACATTTTGGAAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSGALG00000006237:ENSGALT00000010087:12
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.337 A=NA C2=0.038
Domain overlap (PFAM):
C1:
NO
A:
NA
C2:
PF0006920=Pkinase=PU(21.0=50.0)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGTTCCAAAACTCCAGCCTGG
R:
TTCCAAAATGTCCTCTGCCAAGT
Band lengths:
167-251
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]