GgaINT1024730 @ galGal4
Intron Retention
Gene
ENSGALG00000008297 | SEMA4B
Description
sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4B [Source:HGNC Symbol;Acc:HGNC:10730]
Coordinates
chr10:19532524-19533288:+
Coord C1 exon
chr10:19532524-19532681
Coord A exon
chr10:19532682-19533106
Coord C2 exon
chr10:19533107-19533288
Length
425 bp
Sequences
Splice sites
5' ss Seq
AAGGTGGGT
5' ss Score
8.23
3' ss Seq
GACCCCCAGTGCCCATACAGGGG
3' ss Score
2.36
Exon sequences
Seq C1 exon
ACCCGGTGTTCGTGGGCTCAGCCTTCCTGCAGGAGAGCCTGCCTGCCGGCAACCCCGATGGCGATGATGACAAGGTCTACTTTTTCTTCAGCGAGACCGGCAAGGAGTTTGACTACTTTGAGAACACCATTGTGTCACGCATCGCACGCGTGTGCAAG
Seq A exon
GTGGGTGCAAGGCAGTGGGGACGTGGTGGGGGGAATGCGCCCAGGCTGTCACTCATCCCCGGAGTGTGGGGAGGACATGGGACCGCCCTGTGTATCTCTGTCTGCTGGTTCCCAGACGCCCTGGGAAGGAGCCTGCAGGAGACCCCTCTATGGCACTTACAGCTCTGCAGTGCCCAGCCCATGGCATCGGTAGCAGCAGCCTCCTTGCTGGAGGGCACGCAGACCTATCCTCCTCCTGCACCGCATTTCCCTTCTCCTGGAAACACTGCTGTGCCTGTGCCTCCCCAAAACTGAGCTGCTCTGGTGCAGCCGCACGAGTGGGCAGAGCCCACTCACATTCACGGACATGGGGGGCATCTCGGAGCTCCTGCTGTGAGGCAGCCGCACTACCGTTGCCCATGCCCTGACCCCCAGTGCCCATACAG
Seq C2 exon
GGGGACCAGGGTGGGGAGCGGGTGCTGCAGCGGCGGTGGACGACGTTCCTGAAGGCGCAGCTGCTCTGCTCGCACCCCGAGGACGGCTTCCCCTTCAACGTGCTGCAGGACGTCTTTGTGCTCACGCCCGGGGAGCAGCGCTGGAGGGAGACGCTCTTCTATGGGGTCTTCACGTCGCAGTG
VastDB Features
Vast-tools module Information
Secondary ID
ENSGALG00000008297:ENSGALT00000013511:8
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0140314=Sema=FE(11.8=100)
A:
NA
C2:
PF0140314=Sema=FE(13.6=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GGTGTTCGTGGGCTCAGC
R:
CTGCGACGTGAAGACCCCATA
Band lengths:
334-759
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]