HsaINT0137391 @ hg19
Intron Retention
Gene
ENSG00000091428 | RAPGEF4
Description
Rap guanine nucleotide exchange factor (GEF) 4 [Source:HGNC Symbol;Acc:16626]
Coordinates
chr2:173853421-173855653:+
Coord C1 exon
chr2:173853421-173853567
Coord A exon
chr2:173853568-173855537
Coord C2 exon
chr2:173855538-173855653
Length
1970 bp
Sequences
Splice sites
5' ss Seq
AGGGTGAGT
5' ss Score
9.25
3' ss Seq
GGTGTAATTTGTATTTGCAGGAC
3' ss Score
7.32
Exon sequences
Seq C1 exon
GGTGTGGTCTGCACCCTGCATGAAGGAGATGACTTCGGCAAGTTAGCACTAGTGAATGATGCCCCACGAGCTGCCTCTATCGTCTTACGAGAAGATAACTGCCATTTCTTAAGAGTAGACAAGGAGGATTTCAACCGGATCCTAAGG
Seq A exon
GTGAGTCCAAAGCAAAGTGTGGCTGAGAGACAGCCCATTTTTTTCTTTAACTAAATAAGCAAAGCTTTGAGCATAGTCTTAATTGAGTAGTCCTGTGATGAATGAGTGCTCTAGGCACAAAGGGCCTTTGTTACAAACCGGTTATATTTAATGTGTTGGTATGCATTCTGGGATCCAGAGGTTGTATTCCAGTAAAGCTGCAAATGCAGGATGCTATGCTGGACCCAGTCCCTCTATGCATCCCTGCACTGGTCCATGAGAATTGACGATAAATAGGAATTGCCTTGGCAAAATGTCAGTCTGAGAAGTTTCTCTCTGACATCCTGGGCATCCTTCTTTTTGTCCTTCCCATCCCACTCTCCAACACAGTGGCAGCCCCCTCTGCCCCTTGTCTTTCTCTTTCCTAGACCTTCCTAGTTTCCACCTCCTTCTCCTCCCAAATCTGAGCCTGTCTCATATCATCATACATTCCCTTGATTTATTGAGTGATTACATTTTACTGAGAATTAACATCTATCATGGTTACTCCAACCAAGTATTTTCATTTTCAAGGGCTTTATCTCAAATTGTTACAAACTAAAGGAATTTCTGACATTGGCAGGCAGGTGAATAACTGGGGTGGTAGAGGGAACTATGCAATTGCACTTAAAAGTTGGCTCTGCAGATGAGCCTACTCTAAGCATATTAAGGAATTTGTGAGTGTGGGTGATGTTTAGAAAAAAATGGTCTCTGGTCTCTCCCCAGCATGCGGTCCCTTGTCCCTGGGAGGGATGACTCTGCTTTGGCTGCTTGAGGCCTGATCATGGACTTTTAGGGATGGTGACGATGTCCCACCACCCGTGGCTTCTCAGGTGGCCTGTGGTCCTTCCCGCCCTAGCTTGAGTTCACTCAGGAGGCTGTTCTTTCTAGGGTCACACTGCCCCTCTTTCTTTCACTGCTGACCCACAGAGCAGGGTCCCTTCTGTCCCTCTTGCAGCCTTAGCATCCCTATCCCCTCCTGAAGTCCTAAATGTAGCTTTTGTGCTTTGGGGTATATTCCCGTTACTTAATGATGATTTACAATGTCATGCTCCTGCCCTTGTGTCTGGACATCAATCCCTACATGGCCTTAAGCATTTGCCTTTGGTGGCAAAATTTCTGACAACTAAGTTTGTCTTAGGAATGCATGGTCTTAATGCAAAAAAAAAAAAGAAAAGAAAAAAAAAGGCTAAAATGTATACCCTTGAATGATATATTAAAAAAATACCAGATGGAATTTGAGTTGGTTCTTATCTGACCTCTTTTTAAATGAGGGCCAAATGTTGAGGAAAAGCATGTTAAAATTTTTAAACTAACTATTTACAATGATATTGAAATTTAAAGGTAAACTGTACTAACTCCTTCAAGTTTACACACAAGGAGTTTACATTCAGCCAAAACATTCTTTGAACGAATCAGATTAGGACCATTATCATTGATGTAATTTATAAAATCACTTAAAATAACCAAAAATAGTTTTTAACACATTTCAAACAGATTTTTCTTAAAAACAAAACAAAACAAAACAAAGCAGTCTTTTGATTAAAGAGAAAAGGTGCCCCACTCCTAAGGAAACTAAATACACTTAGGAGAAAAAGAACTTTGCTGAAGCTGCTTGCTATTTACCACATATTTTTATAACTAATAAATGCCTTTGCTCCCCACCTTCCTGCTCTACTTCCTTCTCCCAAAGGTTTCTCAGAGCATAACCACACTATGCTTTATTCTGCTGTTTTTCTGAAGCTAGGGTAAAAGCAGTCTTAAAAACACATGCTTCAAACCTCATCTGGTCCTCTCTCTGACCGTTCCAGCCCTTGACTTTGAAAATGACTTAATTTGTAGACGCAACAATTAGCATGACAAGCACCAAATGAGTTTCTGAAGCATTTGAAAATTTTTTCATTGTCTGAGTAAGCGGCAGCATCTGTATGTGGTGTAATTTGTATTTGCAG
Seq C2 exon
GACGTGGAGGCGAATACAGTCAGACTTAAAGAACATGACCAAGATGTCTTGGTGCTGGAGAAGGTCCCAGCAGGGAACAGAGCTTCTAATCAAGGAAACTCACAGCCTCAGCAAAA
VastDB Features
Vast-tools module Information
Secondary ID
ENSG00000091428-RAPGEF4:NM_007023:14
Average complexity
IR-S
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.333
Domain overlap (PFAM):
C1:
PF0002724=cNMP_binding=FE(55.2=100)
A:
NA
C2:
PF0002724=cNMP_binding=PD(2.3=5.1)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
Associated events
Other assemblies
Conservation
Chicken
(galGal4)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GTGTGGTCTGCACCCTGC
R:
TTTTGCTGAGGCTGTGAGTTTC
Band lengths:
262-2232
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Autistic and control brains
- Pre-implantation embryo development
Other AS DBs:
FasterDB (Includes CLIP-seq data)
AS-ALPS (AS-induced ALteration of Protein Structure, links to PINs)
APPRIS (Selection of principal isoform)
DEU primates (Only for human)