MmuEX0011367 @ mm10
Exon Skipping
Gene
ENSMUSG00000027577 | Chrna4
Description
cholinergic receptor, nicotinic, alpha polypeptide 4 [Source:MGI Symbol;Acc:MGI:87888]
Coordinates
chr2:181037419-181043546:-
Coord C1 exon
chr2:181043387-181043546
Coord A exon
chr2:181041400-181041549
Coord C2 exon
chr2:181037419-181037570
Length
150 bp
Sequences
Splice sites
3' ss Seq
CTGAGTACCTTTCTCCACAGCCA
3' ss Score
7.04
5' ss Seq
AGCCTTACT
5' ss Score
-22.48
Exon sequences
Seq C1 exon
ATTCATCACCGCAGTGCTCCCAGGAGCTGGAACCCTCCTCAGAGCGTTCATAGGGAATGAAGTGCTGAGTGCTGAGGTGCCTGTGTGGGGAGAAAGGCAGAGCACTGACTGCCCCAGCTTTGCAAATTGATGGCAAAGAGGAGCACCCACAGGAAGTCAG
Seq A exon
CCATATCTGAATGACAGCACCTAACCAGAGTTCCAGGGAAGCTGGAAGAAACGCCAAGGGACCTGGATCATGGTTGGCCACTTCTATATATTTAAGAGGATCTAACACTGTGTAGTCCAGGCTGACCTCAAACTCACTATGTAACCCAGC
Seq C2 exon
CTAGCAGCCACATAGAGACCCGGGCCCATGCGGAGGAGCGGCTCCTGAAGAGACTCTTCTCTGGCTACAACAAGTGGTCTCGGCCAGTAGCCAATATCTCAGATGTGGTCCTTGTCCGCTTTGGCTTGTCGATTGCTCAGCTCATTGATGTG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000027577_MULTIEX1-1/5=C1-3
Average complexity
C1*
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=1.000 A=1.000 C2=0.025
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
PF0293118=Neur_chan_LBD=PU(18.8=76.5)
Main Inclusion Isoform:
ENSMUST00000124400fB1008
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AACCCTCCTCAGAGCGTTCAT
R:
AAAGCGGACAAGGACCACATC
Band lengths:
252-402
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types