Special

MmuEX0013380 @ mm9

Exon Skipping

Gene
ENSMUSG00000028715 | Cyp4a14
Description
cytochrome P450, family 4, subfamily a, polypeptide 14 [Source:MGI Symbol;Acc:MGI:1096550]
Coordinates
chr4:115163671-115164840:-
Coord C1 exon
chr4:115164686-115164840
Coord A exon
chr4:115164166-115164272
Coord C2 exon
chr4:115163671-115163804
Length
107 bp
Sequences
Splice sites
3' ss Seq
CAAGTGCTGTCATGAGATAGATG
3' ss Score
-1.6
5' ss Seq
AGAGTGAGT
5' ss Score
7.36
Exon sequences
Seq C1 exon
AAATTCCAAGTTGTATACTAAGGCTGTCGAGGATCTAAACAACCTGACTTTCTTTCGCCTGCGGAATGCCTTTTATAAGTACAACATCATCTACAATATGTCCTCTGATGGACGTTTGTCCCACCATGCCTGCCAGATTGCTCACGAGCACACAG
Seq A exon
ATGGAGTGATCAAGATGAGGAAGTCTCAGCTGCAGAATGAGGAAGAGCTGCAGAAGGCCAGGAAGAAGAGACACTTGGATTTCTTGGACATCCTCTTGTTTGCCAGA
Seq C2 exon
GGACCATCTGGGCCAGATGCCCTACACCACCATGTGCATCAAGGAGGCCCTGAGGCTCTATCCACCAGTAATATCTGTGAGTCGAGAGCTCAGCTCACCTGTCACCTTCCCAGATGGACGCTCCATACCCAAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000028715_MULTIEX1-2/3=1-C2
Average complexity
C3
Mappability confidence:
100%=100=100%
Protein Impact





	

	
ORF disruption upon sequence exclusion





No structure available

		

			
Features


			

				
Disorder rate (Iupred):
  C1=0.000 A=0.194 C2=0.000
 
                        

				
Domain overlap (PFAM):

					





						

							
C1:
PF0006717=p450=FE(11.5=100),PF0075813=EPO_TPO=PD(29.1=47.2)

							
A:
PF0006717=p450=FE(7.7=100)

							
C2:
PF0006717=p450=FE(9.9=100)

						

					

				








    
Main Inclusion Isoform:
ENSMUSP00000030487





     
                   









    
Main Skipping Isoform:
NA





     
      









    
Other Inclusion Isoforms:
NA
          









    
Other Skipping Isoforms:
NA
  



Associated events






Other assemblies


mm10




Conservation


Human
(hg38)
No conservation detected
Human
(hg19)
chr1:47400125-47400231 
Rat
(rn6)
No conservation detected
Cow
(bosTau6)
chr3:99925492-99925598 
Chicken
(galGal4)
chr8:20777189-20777295 
HIGH PSI
GgaEX7006928
([1])
Chicken
(galGal3)
chr8:22596872-22596978 
Zebrafish
(danRer10)
chr16:4801114-4801220 
HIGH PSI
DreEX0025521
(cyp4t8)
Fruitfly
(dm6)
No conservation detected




Primers PCR

	

		

			
Suggestions for RT-PCR validation

			

				
F: 
GGCTGTCGAGGATCTAAACAACC

				
R: 
TCTGGGAAGGTGACAGGTGAG

				
Band lengths: 
248-355

			

		

	





	
Functional annotations
(Submit an annotation)

	


There are 0 annotated functions for this event 


		










GENOMIC CONTEXT[edit]












INCLUSION PATTERN[edit]






SPECIAL DATASETS
  • Pre-implantation embryo development
  • Neural differentiation time course
  • Muscular differentiation time course
  • Spermatogenesis cell types
  • Reprogramming of fibroblasts to iPSCs
  • Hematopoietic precursors and cell types






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