MmuEX0026155 @ mm10
Exon Skipping
Gene
ENSMUSG00000015714 | Cers2
Description
ceramide synthase 2 [Source:MGI Symbol;Acc:MGI:1924143]
Coordinates
chr3:95321575-95322233:+
Coord C1 exon
chr3:95321575-95321667
Coord A exon
chr3:95321831-95321959
Coord C2 exon
chr3:95322127-95322233
Length
129 bp
Sequences
Splice sites
3' ss Seq
AGCCTCTATCTTTTCTGCAGGAT
3' ss Score
9.17
5' ss Seq
GAGGTTAGG
5' ss Score
4.36
Exon sequences
Seq C1 exon
AGCATCATCCCTTCTCAGTATTGGTACTACATGATTGAACTTTCCTTCTACTGGTCCCTGCTCTTCAGCATTGCCTCTGATGTCAAGCGAAAG
Seq A exon
GATTTTAAGGAACAGATCATCCACCATGTGGCCACTATCATTCTCCTCTGCTTCTCCTGGTTTGCCAATTACGTCCGGGCAGGGACCCTCATCATGGCTCTGCATGACGCTTCTGACTACCTGCTGGAG
Seq C2 exon
TCTGCCAAGATGTTTAACTACGCGGGATGGAAGAACACCTGCAACAACCTCTTCATTGTGTTCGCCATCGTTTTCATCATCACTCGGCTGGTTATCATGCCTTTCTG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000015714-'17-23,'17-22,21-23
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF0379811=TRAM_LAG1_CLN8=FE(15.5=100)
A:
PF0379811=TRAM_LAG1_CLN8=FE(21.6=100)
C2:
PF0379811=TRAM_LAG1_CLN8=FE(18.0=100)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GCATCATCCCTTCTCAGTATTGGT
R:
AAGGCATGATAACCAGCCGAG
Band lengths:
195-324
Functional annotations
There are 1 annotated functions for this event
PMID: 33568634
Differential AS-based survival analysis shows that this AS event of CERS2 is a poor prognostic factor for Luminal B patients. As Exon 8 corresponds to catalytic Lag1p domain, overexpression of AS transcript of CERS2 in Luminal B cancer cells leads to a reduction in the level of very-long-chain ceramides compared to overexpression of protein-coding (PC) transcript of CERS2. The authors further demonstrate that this AS event-mediated decrease of very-long- chain ceramides leads to enhanced cancer cell proliferation and migration. Therefore, these results show subtype- specific AS of sphingolipid genes as a regulatory mechanism that deregulates sphingolipids like ceramides in breast tumors, and can be explored further as a suitable therapeutic target.
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types