MmuEX1018850 @ mm10
Exon Skipping
Gene
ENSMUSG00000034187 | Nsf
Description
N-ethylmaleimide sensitive fusion protein [Source:MGI Symbol;Acc:MGI:104560]
Coordinates
chr11:103857825-103859028:-
Coord C1 exon
chr11:103858895-103859028
Coord A exon
chr11:103858551-103858647
Coord C2 exon
chr11:103857825-103857925
Length
97 bp
Sequences
Splice sites
3' ss Seq
TTTTGGTGTTGTCTTTTAAGGCA
3' ss Score
8.37
5' ss Seq
CGAGTGAGT
5' ss Score
8.49
Exon sequences
Seq C1 exon
GGCCTCCTCACAGCGGGAAGACTGCCCTGGCTGCAAAGATTGCCGAGGAATCCAACTTCCCCTTCATCAAGATCTGCTCACCTGATAAGATGATTGGCTTCTCCGAGACAGCCAAGTGTCAGGCCATGAAGAAG
Seq A exon
GCAGGGTTTCTCTGTGAGCCCCTGGCTGTCCTGGAAAACTCTGTCAACCAGACTGGCCTCAGATTGACAGAGAGATCTGTCTACTTCGGCCTCCCGA
Seq C2 exon
GCAACCCCCCAAGCCACCAGCGTTGGAACCCTTGGAATCTGGAGTCACTGGACTTCATACCTTATGGAAGAATGGGCAATACTTACATTGTCCTAAACCAT
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000034187-'39-47,'39-46,40-47
Average complexity
C3
Mappability confidence:
100%=100=100%
Protein Impact
In the CDS, with uncertain impact
No structure available
Features
Disorder rate (Iupred):
C1=0.052 A=0.000 C2=NA
Domain overlap (PFAM):
C1:
PF0000424=AAA=FE(33.6=100)
A:
PF0000424=AAA=PD(3.8=6.1)
C2:
NA
Main Inclusion Isoform:
ENSMUST00000103075fB13812
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Human
(hg38)
No conservation detected
Human
(hg19)
No conservation detected
Cow
(bosTau6)
No conservation detected
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CTCCTCACAGCGGGAAGACT
R:
AGATTCCAAGGGTTCCAACGC
Band lengths:
171-268
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types