MmuINT0032424 @ mm9
Intron Retention
Gene
ENSMUSG00000026399 | Cd55
Description
CD55 antigen [Source:MGI Symbol;Acc:MGI:104850]
Coordinates
chr1:132344905-132346140:-
Coord C1 exon
chr1:132345994-132346140
Coord A exon
chr1:132344986-132345993
Coord C2 exon
chr1:132344905-132344985
Length
1008 bp
Sequences
Splice sites
5' ss Seq
CAGGTACAA
5' ss Score
7.09
3' ss Seq
CACTTCTGCTCATATTACAGCAA
3' ss Score
6.06
Exon sequences
Seq C1 exon
AGAAATCCAAGGTCCCAACGAAGAAACCAACAATTAATGTTCCAAGTACAGGAACCCCCTCAACGCCTCAGAAACCCACAACAGAAAGTGTTCCAAATCCAGGAGACCAACCAACTCCTCAGAAACCTTCCACAGTTAAAGTTTCAG
Seq A exon
GTACAAGAGTCCCAGCAATGCCTCAGAAACCCACCAGAGTTCATGTTCTAAGTACCAGAGTGCCACCAATGTCTCAGAAACCAAGCATGGTTAATGTTCCTGATACAAGAGTCTCACCAAAACCTCAGAAACCTACCAAAGCAAATATTCCTTCTACAGCAGCCCCACCAAAATCTCAGAAACCTACCAAAGCAAATAATCCATCTACAGCAGCCCCACCAACACCTCAGAAAACCAACACAGCAGATGTCCCAGCTGCCGAAATCCCACCAACACCTCAGAAAACCAACACAGCAGATGTCCCAGCTACAGAAACCCCAACATCTCAAAAAGCCAACACAGCACATGTTTCTGCTACAGAAATCCCATCAACACCTCAGAAAATGAACACAGCAGTTGTCTCAGCTACAGAAGTCTCACCTCAGAAAGCCAACACAGCACATGTTTCTGCTACAGAAATCCCACCAGCACCTCAGAAAACCAACATAGCAATTGTCCCAGCTACAGAAGCTTCACCAACACCAAAGAAAGCCAACACAGATGTCCCAGCTACAGAAATCCCACCAACACCTCAGAAAACCAACATAGCAATTGTCCCAGCTACAGAAGCTTCACCAACACCAAAGAAAGCCAACACAGATGTCCCAGCTACAGAAATCCCACAAACACCTCAGAAAATGAACACAGCAATTGTCCTAGCTACAGTAGAAGCCTCACCAACACCTCAGAAAACCAACACAGCAAAAAGTTCAGCCACAAAACCAGCAACAGCTCAGATGTCCCTCATATCAACTATTGAATCTGCACAAACCCCACCAATAACCCAGCATCCCACCACAGTTAATTCTTCTGTTGTACAAACTGCACAAACAGCCCAGACATTTGCCACAGCAAAACCTTCATTGACACAGAATTCACCAGCTACACAAAGGCCCACCATTTCACACTTCTCAATGACCAAGGGTCTCCATATTGTTACACAAAGATTCACTTCTGCTCATATTACAG
Seq C2 exon
CAACCCAGCATGTACCTGTTACCAAGACAACAGTACGTCATCCAATAAGAACATCTACAGACAAAGGAGAGCCTAACACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000026399-Cd55:NM_010016:7
Average complexity
IR-C
Mappability confidence:
NA
Protein Impact
No protein impact description available
No structure available
Features
Disorder rate (Iupred):
C1=0.990 A=NA C2=1.000
Domain overlap (PFAM):
C1:
NO
A:
NA
C2:
NO
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Rat
(rn6)
No conservation detected
Chicken
(galGal4)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TCCAAGGTCCCAACGAAGAAAC
R:
TGTGTTAGGCTCTCCTTTGTCTG
Band lengths:
222-1230
Functional annotations
There are 1 annotated functions for this event
PMID: 16503113
Transfection of isoform vDAF1, 2, and 3 cDNAs into CHO cells showed that these molecules are soluble forms secreted after glycosylation. Isoform vDAF4 and vDAF5 cDNAs included a part of and the entire intron 7 (HsaINT0031209) sequence, respectively, and the transfection of vDAF4 cDNA produced a large, glycosylated, membrane-bound form. vDAF4 has an Alt5 extending exon 7 nearly the entirety of intron 7 and/or it's an exitron of intron 7 retention.
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
Other AS DBs: