MmuINT0036207 @ mm9
Intron Retention
Gene
ENSMUSG00000027107 | Chrna1
Description
cholinergic receptor, nicotinic, alpha polypeptide 1 (muscle) [Source:MGI Symbol;Acc:MGI:87885]
Coordinates
chr2:73408464-73409665:-
Coord C1 exon
chr2:73409470-73409665
Coord A exon
chr2:73408702-73409469
Coord C2 exon
chr2:73408464-73408701
Length
768 bp
Sequences
Splice sites
5' ss Seq
CCGGTAGGT
5' ss Score
9.39
3' ss Seq
GACCTTGTTTTGATTCTCAGGAA
3' ss Score
8.29
Exon sequences
Seq C1 exon
CGCAGACGGCGACTTTGCCATTGTCAAATTCACCAAGGTGCTCCTGGACTACACCGGCCACATCACCTGGACACCGCCAGCCATCTTTAAAAGCTACTGTGAGATCATTGTCACTCACTTTCCCTTCGATGAGCAGAACTGCAGCATGAAGCTGGGCACCTGGACCTATGACGGCTCTGTGGTGGCCATTAACCCG
Seq A exon
GTAGGTGGTGGCCTCCTGGGATGCAGCTGGCTTCAGGACTAGGGCCACCTAGGTGTCCAAGTCAGCCTCTGGACAATCACAGGCTCCTGGCACCACTCTGAGCTAGAGAAGCATCTAGTGTATGGCTTTCAGCTGAGTGCCAAGGTTCTTTTTTTTTTTTTTTTAAATTATATATATATGTGTGTGTGTGTACATATATATACATACATATATCTCTGTGTGTATACATATATATATACATATACATATATATGTATACACACATACACACACTGTAGACGTTTACAGACACACTAGAAGAGGGAGGACATCAGCTCTCATTACAAATGGTTGTGAGCCACCATGTGGTTGCTGGGACTTGAACTCAGGATCCCTGGAAGAGCAATCAGTGCTCTTAACTGCTGAGCTATCTCTCCAGCCCCTGAGTGCCAAGGTTCTTAATACACTACTCAGGAGGGCTAAGTAAGAGTTTAAATAAATTCTCTTCGTTATGTGCTGTCTCCAAGAAGTCTGATAAAAACGAGTCTTGAGTGCAACTCAGGGGCCAGTGTTTTAAAGTTCCCTGGAGATTTGAGTGCAGAGCCAAAATGAACAACCCTAACTTACTATATGACGTGTTCTATAGGAAATAAACCTATTACCCCAAAGCAGAGGTAGCCCAACATCCCTCTTGGCAAAGTGACCCTCCGTATGGTACATGGCTACCATGTCTCTGCCCTTCCTCGGGGTGGTGTAGCAGGAGTCTTCAGACCTTGTTTTGATTCTCAG
Seq C2 exon
GAAAGTGACCAGCCCGACCTGAGTAACTTCATGGAGAGCGGGGAGTGGGTGATCAAGGAAGCTCGGGGCTGGAAGCACTGGGTGTTCTACTCCTGCTGCCCCACCACTCCCTACCTGGACATCACCTACCACTTCGTCATGCAGCGCCTGCCCCTCTACTTCATTGTCAACGTCATCATTCCCTGCCTGCTCTTCTCCTTCTTAACCAGCCTGGTGTTCTACCTGCCCACAGACTCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000027107-Chrna1:NM_007389:5
Average complexity
IR-S
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0293118=Neur_chan_LBD=FE(31.2=100)
A:
NA
C2:
PF0293118=Neur_chan_LBD=PD(24.0=62.5),PF0293211=Neur_chan_memb=PU(15.3=27.5)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CCAAGGTGCTCCTGGACTACA
R:
GAGCAGGCAGGGAATGATGAC
Band lengths:
356-1124
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types
Other AS DBs: