MmuINT0089460 @ mm9
Intron Retention
Gene
ENSMUSG00000024421 | Lama3
Description
laminin, alpha 3 [Source:MGI Symbol;Acc:MGI:99909]
Coordinates
chr18:12639395-12640656:+
Coord C1 exon
chr18:12639395-12639606
Coord A exon
chr18:12639607-12640451
Coord C2 exon
chr18:12640452-12640656
Length
845 bp
Sequences
Splice sites
5' ss Seq
AGCGTAAGT
5' ss Score
10.07
3' ss Seq
GTCTCTTTATGCCTTGGCAGCTT
3' ss Score
6.87
Exon sequences
Seq C1 exon
TCCCCAGGCAGCCTCTGGATTCTGTAAGAATTCTGCAAGGTCCCTGGTAGCCTTTTACCATAACGGTGCCATACCCTGTGAGTGCGACCCTGCTGGGACTGCCGGCCACCACTGTAGTCCTGAGGGTGGGCAGTGCCCTTGCCGGCCCAATGTCATCGGGAGGCAGTGCAGCCGCTGTGCGACAGGCTACTATGGATTCCCATACTGCAAGC
Seq A exon
GTAAGTGAGCACTGCTGAGTTTCTGAAGCCTCCTCCTGTCCCCAGGGCACGATGCTTGTGCACGGCACGACTTCTCAAACACCACTGATTTTTAGATGCACCCTTGGGGGCTTACGGTAAGGTTCTGATTCAAGAGATCTAGTCTGGGCCTAAAACTCTGCCCTTCTAATAAATGCCCAGGCTATCTCATGTATATGTCTATACTCTGAGCAGCCAGGCTCTAGGCTATGAATATAAATATAAACATAAACATAAAGGTGAGTGTATATGTGTTGCATGTGAGCAGCTCATTCCTAGCAGAAGCACTTCCAGGTTGTGTGGCCCCATGAAGCCTCAGTTTAGATAGGACGTATTACTCACTAACTTAGAAACCCTTGCATTTTTTACTTAAGAATCACCCTGTGACCTGATACCCTGGGTTTGCCCATGATGTAAATGGCAGTGAGTGACACAGGTGTGCAGACAGAGCACCGCAAAGGGACCAGTCTTTGAATAAACAAAAACAATCTCACTCCTGCACAATGTGCTGACCCTGGACCCGAGAGCTCTGGAAGGTGGGGGCTGCTTCAGAATGTCTTGAACAGTCACTACTTCATGCCTTCTAGAGCCTTCTGATGATCAGGATAGATCGGGAGCGGGGAGGTAGCAGCCTGGTTGGTTCATGGCGTGTGTAACCTGGAGGCAGTTACCCCAGCTGTCTCTCACTTGCTATGAGCAGTTCAGATCACCATGGGCAATGGCAAGGGAAAGAAAAACAATTCCCCAGACGTCTTGGGAACTTATGGAAGTCCCCATGGTTTGTGCAGTCCAACTCTCTCACCCAGTGTCTCTTTATGCCTTGGCAG
Seq C2 exon
CTTGTAATTGTGGCAGACGCCTTTGTGAAGAGGTGACAGGGAAGTGTCTCTGCCCACCCCACACAGTCAGGCCTCAGTGTGAGGTCTGTGAGATGAATTCCTTCAACTTTCACCCTGTGGCTGGCTGTGACGTCTGCAACTGCTCCAGGAAGGGCACCATTGAGGCGGCCGTCTCTGAGTGTGACAGGGACAGCGGGCAGTGCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000024421-Lama3:NM_010680:31
Average complexity
IR-S
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0005319=Laminin_EGF=PU(91.8=62.5)
A:
NA
C2:
PF0005319=Laminin_EGF=PD(6.1=4.3),PF0005319=Laminin_EGF=PU(46.9=33.3)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AGGCAGCCTCTGGATTCTGTA
R:
GAGCAGTTGCAGACGTCACAG
Band lengths:
352-1197
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types
Other AS DBs: