MmuINT0089619 @ mm9
Intron Retention
Gene
ENSMUSG00000015647 | Lama5
Description
laminin, alpha 5 [Source:MGI Symbol;Acc:MGI:105382]
Coordinates
chr2:179911979-179912349:-
Coord C1 exon
chr2:179912196-179912349
Coord A exon
chr2:179912113-179912195
Coord C2 exon
chr2:179911979-179912112
Length
83 bp
Sequences
Splice sites
5' ss Seq
TAGGTATGT
5' ss Score
7.76
3' ss Seq
CTCACCACTCCTCCCCACAGAGC
3' ss Score
10.19
Exon sequences
Seq C1 exon
GTGTCTGTGGGGTTCAGCGGCTGTCTGAAGAAATTACAGCTGGATAAGCGGCCACTGAGGACCCCAACGCAAATGGTGGGGGTCACACCCTGTGTCTCAGGCCCCCTGGAAGATGGCCTGTTCTTCCCAGGCAGTGAGGGAGTTGTCACATTAG
Seq A exon
GTATGTGAGGCAAGGGAGGTCTTGTGGGAATAGGAGTTCTAAGCCAGGCCCTGGAAGTCTCCCCTCACCACTCCTCCCCACAG
Seq C2 exon
AGCTCCCCAAGGCCAAGATGCCCTATGTGAGCCTGGAGCTAGAGATGCGGCCCTTGGCAGCTGCTGGCCTCATCTTCCACCTGGGCCAGGCCCTGGCCACTCCCTACATGCAGCTGAAGGTGCTGACAGAACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000015647-Lama5:NM_001081171:75
Average complexity
IR-S
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.038 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0221019=Laminin_G_2=PD(12.2=30.8)
A:
NA
C2:
PF0221019=Laminin_G_2=PU(23.4=64.4)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GTTCAGCGGCTGTCTGAAGAA
R:
GGCTCACATAGGGCATCTTGG
Band lengths:
176-259
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types
Other AS DBs: